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Pathogen

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Submission Fee

A $10 submission fee is assessed on each case.

Testing Schedule

Our goal is to provide accurate results and timely service. However, many diagnostic assays are not performed daily and/or testing schedules may change. If your case involves testing animals for export, testing show animals, testing animals to be sold, and/or testing a large number of samples, please contact the VDL (515-294-1950) prior to submission to verify the availability and turnaround time of specific assays. A phone call will often shorten turnaround time by allowing us time to prepare for testing prior to receipt of samples.

Fees and test schedules are subject to change without notice.

QUICK LINKS

Submission Guidelines

Export Guidelines

Printable Fee schedule

Submission forms

User Guide

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  FEE    updated 3/28/2014
Submission fee each case $10.00     
Environmental surcharge 1% of total (not including submission fee)
Report by FAX per transmission $1.00    
Handling charge for leaky package $25.00    
Bacteria isolate 
     Slant preparation $30.00   
     Slant saved at ISU VDL $5.00  
     Slant shipped $10 - 25.00  
Shipping  
     Bacterial slant $10 - 25.00  
     Virus $75.00 - plus $30.00 if virus needs to be propagated 
     Forwarding of samples to outside lab $10 - 20.00  

 

 

  FEE DAYS TESTED TURN-AROUND SPECIMEN
Avian influenza - AIV
     AGID - serum $2.50  M-Th 24-hour test serum
     AGID - egg yolk $2.50 M-W 48-hour test egg yolk
     ELISA $1.50 as needed same day serum
     PCR $35.00 as needed 2 days fecal, cloacal or oropharyngeal swab
     VI $50.00 as needed 7-21 days cloacal or oropharyngeal swab, feces, oral+cloacal, tracheal swab, environmental sample
Avian paramyxovirus-1 matrix gene
     PCR $30.00 as needed    
Avian pneumovirus - APV
     ELISA $1.75  as needed same day serum
Anaplasma marginale - Ana
     CARD $4.00  as needed same day serum
     CF $4.00  T, Th same day serum
     ELISA $6.00  W same day serum
     cELISA (NVSL) $18.00 contact NVSL    
Actinobacillus pleuropneumoniae - APP
     Bacterial serotyping $12.00      
     CF 1, 5, 7 
          (no serotyping)
$5.00 W 2-day test serum
     Mix APP ELISA $12.00 M same day

serum

SEE MORE INFO

     ELISA 10-12 $4.00     serum
     PCR $25.00 M, Th    
     Serotype 1, 5, 7 $15.00 as needed   serum
     Serotype 3 $5.00 W   serum
Actinobacillus suis
     PCR $25.00 T, F same day lung
Baculovirus
     ELISA $6.50 M, W, F same day serum
Bacterial isolation and identification   
  $25.00 1st sample
$5.00 > 1 sample
     
Bacterial susceptibility testing       
     $20.00      
Bluetongue - BT
     AGID $5.00  M-Th 24-hour test serum
     CF (NVSL) $18.00 contact NVSL   serum
     ELISA $8.00 F same day serum
     VN (NVSL) $14.00 contact NVSL   serum
Bovine adenovirus - BAdV
     FA $13.00 as needed same day lung
     IFA (NVSL) $15.00 contact NVSL    
     VN (NVSL) $14.00 contact NVSL    
Bovine coronavirus - BCoV    NOTE:  this test is included in the Bovine enteric panel
     FA $13.00 as needed same day lung, intestine
     IHC $13.00 as needed    
     IFA (NVSL) $15.00 contact NVSL    
     PCR $30.00 T, F same day feces, intestine
     VN (NVSL) $14.00 contact NVSL   serum

Bovine Enteric Panel - BEP

     PCR panel $50.00 daily same day

feces, intestinal content

 

COMMENTS: Includes Rotavirus group A, BCV, Salmonella spp., E coli K99, Cryptosporidium parvum

SEE MORE INFO

Bovine herpesvirus type 2   
     VN (NVSL) $14.00 contact NVSL   serum
Bovine herpesvirus type 4   
     IFA (NVSL) $15.00 contact NVSL   serum
Bovine leukemia virus - BLV
     AGID $5.00  M-F 24-hour test serum
     ELISA $5.00 F same day serum
     ELISA (NVSL) $18.00 contact NVSL   serum
Bovine parainfluenza virus type 3 - BPIV3
     FA $13.00 as needed same day lung
     VI $25.00 as needed 7-14 days lung
     VN $5.00 F 3-day test serum
Bovine respiratory syncytial virus - BRSV
     FA $13.00 as needed same day lung
     IHC $13.00      
     PCR $30.00 T, F same day lung, nasal swab
     VN $5.00 F 7-day test serum
Bovine viral diarrhea virus - BVDV  
     AgELISA - serum
                     PI test
$6.00 W, F same day serum, ear notches(fresh, not fixed)
     FA $13.00 as needed same day tissues including tonsil
     Genotyping $25.00 as needed   isolate
     PCR $30.00 T, F same day serum, fresh ear notches, blood, tissues, feces, milk, semen
     IHC - ear notches  $11.00 1-3 notches
$3.00 ea >4 notches
M-F next day

formalin-fixed ear notches

SEE MORE INFO

Use the Serum Submission Form

     VI    $25.00 as needed 7-10 days blood, tissues, serum, semen
     VN - type I $5.00 F 5-day test serum
     VN - type II $5.00 F 5-day test serum
Brachyspira    
     Culture $15.00      
     PCR $30.00 M, Th 1-2 days culture, feces
Brucella
     B. abortus/suis BAPA $1.00  M-F same day serum
     B. abortus/suis card $2.00 M-F same day serum
     B. abortus FPA $7.50     serum    SEE MORE INFO
     B. abortus/suis RAP $1.00 M-F same day serum
     B. abortus/suis RIV $1.00 M-F same day serum
     B. ovis CF (NVSL) $18.00 contact NVSL   serum
     B. ovis ELISA (NVSL) $18.00 contact NVSL   serum
     Bruc. SPT $2.50 M-F same day serum
     Bruc. STT $2.50 M-W 3-day test serum
Cache valley virus    
     VN (NVSL) $14.00 contact NVSL   serum
Campylobacter    
     Culture $10.00      
Canine adenovirus - CAdV
     FA $13.00 as needed same day tonsil, lung, liver, intestine
     VI $25.00 as needed 7-14 days tonsil, liver, kidney, splen, lung
Canine coronavirus  - CCoV
     FA $13.00 as needed same day intestine, lung
     PCR $30.00 as needed   feces, intestine
Canine distemper virus - CDV
     FA $13.00 as needed same day lung, brain
     PCR $30.00 M, Th   lung, blood, conjunctival swab, brain
Canine herpesvirus - CHV
     FA $13.00 as needed same day lung, spleen
     PCR $30.00 M, Th    
     VI $25.00 as needed 7-14 days lung, spleen, blood, corneal swab
     VN $5.00 F 3 day test serum
Canine influenza virus - CIV 
     H3N8 HI $10.00 as needed 24-hr test serum
     PCR $40.00 as needed   swabs, tracheal wash, fresh tissues
    SEE MORE INFO: influenza A screening and H3 subtyping  
     VI $25.00 as needed 7-14 days  swabs, tracheal wash, fresh tissue (lung)
Canine parvovirus - CPV
     FA $13.00 as needed same day intestine
     VI $25.00 as needed 7-14 days intestine
Caprine arthritis encephalitis virus - CAE
     AGID $5.00 M-F   serum
     cELISA $6.50 as needed same day SEE MORE INFO 
Caprine herpesvirus- CHV
     PCR $30.00 as needed    
Chlamydia psittaci
     CF (NVSL) $18.00 contact NVSL   serum
Clostridium difficile
     Culture $15.00      
     ELISA - toxin A/B $30.00      
Clostridium perfringens 

     PCR - toxin gene
              
typing

$30.00 M, Th same day culture
Clostridium sp.
      FA $10.00      
  COMMENTS:  includes C. novyi, chauvoei, sordelli, septicum
Coliform
     Culture $15.00     water
E. coli
     PCR - genotyping $30.00 M, Th same day culture
  Includes: STb(toxin), Sta(toxin), K99(pilus), LT(toxin), F18(pilus), 987P(pilus), K88(pilus), F41(pilus), Stx2e(toxin) genes
Encephalomyocarditis virus - EMC   
     FA $13.00 as needed same day brain, heart
     VI $25.00 as needed 7-14 days brain, heart
     VN (NVSL) $14.00 contact NVSL    
Epizootic hemorrhagic disease - EHD
     AGID $5.00  M-Th 24-hour test serum
     CF (NVSL) $18.00 contact NVSL   serum
     VN (NVSL) $14.00 contact NVSL   serum
Equine adenovirus 1 - EAdV1
     PCR $30.00 T, F same day nasal swab, conjunctival swab, tracheal aspirates, lung
Equine arteritis virus - EAV  
     PCR $30.00 T, F same day nasal swab, EDTA blood, lung, semen, fetal tissues (lung, liver, kidney, thymus, spleen, placenta)
     VN (NVSL) $14.00 contact NVSL   serum
Equine encephalitis virus - EEE, VEE, WEE (each virus is tested separately)
     CF (NVSL) $18.00 each (or $54.00 for all three) serum
     ELISA (NVSL) $18.00 each (or $54.00 for all three) serum
     HI (NVSL) $15.00 each (or $45.00 for all three) serum
     PCR (NVSL) $54.00  brain
     VN (NVSL) $14.00 each (or $42.00 for all three) serum
Equine herpesvirus type 1 (Eq. rhino.) - EHV1
     FA $13.00 as needed same day fetal tissues (lung, liver, thymus, spleen, placenta), lung
     PCR-neuropathotyping $35.00 T, F same day nasal swab, EDTA blood, lung, brain, cerebral spinal fluid, fetal tissues (lung, liver, kidney, thymus, spleen, placenta)
     VI $25.00 as needed 7-14 days fetal tissues (lung, liver, thymus, spleen, placenta), lung, blood, nasal swab
     VN (NVSL) $14.00 contact NVSL   serum
Equine herpesvirus type 1&4 - EHV1&4
     PCR $35.00 T, F same day nasal swab, EDTA blood, lung, brain, cerebral spinal fluid, fetal tissues (lung, liver, kidney, thymus, spleen, placenta)
Equine herpesvirus type 2 - EHV2
     PCR $30.00 T, F same day nasal swab, conjunctival swab, tracheal aspirates, lung
Equine herpesvirus type 3 - EHV3
     FA $13.00 as needed same day lung, spleen, lymph nodes
     PCR $30.00 T, F $30.00 swab/scraping from reproductive tract
     VI $25.00 as needed 7-14 days fetal tissues, lung, blood
     VN (NVSL) $14.00 contact NVSL   serum
Equine herpesvirus type 4 - EHV4
     FA $13.00 as needed same day fetal tissues (lung, liver, thymus, spleen, placenta), lung 
     VI $25.00 as needed 7-14 days fetal tissues (lung, liver, thymus, spleen, placenta), lung, blood, nasal swab
     VN (NVSL) $14.00 contact NVSL   serum
Equine infectious anemia - EIA
     AGID $6.00  M-F 24-hour test serum
     ELISA $6.00  M-F same day serum
Equine influenza virus - EIV
     HI - Kentucky
        
(NVSL)
$15.00 contact NVSL   serum
     HI - Miami (NVSL) $15.00 contact NVSL   serum
     PCR $30.00 T, F same day nasal swab, tracheal washings, lung
     VI $25.00 as needed 7-14 days swabs, tracheal wash, fresh tissues
Equine rhinitis A and B virus - ERAV & ERBV
     PCR $35.00 T, F same day nasal swab, conjunctival swab, tracheal aspirates, urine
Erysipelothrix rhusiopathiae  tonsillarum & species
     Bacterial enrichment $20.00      
     ELISA $6 as needed 2 days serum
     IHC $13.00 as needed    
     PCR $30.00 M, Th next day joint fluid, skin, spleen, lung
     Serotyping $25.00 as needed 7-14 days isolates
Feline calicivirus - FCV
     FA $13.00 as needed same day lung
     VI $25.00 as needed 7-14 days lung, nasal or tracheal swab
Feline coronavirus - FCoV   
     FA $13.00 as needed same day tissues including lung, liver, spleen, lymph nodes, intestine
Feline herpesvirus - FHV
     FA $13.00 as needed same day lung
     VI $25.00 as needed 7-14 days lung, nasal or tracheal swab
Feline parvovirus - FPV
     FA $13.00 as needed same day blood tissues
     VI $25.00 as needed 7-14 days blood tissues
Fungal
     Culture $25.00 1st sample
$10.00 >1 sample
   
Haemophilus parasuis  - HPS
     PCR $30.00 T, F same day lung, BAL, bronchial swab, joint fluid, pericardium (fluid/swabs), heart
Haemophilus somnus - HS
     CF $5.00 T, Th same day serum
Hemagglutinating encephalomyelitis virus - HEV
     FA $13.00 as needed same day brain, spinal cord
     HI (NVSL) $15.00 contact NVSL   serum 
Hepatitis E virus - HEV
     ELISA $6 based on demand   serum
     PCR $35.00 as needed   liver, serum, feces
Histopathology
     Biopsy $20.00      
     Histo slide $5.00      
     Special stains $13.00      
Infectious bovine rhinotracheitis virus - IBRV
     FA $13.00 as needed same day lung, lymph nodes, spleen
     IHC $13.00 as needed    
     PCR $30.00 T, F same day  
     VN $5.00  F 3-day test serum
     VI $25.00 as needed 7-14 days lung, lymph nodes, blooe
Influenza A virus
     AgELISA $18.00 M, W, F same day nasal swab, tracheal wash, oropharyngeal swab
     $30.00      
     PCR - screen and
               subtype
$40.00 daily same day

lung, nasal swab, BAL, oral fluids

     PCR - NP gene $30.00      
     PCR - HA gene $35.00      
     Sequencing $110.00      
Influenza B virus        
     AgELISA $18.00 M, W, F same day nasal swab, tracheal wash, oropharyngeal swab
Johne's - Mycobacterium paratuberculosis
     AGID $5.00 M 24-hour test serum
     CF $5.00 F same day serum
     ELISA $5.00 W, F same day serum, plasma, milk
     Liquid culture $25.00 single
            sample
$27.00 pooled
     
     PCR $30.00 single
            sample
$35.00 pooled
M, Th same day feces
  SEE JOHNE'S INFO    
Lawsonia intracellularis - (Ileitis)
     ELISA $8.00 as needed same day serum
     IHC $13.00      
     PCR $35.00 M, Th same day feces, fecal swab, intestinal contents
Lepto - Leptospira interrogans
     IHC $13.00 as needed    
     MAT 5 serovars $5.50  Th same day serum
  5 serovars = canicola, ictero, grippo, pomona, hardjo
     MAT 6 serovars $6.00  Th same day serum
  6 serovars = canicola, ictero, grippo, pomona, hardjo, bratislava
     PCR $30.00 T, F same day placenta, kidney, urine, semen, serum
Listeria    
     Enrichment culture $20.00      
     IHC $13.00 as needed    
Malignant catarrhal fever - MCF
     IPT (NVSL) $16.00 contact NVSL    
     PCR $30.00 M, Th    
     VN (NVSL) $14.00 contact NVSL    
Mycobacterium paratuberculosis - see Johne's
Mycoplasma    
     Culture $30.00      
     Identification $25.00-35.00      
Mycoplamsa bovis    
     IHC $13.00      
     PCR $25.00 T, F   lung, BAL, milk, joint fluid
Mycoplasma hyopneumoniae
     Dako ELISA $9.50 W same day serum
     ELISA $5.50 M-F same day serum
     IHC $13.00 as needed    
     PCR $25.00 daily same day lung, bronchial swab, BAL
     Tween 20 ELISA $4.75 as needed same day serum
Mycoplasma hyorhinis
     PCR $25.00 M, Th next day

joint swab or joint tissue

 

SEE MORE INFO

Mycoplasma hyosynoviae
     PCR $25.00 M, Th next day

joint swab or joint tissue

 

SEE MORE INFO

     
Mycoplasma suis - (formerly Eperythrozoon suis)
     PCR $30.00 as needed   whole blood (EDTA or heparin), liver or spleen
Necropsy    
 

$5.00 - fetus/embryo
$10.00 - juvenile/adult

   
     Special or complex
     procedures
$60.00/hr      
Neospora     
     ELISA $6.00 F same day serum
     IHC $13.00 as needed    
Ovine progressive pneumonia - OPP
     AGID $5.00 M same day serum
Parasitology  
      Baermann

$15.00 1-5 samples
$7.50 6-10 samples
$5.00 >10 samples

M-Th   next business day
      Direct Smear $13.00 1-5 samples
$6.00 > 5 samples
daily   next business day
      Fecal flotation $15.00 1-5 samples
$8.00    >5 samples
daily   next business day
      McMaster float -
          quantitative
         
$15.00 1-5 samples
$7.50 6-10 samples
$5.00 > 10 samples
daily   next business day
      Sedimentation $15.00 1-5 samples
$8.00  > 5 samples
M-Th   next business day
  ** If submitting greater than 10 fecal samples, please contact the laboratory for scheduling, price quote, and approximately turnaround time.
Pasteurella multocida - toxin gene
     PCR $25.00 M, Th same day culture
Porcine circovirus - PCV   SEE MORE EXPLANATION OF PCV PCRS
     PCR - fluid $25.00      
     PCR - tissue $30.00      
Porcine circovirus type 1 - PCV1
     PCR - fluid $25.00 as needed   serum, BAL
     PCR - tissue $30.00 as needed   lung, lymph nodes
Porcine circovirus type 2 - PCV2   SEE MORE INFO: PCV
     Antibody titration $15.00      serum
     ELISA $5.25 W, F same day serum
     FFN $16.00 M 3-day test serum
     Four dilution IFA $7.50      serum
     IHC $13.00 as needed    
     PCR - fluid $25.00 daily same day fluid
     PCR - tissue $30.00 daily same day tissue, filter membrane (e.g. TEGO, FTA),
     PCR - oral fluid $30.00 daily next day oral fluid
     PCR - PCV2a/b 
         multiplex differential

$25.00 serum, fluid
$30.00 tissue

as needed   serum, tissue, fluid
     Quantitation

$75.00 1st sample
$25.00 >1 sample

     
     Sequencing $150.00 as needed 5-7 days

PCR-positive sample, isolate

     Virus isolation $25.00 as needed 7-14 days

serum, lung, lymph nodes, tonsil

 

SEE MORE INFO: PCV2

Porcine circovirus 1-2   PCV1-2   
     PCR - fluid $25.00 as needed    
     PCR - tissue $30.00 as needed    
Porcine cytomegalovirus   
     PCR $35.00 T, F next day blood, nasal swab, turbinates, brain
Porcine enterovirus & Porcine teschovirus  - PEV & PTV
     FA $13.00 as needed next day spinal cord, brain
     PCR $25.00 T, F    
     VI $25.00 as needed 7-14 days spinal cord, brain
Porcine parvovirus - PPV
     FA $13.00 as needed same day fetal tissues, placenta
     HI $5.25 M 24-hour test serum
     IHC $13.00      
     PCR $30.00 t, F next day fetal tissue
     VI $25.00 as needed 7 - 14 days serum, tissues, BAL
Porcine respiratory coronavirus - PRCV    
     FA $13.00 as needed same day lung
     IHC $13.00 as needed    
Porcine reproductive & respiratory syndrome virus - PRRSV
     ELISA X3 $5.00 M-F same day serum       SEE MORE INFO
     ELISA X3 - oral fluids $7.50 as needed next day

oral fluids

  SEE MORE INFO on oral fluids
     FA $13.00 as needed same day lung
     FFN $15.00 M 3-day test serum
     IFA European - 
          screen @ 1:20
$5.00 as needed same day  serum
     IFA European -
          titration
$15.00 as needed same day serum
     IFA North American -          screen @ 1:20

$5.00

M-F same day serum
     IFA North American - 
          titration
$15.00 M-F same day serum
     Homologous IFA    
         screen @ 1:20
$5.00 as needed 2-4 weeks virus culture & serum
     Homologous IFA    
         titration
$15.00 as needed 2-4 weeks virus culture & serum
     IHC $13.00 as needed    
     PCR $25.00 daily same day serum, tissue, oral fluid, filter membrane (e.g. TEGO, FTA),
     Quantitative PCR $75.00 1st sample
$25.00 >1 sample
     
     Sequencing $110.00      
     VI $25.00 as needed 7-14 days serum, tissues, BAL
Porcine teschovirus - Group 1 porcine enterovirus
     PCR $25.00 T, F    
Pseudorabies - PRV
     ALA $3.00 as needed same day serum
     FA $13.00 as needed same day brain, tonsil, lung, fetal liver
     g1 ELISA $2.75 M-F same day serum
     gB ELISA (screen) $3.50 T, Th same day serum
     PCR $30.00 as needed   brain, tonsil, lung, fetal liver
     VI $25.00 as needed 7-14 days fetal tissue
     VN $5.00 T, F 3-day test serum
Pullorum
     AGG $3.00 as needed same day serum
Q Fever (Coxiella burnetii)
     CF (NVSL) $18.00 contact NVSL   serum
Rabies    
     FA $30.00 daily same day brain
     Emergency -  
        weekend
        and after-hours
$130.00 as needed same day brain
Rotavirus - type A    
     AgELISA $7.00 as needed same day feces
     IHC $13.00 as needed    
     PCR - type A, B & C $30.00 M, Th next day

feces, intestinal contents, fecal swab
SEE MORE INFO

Salmonella    
 Bacterial serogrouping $20.00 as needed    
     Enrichment culture $5-20.00      
Streptococcus suis    
     Bacterial serotyping $20.00 as needed    
     IHC $13.00 as needed    
Swine influenza virus - SIV - SEE DISEASE INFO
     FA $13.00 as needed same day lung
     H1N1 ELISA $4.75 W, F same day serum
     H1N1 Pfizer HI $5.00 T 24-hour test serum
     H1N1(99) HI $5.00 T 24-hour test serum
     H3N2 ELISA $4.75 W, F same day serum
     H3N2 HI $5.00 T 24-hour test serum
     IHC $13.00 as needed    
     NP ELISA $5.00 as needed same day serum
     VI $25.00 as needed 7-21 days lung, nasal swab, BAL, oral fluids
Toxo - Toxoplasma gondii
     IHC $13.00 as needed    
     LAT $5.00 M-Th next day serum
Transmissible gastroenteritis virus - TGE
     IHC $13.00 as needed    
     PCR $30.00 daily same day feces, fecal swab, intestinal contents
     VN $5.00 F 5-day test serum
Transmissible gastroenteritis virus/Porcine respiratory coronavirus - TGEV/PRCoV
     ELISA $9.00 T, Th same day serum
     FA $13.00 as needed same day lung (PRCoV)
Trichomonas
     Bacterial culture $7.00      
Tritrichomonas foetus 
     PCR $25.00 daily same day

bovine & feline-feces only

inoculated trich pouch (InPouch), preputial wash

  SEE MORE INFO  
Ureaplasma    
     Bacterial culture $10.00      
Vesicular stomatitis - VS
     cELISA (NVSL) $36.00 contact NVSL   serum
     CF (NVSL) $36.00 contact NVSL   serum
     VN (Indiana) $5.00 M 2-day test serum
     VN (New Jersey) $5.00 M 2-day test serum
Virus quantitation
  $120.00      
West Nile Virus
     IgM capture ELISA $8.00 as needed nest day serum
     PCR $30.00 M, Th   serum, brain, spinal cord
     PCR (NVSL) $54.00 contact NVSL brain

 

All fees subject to change without notice.