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Dr. Tanja Opriessnig - Diagnostic Tests

Home  Lab Group Announcements Diagnostic Tests PCV2

Diagnostic tests currently under evaluation and used in our Research Laboratory:

All tests listed are available upon request and pre-arrangement. Please contact the VDL (515-294-1950) for further information. 
 

Swine HEV: Quantitative RT real-time-PCR
Preferred samples: Serum, liver, bile, feces
Storage conditions: Fresh (+4°C) or ultra-low (-80°C)

PCV2a and PCV2b: Quantitative differential real-time PCR
Preferred samples: Serum, tissues (lungs, lymphoid tissues)
Storing conditions: Fresh (+4°C) or frozen (-20°C)

PCV2 DNA in semen: Quantitative real-time PCR (+internal positive control)
Preferred samples: Semen
Storage conditions: Fresh (+4°C) or frozen (-20°C)

Chimeric PCV1-2: Real-time PCR
Preferred samples: Serum, tissues (lungs, lymphoid tissues)
Storage conditions: Fresh (+4°C) or frozen (-20°C)

Porcine parvovirus (PPV): Quantitative real-time PCR
Preferred samples: Serum, lymphoid tissues, heart (fetuses)
Storage conditions: Fresh (+4°C) or frozen (-20°C)

Erysipelothrix spp. differential real-time PCR: Specific for E. rhusiopathiae, E. tonsillarum, and E. sp. strain 2
Preferred samples: Oral fluid, porcine tissues such as skin lesions, liver, spleen, lungs, bacterial isolates
Storage conditions: Fresh (+4°C) or frozen (-20°C)

Erysipelothrix spp. spa A, B, and C differential real-time PCR: Specific for the structural proteins (spa) A, B, and C
Preferred samples: Oral fluid, porcine tissues such as skin lesions, liver, spleen, lungs, bacterial isolates
Storage conditions: Fresh (+4°C) or frozen (-20°C)

Porcine retroviruses A/B/C: RT real-time-PCR
Preferred samples: Serum, tissues
Storage conditions: Fresh (+4°C) or ultra-low (-80°C)

Porcine retroviruses A/C recombinants: Quantiative RT real-time-PCR
Preferred samples: Serum, tissues
Storage conditions: Fresh (+4°C) or ultra-low (-80°C)

Porcine retrovirus C: RT real-time-PCR
Preferred samples: Serum, tissues
Storage conditions: Fresh (+4°C) or ultra-low (-80°C)

Bungowannah virus: RT real-time-PCR
Preferred samples: Serum, tissues (hearts, etc)
Storage conditions: Fresh (+4°C) or ultra-low (-80°C)