Our laboratory has characterized a pattern of intracellular survival for Brucella canis that varies depending on the host species of the monocytes. These changes in replication and survival rates correspond with the relative virulence of B. canis in these host species. Identifying the mechanisms responsible for host speciation of intracellular survival of this species may uncover host specific pathogenic events of the human pathogenic species of B. melitensis, B. abortus and B. suis.
Our lab has undertaken a collaborative project with Dr. Balaji Narisimhan to examine the cellular distribution of polyanhydride nanospheres used to formulate improved subunit vaccines for delivery to humans and animals. We demonstrated that such particles are delivered to organized, intracellular structures that are diverse in their cellular function. We are currently working towards understanding the relative contribution each of these varied intracellular compartments makes towards the unique and highly desirable adjuvanticity characteristics of the polyanhydrides copolymers.