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Diagnostic Submission

Appropriate Diagnostic Submissions for PCV2-Associated Diseases

Because PCV2 infection is often multisystemic in growing pigs and because identification of coinfections is important in the prevention and control of PCV2-associated disease, a diagnostic investigation of cases suspected to be any of the PCV2-associated disease should include a full set of tissues from affected pigs.  We recommend working up all cases as if you suspect PCV2-associated severe systemic disease.

Severe systemic infection of PCV2 (formerly known as PMWS).  Submissions to confirm systemic infection should include the following:

  • fresh and formalin fixed tonsil
  • fresh and formalin fixed spleen
  • 4-6 fresh and formalin-fixed lymph nodes (preferably inguinal, iliac, mesenteric, tracheobronchial and any others that are enlarged)
  • fresh and formalin-fixed lung
  • fresh and formalin-fixed liver
  • fresh and formalin-fixed kidney
  • fresh and formalin-fixed ileum
  • fresh and formalin-fixed colon
  • fresh and formalin fixed skin from lesions if they exist on the body, legs  or ears
  • serum for serology and PCR

PCV2-associated pneumonia.  Submissions to confirm PCV2-associated pneumonia should include the following:

  • fresh and formalin-fixed lung
  • serum for serology and PCR

PCV2-associated enteritis. Submissions to confirm PCV2-associated pneumonia should include the following:

  • fresh and formalin-fixed ileum and colon
  • serum for serology and PCR

PCV2-associated porcine dermatitis and nephropathy syndrome (PDNS).  Submissions to confirm a diagnosis of PDNS should include the following:

  • fresh and formalin-fixed skin
  • fresh and formalin fixed kidney
  • serum for serology and PCR

PCV2-associated reproductive failure.  Submissions to confirm PCV2-associated reproductive failure should include the following:

  • fresh, chilled whole fetuses
  • serum from affected and unaffected sows and boars for serology and PCR

Swine Postmortem Techniques – A Virtual Wet Lab

DIAGNOSIS

Clinical Manifestiation and Diagnosis of PCVAD

PCV2 Associated Lesions

Available Tests

PCV2

General Info

Research

Control

Diagnosis

Factors

PCVAD

Molecular Organization

Pathogenesis

Host Range

Epidemiology

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