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Porcine

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Submission Fee

A $10 submission fee is assessed on each case.

Testing Schedule

Our goal is to provide accurate results and timely service. However, many diagnostic assays are not performed daily and/or testing schedules may change. If your case involves testing animals for export, testing show animals, testing animals to be sold, and/or testing a large number of samples, please contact the VDL (515-294-1950) prior to submission to verify the availability and turnaround time of specific assays. A phone call will often shorten turnaround time by allowing us time to prepare for testing prior to receipt of samples.

Fees and test schedules are subject to change without notice.

QUICK LINKS

 

Submission Guidelines

Export Testing Guidelines

User Guide

Printable Fee schedule

 

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  FEE   updated 3/28/2014
Submission fee each case $10.00     
Environmental surcharge 1% of total (not including submission fee)
Report by FAX per transmission $1.00    
Handling charge for leaky package $25.00    
Bacteria isolate 
     Slant preparation $30.00   
     Slant saved at ISU VDL $5.00  
     Slant shipped $10 - 25.00  
Shipping  
     Bacterial slant $10 - 25.00  
     Virus $75.00 - plus $30.00 if virus needs to be propagated 
     Forwarding of samples to outside lab $10 - 20.00  

 

  FEE DAYS TESTED TURN-AROUND SPECIMEN
APP - Actinobacillus pleuropneumoniae
     Bacterial serotyping $12.00      
     CF 1, 5, 7
          (no serotyping)
$5.00 W 2-day test serum
     Mix APP ELISA $12.00 M same day

serum

SEE MORE INFO

     ELISA 10-12 $4.00     serum
     PCR $25.00 M, Th    
     Serotype 1, 5, 7 $15.00 as needed   serum
     Serotype 3 $5.00 W   serum
Actinobacillus suis
     PCR $25.00 T, F same day lung
Bacterial isolation and identification   
  $25.00 1st sample
$5.00 > 1 sample
     
Bacterial susceptibility testing       
     $20.00      
Brachyspira
     Culture $15.00      
      Sequencing
       (nox gene)
$50.00 as needed 2-3 days identification from culture
     PCR $30.00 M, Th 1-2 days culture, feces
Brucella
     BAPA $1.00  daily same day serum
     Card $2.00 daily same day serum
     FPA $7.50     serum
     RAP $1.00 daily same day serum
     RIV $1.00 daily same day serum
     SPT $2.50 daily same day serum
     STT $2.50 M-W 3-day test serum
Campylobacter    
     Culture $10.00      
Clostridium difficile
     Culture $15.00      
     ELISA - toxin A/B $30.00      
Clostridium perfringens 
     PCR - toxin gene typing $30.00 M, Th same day culture
Clostridium sp.
      FA $10.00      
  COMMENTS:  includes C. novyi, chauvoei, sordelli, septicum
Coliform
     Culture $15.00     water
E. coli
     PCR - genotyping $30.00 M, Th same day culture
  Includes: STb(toxin), Sta(toxin), K99(pilus), LT(toxin), F18(pilus), 987P(pilus), K88(pilus), F41(pilus), Stx2e(toxin) genes
Encephalomyocarditis virus - EMC   
     FA $13.00 as needed same day brain, heart
     VI $25.00 as needed 7-14 days brain, heart
     VN (NVSL) $14.00 contact NVSL    
Erysipelothrix rhusiopathiae   tonsillarum & species
     ELISA $6 as needed 2 days serum
     Bacterial enrichment $20.00 as needed    
     IHC $13.00 as needed    
     PCR $30.00 M, Th next day joint fluid, skin, spleen, lung
     Serotyping $25.00 as needed 7-14 days isolates
Fungal
     Culture $25.00 1st sample
$10.00 >1 sample
   
Haemophilus parasuis  - HPS
     PCR $30.00 T, F same day lung, BAL, bronchial swab, joint fluid, pericardium (fluid/swabs), heart
Hemagglutinating encephalomyelitis virus - HEV
     FA $13.00 as needed same day brain, spinal cord
     HI (NVSL) $15.00 contact NVSL   serum 
Hepatitis E virus - HEV
     ELISA $6 based on demand 24-48 hours serum
     PCR $35.00 as needed   liver, serum, feces
Histopathology
     Biopsy $35.00      
     Histo slide $5.00      
     IHC $13.00      
     Special stains $13.00      
Influenza A      SEE MORE INFO
     AgELISA $18.00 M, W, F same day

nasal swab, tracheal wash, oropharyngeal swab

     PCR - type A
           screen and subtype
$40.00 daily same day

lung, bronchial swab, BAL, oropharyngeal swab, nasal swab, nasal washing

     PCR pH1N1 differential $25.00 daily same day lung, bronchial swab, BAL, oropharyngeal swab, nasal swab, nasal washing
     PCR - NP gene $30.00      
     PCR - HA gene $35.00      
     Sequencing $110.00      
Influenza B
     AgELISA $18.00 M,W,F same day

nasal swab, tracheal wash, oropharyngeal swab

Lawsonia intracellularis - (Ileitis)
     ELISA $8.00 as needed same day serum
     IHC $13.00      
     PCR $35.00 M, Th same day feces, fecal swab, intestinal contents
Lepto - Leptospira interrogans
     IHC $13.00 as needed    
     MAT 5 serovars $5.50  T, Th same day serum
  5 serovars = canicola, ictero, grippo, pomona, hardjo
     MAT 6 serovars $6.00  T, Th same day serum
  6 serovars = canicola, ictero, grippo, pomona, hardjo, bratislava
     PCR $30.00 T, F same day placenta, kidney, urine, semen, serum
Listeria    
     Enrichment culture $20.00      
     IHC $13.00 as needed    
Mycoplasma    
     Culture $30.00      
     Identification $25.00-35.00      
Mycoplasma hyopneumoniae
     Dako ELISA $9.50 W same day serum
     ELISA $5.50 daily same day serum
     IHC $13.00 as needed    
     PCR $25.00 daily same day lung, bronchial swab, BAL
     Tween 20 ELISA $4.75 as needed same day serum
Mycoplasma hyorhinis
     PCR $25.00 M, Th next day

joint swab or joint tissue

 

CLICK HERE TO SEE MORE INFO

Mycoplasma hyosynoviae
     PCR $25.00 M, Th next day

joint swab or joint tissue

 

CLICK HERE TO SEE MORE INFO

Mycoplasma suis - (formerly Eperythrozoon suis)
     PCR $30.00 as needed   whole blood (EDTA or heparin), liver or spleen
Necropsy    
     Necropsy

$5.00 - fetus/embryo
$10.00 - juvenile/adult

   
     Special or complex
     procedures
$60.00/hr      
Parasitology  
      Baermann

$15.00 1-5 samples
$7.50 6-10 samples
$5.00 >10 samples

M-Th   next business day
      Direct Smear $13.00 1-5 samples
$6.00 > 5 samples
daily   next business day
      Fecal flotation $15.00 1-5 samples
$8.00    >5 samples
daily   next business day
      McMaster float -
          quantitative
         
$15.00 1-5 samples
$7.50 6-10 samples
$5.00 > 10 samples
daily   next business day
      Sedimentation $15.00 1-5 samples
$8.00  > 5 samples
M-Th   next business day
  ** If submitting greater than 10 fecal samples, please contact the laboratory for scheduling, price quote, and approximately turnaround time.
Porcine circovirus type 1 - PCV1  SEE MORE EXPLANATION OF PCV PCRS
     PCR - fluid $25.00 as needed   serum, BAL
     PCR - tissue $30.00 T, F   lung, lymph nodes
Porcine circovirus type 2 - PCV2   SEE MORE INFO: PCV
     Antibody titration $15.00      serum
     ELISA $5.25 M, F same day serum
     FFN $16.00 M 3-day test serum
     Four dilution IFA $7.50      serum
     IHC $13.00 as needed    
     PCR  $30.00 daily same day serum, tissue, filter membrane (e.g. TEGO, FTA) and oral fluid
     PCR - PCV2a/b 
         multiplex differential

$25.00 serum, fluid
$30.00 tissue

as needed   serum, tissues, fluids
     Quantitation

$75.00 1st sample
$25.00 >1 sample

     
     Sequencing $150.00 as needed 5-7 days

PCR-positive sample, isolate

     VI $25.00 as needed 7-14 days

serum, lung, lymph nodes, tonsil

 

CLICK HERE TO SEE MORE INFO: PCV2

Porcine circovirus 1-2  PCV1-2
     PCR - fluid $25.00 as needed    
     PCR - tissue $30.00 as needed    
Porcine cytomegalovirus   
     PCR $35.00 T, F next day blood, nasal swab, turbinates, brain
Porcine deltacoronavirus -PDCV
     PCR $30.00 M-F same day feces, fecal swabs, intestine, oral fluids, environmental samples
Porcine enterovirus/Porcine teschovirus - PEV/PTV
     FA $13.00 as needed same day spinal cord, brain
     PCR $25.00 T, F next day spinal cord, brain
     VI $25.00 as needed 7-14 days spinal cord, brain
Porcine epidemic diarrhea  - PED - SEE MORE INFO: PED
     PCR N realtime
   
 
$30.00 daily same day feces, fecal swabs, intestine, oral fluids, environmental samples
     PCR S gel $30.00 daily same day feces, fecal swabs, intestine, oral fluids, environmental samples
     PCR nested gel $35.00 daily same day feces, fecal swabs, intestine, oral fluids, environmental samples
     IHC $13.00 as needed    
     IFA screen $5.50 as needed   serum
     IFA 4 dilution $15.00 as needed   serum
     Sequencing $150.00 as needed   feces, fecal swabs, intestine, oral fluids
Porcine epidemic diarrhea/Transmissible gastroenteritis virus - PED/TGE
     PCR
   
 
$35.00 daily same day feces, fecal swabs, intestine, oral fluids, environmental samples
Porcine parvovirus - PPV
     FA $13.00 as needed same day fetal tissues, placenta
     HI $5.25 M 24-hour test serum
     PCR $30.00 T, F next day fetal tissue
     VI $25.00 as needed 7 - 14 days serum, tissues, BAL
Porcine respiratory coronavirus - PRCV    
     IHC $13.00 as needed    
Porcine reproductive & respiratory syndrome virus - PRRSV
     ELISA X3 $5.00 M-F same day serum        SEE MORE INFO
     ELISA X3 - oral fluids $7.50 as needed next day

oral fluids   SEE MORE INFO

     FA $13.00 as needed same day lung
     FFN $15.00 M 3-day test serum
     IFA European - 
          screen @ 1:20
$5.00 M-F same day  serum
     IFA European- titration $15.00 as needed same day serum
     IFA North American -          screen @ 1:20

$5.00

as needed same day serum
     IFA North American - 
          titration
$15.00 M-F same day serum
     Homologous IFA    
         screen @ 1:20
$5.00 as needed 2-4 weeks virus culture & serum
     Homologous IFA    
         titration
$15.00 as needed 2-4 weeks virus culture & serum
     IHC $13.00 as needed    
     PCR $25.00 daily same day

serum, tissue, filter membrane (e.g. TEGO, FTA),

oral fluid
SEE MORE INFO

     Quantitative PCR $75.00 1st sample
$25.00 >1 sample
     
  $110.00  
     Sequencing $110.00 as needed    
     VI $25.00 as needed 7-14 days serum, tissues, BAL, oral fluid, blood
Porcine teschovirus - Group 1 porcine enterovirus
     PCR $25.00 T, F    
Pseudorabies - PRV
     ALA $3.00 as needed same day serum
     FA $13.00 as needed same day brain, tonsil, lung, fetal liver
     g1 ELISA $2.75 M-F same day serum
     gB ELISA (screen) $3.50 T,Th same day serum
     PCR $30.00 as needed   brain, tonsil, lung, fetal liver
     VI $25.00 as needed 7-10 days brain, tonsil, lung, fetal liver
     VN $5.00 T, F 3-day test serum
Rotavirus  
     AgELISA - type A $7.00 as needed same day feces
     IHC - type A $13.00 as needed    
     PCR - type A, B & C $30.00 M, Th next day

feces, intestinal contents, fecal swab

SEE MORE INFO

Salmonella        
     Bacterial serotyping $20.00      
     Enrichment culture $5-20.00      
Swine influenza virus - SIV - SEE MORE INFO
     FA $13.00 as needed same day lung
     H1N1 ELISA $4.75 W, F same day serum
     H1N1 Pfizer HI $5.00 T 24-hour test serum
     H1N1(99) HI $5.00 T 24-hour test serum
     H3N2 ELISA $4.75 W, F same day serum
     H3N2 HI $5.00 T 24-hour test serum
     IHC $13.00 as needed    
     NP ELISA $5.00 as needed same day serum
     VI $25.00 as needed 7-21 days lung, nasal swab, BAL, oral fluids
Transmissible gastroenteritis virus - TGE
     IHC $13.00 as needed    
     PCR $30.00 daily same day feces, fecal swab, intestinal contents
     VN $5.00 F 5-day test serum
Transmissible gastroenteritis virus/Porcine respiratory coronavirus - TGE/PRCoV
     Elisa $9.00 T, Th same day serum
Ureaplasma    
     Bacterial culture $10.00      
Vesicular stomatitis - VS
     cELISA (NVSL) $36.00 contact NVSL   serum
     CF (NVSL) $36.00 contact NVSL   serum
     VN (Indiana) $5.00 M 2-day test serum
     VN (New Jersey) $5.00 M 2-day test sesrum
West Nile Virus
     IgM capture ELISA $8.00 as needed nexxt day serum
     PCR $30.00 M, Th   serum, brain, spinal cord

 
All fees subject to change without notice.