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Tests and Fees

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Submission Fee

A $10 submission fee is assessed on each case.

Testing Schedule

Our goal is to provide accurate results and timely service. However, many diagnostic assays are not performed daily and/or testing schedules may change. If your case involves testing animals for export, testing show animals, testing animals to be sold, and/or testing a large number of samples, please contact the VDL (515-294-1950) prior to submission to verify the availability and turnaround time of specific assays. A phone call will often shorten turnaround time by allowing us time to prepare for testing prior to receipt of samples.

General Fees

Fees are subject to change without notice.

Please call the lab (515-294-1950) with any questions or for clarifications.

For further submission details see the ISU VDL User Guide.

  Download a printable version of the Fee Schedule.pdf.

  FEES      updated 10/6/2011
Submission fee each case $10.00   
ISU Environmental Surcharge 1% (not including submission fee)
Report by FAX per transmission $1.00  
Handling charge for leaky package $25.00  
Bacteria isolate     
     Slant preparation $30.00  
     Slant saved at ISU VDL $4.00  
     Slant shipped $10 - 25.00  
Shipping    
     Bacterial slant $10 - 25.00  
     Virus $75.00 plus $30.00 if virus needs to be propagated 
     Forwarding samples to outside lab $10- 20.00  

 

Bacteriology

  FEES         
Routine isolation - aerobic & anaerobic        
     1st sample $13.00      
     Additional specimens (each) $2.00      
Bacterial identification $3.00-10.00      
Susceptibility testing     
     Sensititre - MIC $18.00      
     Kirby Bauer $12.00      
     E test (for anaerobes) $18.00      
Brachyspira culture* $10.00      
Campylobacter culture* $7.00      
Clostridium    
     C. difficile culture* $12.00      
     C. difficile - toxin A/B ELISA $26.00      
     FA (including C. novyi, chauvoei,
     sordelli, septicum
)
$10.00      
Coliform culture* $15.00      
Direct microscopic exam - Gram's, KOH,
     Acid fast, Darkfield, etc.
$6.00      
Enrichment culture - Erysipelas, Salmonella,
     Listeria, etc *
$20.00      
Fungal culture        
     1st specimen $17.00      
     Additional specimens (each) $10.00      

Johne's - Mycobacterium paratuberculosis
     Liquid culture

$25.00
single sample
$27.00 pooled
     
Milk culture* $8.00      
     Bulk tank quantitation $16.00      
Mycoplasma culture        
     Culture - bovine $15.00      
     Culture - porcine $30.00      
     Identification- bovine $28.00      
     Identification - porcine $25.00-35.00      
Poultry Salmonella - FDA    
     Group D bacterial screen $30.00      
     Culture confirmation $30.00      
     Identification (NVSL) $30.00      
Poultry Salmonella - USDA
     Bacterial culture $11.00-33.00      
Serotyping        
    APP, Streptococcus suis $12.00      
    E. coli K99 $10.00      
    Salmonella and Strep spp. $10.00      
Slant request of isolate $30.00        
Shipping Fee $10.00-25.00      
Special culture - nasal, feces, semen* $6.00      
Standard plate count culture $20.00      
Trichomonas culture* $7.00      
Ureaplasma culture* $10.00      
     * per specimen

 

Chemistry - see fees

 

Molecular Diagnostics

  FEE     DAYS TESTED  TURNAROUND SAMPLE
PCR        
     AIV $35.00 as needed  2 days fecal, cloacal or oropharyngeal swab

     Avian paramyxovirus-1 matrix gene -  
     Newcastle disease

$30.00 as needed    
     APP $25.00 M, Th    
     Actinobacillus suis $25.00 T, F same day lung
     BCV - included in the bovine enteric panel $30.00 T, F same day feces, intestine

     Bovine enteric panel - BEP
          (Rotavirus group A,
          BCV, Salmonella spp., E coli K99,
          Cryptosporidium parvum)

$50.00 daily same day

feces, intestinal content

SEE DISEASE INFO

     Brachyspira $30.00 M, Th 1-2 days culture, feces
     BRSV $30.00 T, F same day lung, nasal swab
     BVD $30.00 T, F same day serum, fresh ear notches, blood, tissues, feces, milk, semen
     BVD genotyping $25.00 as needed   isolate
     Canine coronavirus $30.00 as needed   feces, intestine
     Canine distemper virus $30.00 M, Th   lung, blood, conjunctival swab, brain
     Canine herpesvirus $30.00 M, Th    
     Canine influenza $40.00 as needed   swabs, tracheal wash, fresh tissues
     Caprine herpesvirus $30.00 M, th    
     Clostridium perfringens -
          toxin gene typing
$30.00 M, Th same day culture
     EAdV1 - Equine adenovirus 1 $30.00 T, F same day nasal swab, conjunctival swab, tracheal aspirates, lung
     EAV - Equine arteritis virus $30.00 T, F same day nasal swab, EDTA blood, lung, semen, fetal tissues (lung, liver, kidney, thymus, spleen, placenta)
     E. coli genotyping $30.00 M, Th same day culture
     EEE, WEE (NVSL) $54.00 contact NVSL brain
     EHV1 neuropathotyping $35.00 T, F same day nasal swab, EDTA blood, lung, brain, cerebral spinal fluid, fetal tissues (lung, liver, kidney, thymus, spleen, placenta)
     EHV1&4 - Equine herpesvirus type 1&4 $35.00 T, F same day nasal swab, EDTA blood, lung, brain, cerebral spinal fluid, fetal tissues (lung, liver, kidney, thymus, spleen, placenta)
     EHV2 - Equine herpesvirus type 2 $30.00 T, F same day nasal swab, conjunctival swab, tracheal aspirates, lung
     EHV3 - Equine herpesvirus type 3 $30.00 T, F same day swab/scraping from reproductive tract
     EIV - Equine influenza virus $30.00 T, F same day nasal swab, tracheal washings, lung
     ERAV & ERBV - Equine rhinitis A and B $35.00 T, F same day nasal swab, conjunctival swab, tracheal aspirates, urine
     Erysipelothrix rhusiopathiae - 
          
tonsillarum & species
$30.00

M, Th

next day joint fluid, skin, spleen, lung
     Haemophilus parasuis $30.00 T, F same day lung, BAL, bronchial swab, joint fluid, pericardium (fluid/swabs), heart
     IBR virus - bovine herpesvirus 1 $30.00 T, F same day  
     Influenza A - screen and subtype $40.00 daily same day

lung, bronchial swab, BAL, oropharyngeal swab, nasal swab, nasal washing

SEE MORE INFO

     Influenza A - screen $30.00 daily same day lung, bronchial swab, BAL, oropharyngeal swab, nasal swab, nasal washing
     Influenza - pH1N1 differential $25.00 daily same day lung, bronchial swab, BAL, oropharyngeal swab, nasal swab, nasal washing
     Lawsonia intracellularis (Ileitis) $35.00 M, Th same day feces, fecal swab, intestinal contents
     Leptospirosis $30.00 T, F same day placenta, kidney, urine, semen, serum
     Malignant catarrhal fever virus - MCF $30.00 M, Th    

     Mycobacterium paratuberculosis
          (
Johne's)

$30.00
single sample
$35.00 pooled
M, Th same day feces
SEE JOHNE'S DISEASE INFO
     Mycoplasma bovis $25.00 T, F same day lung, BAL, milk, joint fluid
     Mycoplasma hyopneumoniae $25.00 daily same day lung, bronchial swab, BAL
     Mycoplasma hyorhinis $25.00 M, Th next day

joint swab or joint tissue

SEE MORE INFO

     Mycoplasma hyosynoviae $25.00 M, Th next day

joint swab or joint tissue

SEE MORE INFO

     Mycoplasma suis -
            (formerly Eperythrozoon suis)

$30.00 as needed   whole blood (EDTA or heparin), liver or spleen
     Pasteurella multocida - toxin gene $25.00 M, Th same day culture
     PCV type 1

$25.00 fluid
$30.00 tissue

as needed  

serum, BAL
lung, lymph nodes

     PCV type 2 - fluid $25.00 daily same day

serum

SEE DISEASE INFO

     PCV type 2 - tissue $30.00 daily same day lung, lymph nodes, tonsil, filter membrane (e.g. TEGO, FTA)
     PCV type 2 - oral fluid $30.00 daily next day

oral fluid

SEE MORE INFO

     PCV2a/b 
         multiplex differential

$25.00 serum, fluid
$30.00 tissue

as needed   serum, tissue, fluid
     PCV 1-2 $25.00 fluid
$30.00 tissue
as needed    
     Porcine cytomegalovirus - PCMV $35.00 T, F next day blood, nasal swab, turbinates, brain
     Porcine enterovirus - PEV
    
$25.00 T, F next day spinal cord, brain
     PPV $30.00 T, F next day fetal tissue
     PRRSV $25.00 daily same day

serum, tissue,
filter membrane (e.g. TEGO, FTA),

oral fluid
SEE MORE INFO

     PRV $30.00 as needed   brain, tonsil, lung, fetal liver
     Rotavirus - swine groups A, B, C $30.00 M, Th next day feces, intestinal contents, fecal swabs
SEE MORE INFO
     SHEV - Swine Hepatitis E virus $35.00 as needed   liver, serum, feces
     SIV - general and subtyping $40.00 daily same day

lung, nasal swab, BAL, oral fluids

SEE MORE INFO

     TGE/PRCV $30.00 daily same day feces, fecal swab, intestinal contents
     Tritrichomonas foetus $25.00 daily same day

bovine & feline feces only

inoculated trich pouch, preputial wash

SEE MORE INFO

     WNV $30.00 M, Th   serum, brain, spinal cord
     WNV (NVSL) $54.00 contact NVSL brain
PRRS RFLP Referred to outside lab
Quantitative PCR - PCV, PRRS       serum
     First sample $75.00      
     Additional samples (each) $25.00      
Sequencing and analysis    
     Influenza HA $110.00    

SEE DISEASE INFO

     PCV2 ORF2 $150.00 as needed 5-7 days

PCR-positive sample, isolate

SEE DISEASE INFO

     PRRSV ORF5 $110.00      

 

  FEE DAYS TESTED TURN-AROUND SPECIMENS
Electron microscopy (NVSL)
Antigen-capture ELISA
     Canine parvovirus - fecal testing $15.00   M, W, F same day feces
     Influenza A & B virus - swine, equine,
           avian, canine, feline
$18.00   M, W, F same day

nasal swab, tracheal wash, oropharyngeal swab

SEE DISEASE INFO

     Rotavirus - group A - bovine, swine $7.00   M, W, F same day feces
Rabies
     FA $30.00   daily same day  brain

     Emergency - weekend/after hours
          FA

$130.00   as needed same day  brain
Return of virus isolate
     Virus propagation when needed $30.00       
     Packaging and shipping $75.00       
Tissue section Fluorescent Antibody        
     BAdV - Bovine adenovirus $13.00  as needed same day lung
     BCoV - Bovine coronavirus $13.00 as needed same day lung, intestine
     BRSV - Bovine respiratory syncytial
                virus
$13.00  as needed same day lung
     BVDV - Bovine viral diarrhea virus $13.00 as needed same day tissues including tonsil
     CAdV - Canine adenovirus $13.00  as needed same day tonsil, lung, liver, intestine
     CCoV - Canine coronavirus $13.00  as needed same day intestine
     CDV - Canine distemper virus $13.00  as needed same day lung, brain
     CHV - Canine herpesvirus $13.00  as needed same day lung, spleen
     CPV - Canine parvovirus $13.00  as needed same day intestine
     EHV1&4 - Equine herpesvirus type 1&4 $13.00  as needed same day fetal tissues (lung, liver, thymus, spleen, placenta), lung
     EHV3 - Equine herpesvirus type 3 $13.00  as needed same day lung, spleen, lymph nodes
     EMCV - Encephalomyocarditis virus $13.00 as needed same day brain, heart
     FCV - Feline calicivirus $13.00  as needed same day lung
     FCoV - Feline coronavirus $13.00 as needed same day tissues including lung, liver, spleen, lymph nodes, intestine
     FHV - Feline herpesvirus $13.00  as needed same day lung
     FPV - Feline parvovirus $13.00  as needed same day tissues including intestine
     HEV - Hemagglutinating 
               encephalomyelitis virus
$13.00  as needed same day brain, spinal cord
     IBRV - Infectious bovne rhinotracheitis
                virus
$13.00  as needed same day lung, lymph nodes, spleen

     BPIV3 - Bovine parainfluenza virus
               type 3

$13.00  as needed same day lung
     PEV & PTV - Porcine enterovirus
               & porcine teschovirus
$13.00  as needed same day spinal cord, brain
     PPV - Porcine parvovirus $13.00 as needed same day fetal tissues, placenta
     PRRSV - Porcine reproductive and
               respiratory syndrome virus
$13.00  as needed same day lung
     PRV - Pseudorabies virus $13.00  as needed same day brain, tonsil, lung, fetal liver
     SIV - Swine influenza virus $13.00  as needed same day lung
     TGEV/PRCoV - Transmissible
              gastroenteritis virus/Porcine
              respiratory coronavirus
$13.00  as needed same day intestine (TGEV),
lung (PRCoV)
Virus isolation and identification  
     AIV - Avian influenza virus $50.00  as needed 7-21 days cloacal or oropharyngeal swab, feces, oral+cloacal, tracheal swab, environmental sample
     BPIV3 - Bovine parainflluenza virus
                  type 3
$25.00 as needed 7-14 days lung
     BVDV - Bovine viral diarrhea virus $25.00  as needed 7-10 days blood, tissues, serum, semen
     CAdV - Canine adenovirus $25.00 as needed 7-14 days tonsil, liver, kidney, spleen, lung
     CHV - Canine herpesvirus $25.00  as needed 7-14 days lung, spleen, blood. corneal swab
     CIV - Canine influenza virus $25.00  as needed 7-14 days

swabs, tracheal wash, fresh tissues (lung)

SEE MORE INFO

     CPV - Canine parvovirus $25.00  as needed 7-14 days intestine
     EHV1&4 - Equine herpesvirus 
                     type 1&4
$25.00  as needed 7-14 days fetal tissues (lung, liver, thymus, spleen, placenta), lung, blood, nasal swab
     EHV3 - Equine herpesvirus type 3 $25.00  as needed 7-14 days lung, spleen, blood, lymph nodes
     EIV - Equine influenza virus $25.00 as needed 7-14 days swabs, tracheal wash, fresh tissues
     EMCV - Encephalomyocarditis virus $25.00 as needed 7-14 days brain, heart
     FCV - Feline calicivirus $25.00  as needed 7-14 days lung, nasal or tracheal swab
     FHV - Feline herpesvirus $25.00 as needed 7-14 days lung, nasal or tracheal swab
     FPV - Feline parvovirus $25.00  as needed 7-14 days blood, tissues
     IBRV - Infectious bovine rhinotracheitis
                virus
$25.00  as needed 7-14 days lung, lymph nodes, blood
     PCV2 - Porcine circovirus type 2 $25.00  as needed 7-14 days

serum, lung, lymph nodes, tonsil

SEE DISEASE INFO

     PEV & PTV - Porcine enterovirus
               & Porcine teschovirus
$25.00  as needed 7-14 days spinal cord, brain
     PRRSV - Porcine reproductive and
               respiratory syndrome virus
$25.00  as needed 7-14 days serum, tissues, BAL, oral fluid, blood
     PRV - Pseudorabies virus $25.00  as needed 7-10 days brain, tonsil, lung, fetal liver
     SIV - Swine influenza virus $25.00  as needed 7-21 days lung, nasal swab, BAL, oral fluids
Virus quantitation - cell culture based $120.00   
 

Pathology

  FEE DAYS TESTED TURNAROUND
Biopsy $20.00    
BVD screen for PI by IHC                            SEE MORE INFO
      1-3 notches $11.00 M-F

next day

Use the Serum Submission Form 

      > 3 notches (each) $3.00 M-F next day
Disposal - equine diagnostic cases only* $60.00    
      *The VDL does not accept submissions for disposal purposes only.
Histopathology (each slide) $5.00    
Immunohistochemistry $13.00    
Necropsy  
     Fetus / embryo - immature $5.00    
     Juvenile / adult $10.00    
     Special or complex procedures $60/hr    
Parasitology      
      Baermann 1-5 samples    $15.00 M-Th next business day
      Baermann 6-10 samples $7.50   next business day
      Baermann >10 samples ** $5.00 M-Th  
      Direct Smear 1-5 samples $13.00 daily next business day
      Direct Smear >5 samples $6.00 daily next business day
      Fecal flotation 1-5 samples $15.00  daily next business day
      Fecal flotation >5 samples $8.00 daily next business day
      Quantitative McMaster float 
             1-5 samples
$15.00  daily next business day
      Quantitative McMaster float 
             6-10 samples
$7.50  daily next business day
      Quantitative McMaster float 
             >10 samples**
$5.00  daily  
      Sedimentation 1-5 samples $15.00 M-Th next business day
      Sedimentation >5 samples $8.00 M-Th next business day
** If submitting greater than 10 fecal samples, please contact the laboratory for scheduling, price quote, and approximately turnaround time.
Special Stains $13.00     

Serology

  FEE DAYS TESTED TURN-AROUND SPECIMEN
AIV - Avian influenza
     AGID - serum $2.50 M-Th 24-hour test serum
     AGID - egg yolk $2.50 M-W 48-hour test egg yolk
     ELISA $1.50  as needed same day serum
Ana - Anaplasma marginale
     CARD $4.00 as needed same day serum
     CF $4.00 T, Th same day serum
     ELISA $6.00  W same day serum
APP - Actinobacillus pleuropneumoniae
     CF 1, 5, 7 (no serotyping) $5.00 M, W 2-day test serum
     Mix APP ELISA $12.00 M same day

serum

SEE MORE INFO

     ELISA 10-12 $4.00     serum
     Serotype 1, 5, 7 $15.00 as needed   serum
     Serotype 3 $5.00  as needed   serum
APV - Avian pneumovirus
     ELISA $1.75 as needed same day serum
Baculovirus        
     ELISA $6.50 M, W, F same day serum
BLV - Bovine leukemia virus
     AGID $5.00 M-F 24-hour test serum
     ELISA $5.00     serum
BRSV - Bovine respiratory syncytial virus
     VN $5.00 W, F 7-day test serum
Brucellosis
     B. abortus PCFIA (NVSL) $38.00 contact NVSL   serum
     B. abortus/suis BAPA $1.00 M-F same day serum
     B. abortus/suis card test $2.00 M-F same day serum
     B. abortus FPA $7.50     serum    SEE MORE INFO
     B. abortus/suis RAP $1.00 M-F same day serum
     B. abortus/suis RIV $1.00 M-F same day serum
     B. canis RSAT, 2ME $10.00 M-F same day serum
     B. ovis CF (NVSL) $18.00 contact NVSL   serum
     B. ovis ELISA (NVSL) $18.00 contact NVSL   serum
     Bruc. SPT $2.50 M-F same day serum
     Bruc. STT $2.50 M-W 3-day test serum
BT - Bluetongue
     AGID $5.00 M-Th 24-hour test serum
     CF (NVSL) $18.00 contact NVSL   serum
     ELISA $8.00 F same day serum
     VN (NVSL) $14.00 contact NVSL   serum
BVD - Bovine viral diarrhea
     AgELISA - serum PI testing $6.00 M, W, F same day serum, fresh ear notch
     VN - type I & II $5.00/
each type
W, F 5-day test serum
CAE - Caprine arthritis encephalitis virus
     AGID $5.00 M-F   SEE MORE INFO
     cELISA $6.50 as needed same day serum
Canine influenza - H3N8    
     HI $10.00  as needed 24-hour test

serum

SEE MORE INFO

Chlamydia psittaci
     CF (NVSL) $18.00 contact NVSL   serum
CHV - Canine herpesvirus
     VN $5.00 T, F 3-day test serum
EAV - Equine arteritis virus
     VN (NVSL) $14.00 contact NVSL   serum
EEE/VEE/WEE
     CF (NVSL)

$18.00
each strain

contact NVSL   serum
     HI (NVSL) $15.00
each strain
contact NVSL   serum
     VN (NVSL) $14.00
each strain
contact NVSL   serum
EHD - Epizootic hemorrhagic disease
     AGID $5.00 M-Th 24-hour test serum
     CF (NVSL) $18.00 contact NVSL   serum
     VN (NVSL) $14.00 contact NVSL   serum
EHV - Equine herpesvirus (Eq. rhino.)
     VN Type 1, 2, 3, 4 (NVSL) $14.00 contact NVSL   serum
EIA - Equine infectious anemia
     AGID $6.00 M-F 24-hour test serum
     ELISA $6.00 M-F same day serum
EMC - Encephalomyocarditis virus
     VN (NVSL) $14.00 contact NVSL   serum
HEV - Hemagglutinating encephalomyelitis
     HI (NVSL) $15.00 contact NVSL   serum
HS - Haemophilus somnus
     CF $5.00 T, Th same day serum
IBR - Infectious bovine rhinotracheitis
     VN $5.00 T, F 3-day test serum
Johne's - Mycobacterium paratuberculosis - SEE JOHNE'S DISEASE INFO
     AGID $5.00 M-Th 24-hour test serum
     CF $5.00 F same day serum
     ELISA $5.00 W, F same day serum, plasma, milk
Lawsonia intracellularis
     ELISA - porcine only $8.00 as needed same day serum
Lepto - Leptospira interrogans
     MAT 5 serovars $5.50 T, Th same day serum
  5 serovars = canicola, ictero, grippo, pomona, hardjo
     MAT 6 serovars $6.00 T, Th same day serum
  6 serovars = canicola, ictero, grippo, pomona, hardjo, bratislava
MCF - Malignant catarrhal fever
     IPT (NVSL) $16.00 contact NVSL   serum
     VN (NVSL) $14.00 contact NVSL   serum
M hyo - Mycoplasma hyopneumoniae
     Dako ELISA $9.50 W same day serum
     ELISA $5.50 M-F same day serum
     Tween 20 ELISA $4.75 as needed same day serum
Neospora
     ELISA $6.00 F same day serum
OPP - Ovine progressive pneumonia AGID
     AGID $5.00 M-F same day serum
PCV2 - Porcine circovirus type 2   SEE MORE INFO: PCV2
     Antibody titration $15.00     serum
     ELISA $5.25 M, W, F same day serum
     FFN $16.00 T 3-day test serum
     Four dilution IFA $7.50     serum
PHF - Potomac horse fever
     IFA (NVSL) $15.00 contact NVSL   serum
PI3 - Parainfluenza 3 Virus
     VN $5.00 T, F 3-day test serum
PPV - Porcine parvovirus HI
     HI $5.25 M 24-hour test serum
PRRS - Porcine reproductive & respiratory syndrome
     ELISA X3 $5.00 M-F same day serum    SEE MORE INFO
     ELISA X3 - oral fluids $7.50 as needed next day

oral fluids
SEE MORE INFO

     FFN $15.00 T 3-day test serum
     IFA European - screen @ 1:20 $5.00 M-F same day  serum
     IFA European - titration $15.00 M-F same day serum
     IFA North American - screen @ 1:20

$5.00

M-F same day serum
     IFA North American - titration $15.00 M-F same day serum
     Homologous IFA - screen @ 1:20 $5.00 as needed 2-4 weeks virus culture & serum
     Homologous IFA - titration $15.00 as needed 2-4 weeks virus culture & serum
PRV - Pseudorabies
     ALA $3.00 as needed same day serum
     g1 ELISA $2.75 M-F same day serum
     gB ELISA (screen) $3.50 as needed same day serum
     VN $5.00 T, F 3-day test serum
Pullorum
     AGG $3.00 as needed same day serum
Q Fever (Coxiella burnetii)
     CF (NVSL) $18.00 contact NVSL   serum
SIV - Swine influenza virus - SEE DISEASE INFO
     H1N1 ELISA $4.75 T, Th same day serum
     H1N1 Pfizer HI $5.00 as needed 24-hour test serum
     H1N1(99) HI $5.00 as needed 24-hour test serum
     H3N2 ELISA $4.75 T, Th same day serum
     H3N2 HI $5.00 as needed 24-hour test serum
     NP ELISA $5.00 as needed same day serum
TGE - Transmissible gastroenteritis virus VN
     VN $5.00 W, F 5-day test serum
TGE/PRCV
     ELISA $9.00 T, Th same day serum
Toxo - Toxoplasma gondii
     LAT $5.00 M-Th next day serum
VS - Vesicular stomatitis
     cELISA (NVSL) IN & NJ $36.00 contact NVSL   serum
     CF (NVSL) - IN & NJ $36.00 contact NVSL   serum
     VN (Indiana) $5.00 M, W 2-day test serum
     VN (New Jersey) $5.00 M, W 2-day test serum
West Nile Virus
     IgM capture ELISA $8.00 as needed next day serum

 
All fees subject to change without notice.