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July 2010

 

July 27, 2010

Welcome!

Over the past 10 years, the College of Veterinary Medicine has evolved from a primary research focus in production animal medicine to one that includes excellence in the use of animal models to develop understanding of human disease and the human/animal interface that is critical to the transmission of many diseases currently receiving international attention. We have aggressively developed a team of scientists that is developing highly competitive, biomedical research programs to examine the basis of human diseases, their prevention and treatment, and support what is truly becoming “One Health/One Medicine.”

On September 15, 2010, the college will help host the first “One Health Symposium: People, Plants & Animals” at the Scheman Building. This all-university event will feature two exciting keynote speakers, four work sessions and a poster session. You’ll find more details later in this newsletter. We hope you all will participate!

This inaugural edition of CVM Research Insight provides a variety of information about the exciting discovery taking place at the College of Veterinary Medicine, the people who are doing it, and the facilities and equipment that are making it possible. Some lengthier stories are summarized in the following e-mail and links are provided to provide more complete information.

As in any discovery, you won’t find all the answers to your questions in one sitting. Our hope is that this quick look at current research activities will cause you to think, “What next?” from the research team at Iowa State.

Sincerely,

Dr. Lisa K. Nolan
Associate Dean, Research and Graduate Studies

 


 

Upcoming Events

August 17 Research Day
September 1 Faculty-Staff College Convocation
September 15 One Health Symposium: People, Plants and Animals (Save the date!)

 


  

Save The Date! September 15 One Health Symposium

The first campus-wide symposium to discuss the interconnectedness of human, animal, plant, and environmental health will be held in the Scheman Building from 7:30 a.m. to 6:00 p.m. on September 15. Dr. Roger Mahr, chief executive officer of the One Health Commission, and Dr. Josephine Briggs, director of the NIH National Center for Complementary and Alternative Medicine will be featured speakers. If you have any interests in translational research and NIH programs, this symposium is a must!

 


 

Proof-of-Concept Awards Available through CAHDIT

Proposals are being requested for seed grant money that will encourage and facilitate transfer of discovery here in the College of Veterinary Medicine into commercially viable products or processes. The grant initiative is being offered by the Center for Advanced Host Defense Immunobiotics and Translational Comparative Medicine (CAHDIT) and is administered through the Associate Dean of Research and Graduate Studies.

This unique program is available to any CVM faculty member or technician who has a qualified proof-of-concept project that fulfills program criteria. Award amounts will generally be from $10,000 to $15,000, but could be more depending on the project. An idea must pass a due diligence process and have a clearly demonstrable commercial potential. The center will receive applications and evaluate their potential commercial success. Regardless of the decision, professional faculty from CAHDIT’s translational comparative medicine section will review the principal investigator’s/ inventor’s project to recommend and suggest further pathways for development.

While submissions are open year round, there is a limit of up to ten awards that may be made, so timeliness is important.

Find out more. Details of the announcement and process are available at http://vetmed.iastate.edu/announcements. More information regarding the initiative can be found at http://vetmed.iastate.edu/sites/default/files/centers-institutes/cahdit/iowareport.pdf.

 


 

New qPCR Assay Consultation Services

Generate accurate and precise qPCR data the very first time - regardless of prior experience is the promise
Jack Gallup, VetPath, is offering with on-site and remote consultation for comprehensive qPCR assay design. General services include:

  • Basic information/qPCR theory and math
  • Primer-probe design assistance
  • One- or Two-Step systems: identifying appropriate Master Mixes and platforms
  • MIQE-based RNA isolation, DNAse and RT suggestions & guidance
  • Nucleic acid concentration measurement consultation
  • Processing of sample readings using novel custom ISU software (endorsed by
  • Dr. Stephen A. Bustin)
  • Avoidance of RT and PCR inhibition among all sample types and isolation methods
  • File system creation and initial Test Plate set-up printouts and consultation
  • Processing of Test Plate results into final set-up parameters and printouts
  • Custom Excel spreadsheets for final E-corrected data analysis and graphing
  • All steps printed out as easy-to-follow recipes for immediate in-lab use

Contact Jack at 294-5844 or 291-3696, or by email: eag@iastate.edu.

 


 

Grant Doctor

Have questions regarding improving your grantsmanship? The CVM has a number of highly successful researchers who have been highly successful in applying for and getting key grant awards. If you would like to improve your grant proposal skills, ask the Grant Doctor by sending an e-mail with your questions to cvmres@iastate.edu. Your issues will be addressed by our grant advisory team.

 


 

Building 46 Has Arrived!

Modular Research Building: The new research building (VMRI 46) was delivered and installed the week of July 12. Work on the heating, ventilating and air conditioning system and interior finishing are underway now. The facility should be operational this fall.

 

 

 

 

 

 

 

 

 


 

Brief Bytes

Picture Perfect: Two former VMRI members were featured on the cover of the journal Avian Diseases. Drs. H.E. Biester and L.H. Schwarte were the original editors of Diseases of Poultry and edited the publication for five editions starting in 1943.

Fort Dodge Animal Health Fellowships Announced: Fort Dodge Animal Health/Pfizer has announced the fellowship recipients for 2010/2011. Eric Burrough a PhD student in Veterinary Pathology, and Tara Grinnage-Pulley, a PhD student in Veterinary Microbiology. Mentors are Dr. Michael Yeager and Dr. Qijing Zhang, respectively.

Success Continues to Build: While the CVM has consistently ranked between first and third in research funded by the U.S. Department of Agriculture, funding from the National Institutes of Health has grown 20-fold in recent years. The college and its researchers are positioned well to be major contributors to growing discoveries in the field of translational medicine.

Collaborative Effort Starts with U of Iowa Pharmacy School: The College hosted the new dean of the University College of Pharmacy, Donald Letendre and colleagues on April 4 to explore collaborative research opportunities. Each department presented an overview of research strengths and potential targets for collaboration. Presenters included Drs. Bloedel, Kanthasamy, Carlson, R. Martin, Nara, Andreasen, Ackermann, Hostetter, Jones, C. Petersen, Bagley, K. Kraus, Sponseller, S. Martin, Minion, Halbur, Millman and Frana. The next steps in this process are pending.

 


 

Changing Faces

New Researchers

Dr. Alexander Krebs, VCS
received his DVM from Colorado State in 2004, completed an internship at Illinois in 2005 and a residency in small animal surgery at Iowa State. Research interests include:

Dr. Kevin Esch, VetPath Adjunct Instructor,
BS in Animal Science and DVM from Iowa State. Currently is a PhD student in interdepartmental Immunobiology. His research focus is canine visceral leishmaniasis.

 

 

 

Dr. Glenn Songer, Ph.D, VMPM
with primary research interest in Clostridium perfringens. He received his Ph.D. in Veterinary Microbiology from Iowa State.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

  

 

 

 

 

Retirements

  Ms. Sevinc Akinc
retired on June 1. She joined the college in 1985 as a lab assistant and was serving as a Program Assistant in the Office of Research and Graduate Studies when she retired.

 

 

 

 

 

 

 

 

  


 

Advice from an Insightful Mentor: This issue, Dr. Anumantha Kanthasamy

When asked to write a “what new researchers need to know” article, I was tempted to begin with a list of essential personality traits that distinguish researchers from other professionals: critical thinking, technical skills, curiosity, enthusiasm and, perhaps the most important of all, perseverance. Researchers in it for the long haul have to have exceptional perseverance. But then I realized anyone working in a fine research institution like Iowa State would already exemplify all of these qualities. Listing successful personality traits of successful researchers would be stating the obvious for people already excelling.

So I opted to turn introspective. I began by thinking about things that have made a difference in my own career and then made a list of suggestions that I offer to the newest among us doing research here in the College of Vet Med:

  1. Find a research area that you are passionate about.
    Scientists too often tailor their work to match funding and then miss their real passions. Don’t chase the dollar signs. Find a project you can put your heart into. Begin by understanding the current state of knowledge in your area, then identify major knowledge gaps and innovate to the point that when you explain your research to other experts in the field, they say ‘What a cool idea.'
     
  2. Be Innovative but keep precision in all things.
    On any task, precision determines if you will get it right the first time. Not only does precision need to be a personal quality, it also should be central to the way your work is organized. A precise organizational system is a frame work on which everything else in your career can grow and expand. By comparison, disorder grows exponentially until you are hamstrung by the creative chaos that surrounds you. Precision and an orderly frame work will support you in all areas of your career.
     
  3. Keep your focus.
    The need to stay organized is directly followed by the need to stay focused. The great writer William Shakespeare said: “Nothing comes from nothing,” which also is true if you substitute “Nothing comes from too much everything.” It’s important to be selective about your choices and the directions you move in. Too often, early stage investigators propose too much in grant applications. Nobody can do it all. Tangents can leave you fragmented and distinguished at nothing in particular. While it’s essential to seek out new opportunities, be sure to focus on your strengths and know your limits before opening new doors.
     
  4. Embrace collaboration, don’t fear it.
    When it comes to securing funding, it’s a tough world out there and it’s only getting worse. While the temptation to play your cards close to your chest is only natural, funding agencies want collaborative efforts and working with a Co-P.I. will enhance your chances for an award. The synergism of a good collaboration brings new energy and insights to any project. As a practical issue, additional author names also increase the likelihood that results of your research will be located on literature reviews and used by others.
     
  5. Publish and Network.
    These two tasks can seem to intrude on the orderly flow of any research project. Time is precious. Not only is there never enough of it, but time-sensitive tasks can dictate rigid lab schedules. Success for any researcher comes not only from results but also from reputation, and building one takes time. Never underestimate the value of getting your work out in front of others whether by writing, speaking or interacting with others at professional gatherings. And don’t wait for the big conclusion to write about your work. Write sequentially with each finding of importance. Submit, then if you need to, shrug off rejection and submit again.
     
  6. Work hard and don’t be complacent.
    Don’t sit back admiring your accomplishments. Enjoy your success for a couple of days and then move on to crunching out the work. Hard work is the secret ingredient that moves a researcher from good to extraordinary. Long hours may demand personal sacrifice. But a life dedicated to research and discovery is also exhilarating like no other. At the end of your career you’ll be able to say, “I made a difference in the world.” Who can ask for more than that?

 


 

Researcher Highlights

What are you doing? Why is it important? Why are you excited about it? What do you hope to accomplish? These are the questions we are asking CVM researchers about their research programs. Here is a sampling.

Dr. Brett Sponseller, VCS, NIH Project Award
What will the award enable you to do?
Chronicle the evolution of the cytokine milieu and oxidative capacity of pulmonary alveolar and tissue macrophages from the neonatal period through adulthood. Reactive intermediates of oxygen and nitrogen have a broad spectrum of activity against intracellular pathogens, in particular those that frequently cause severe pneumonia and death in young foals and children.
Why are you excited about what you are doing?
The study results are expected to provide the knowledge needed to (A) Develop diagnostic assays that predict an individual neonate’s susceptibility to pneumonia, and (B) Test pharmacologic and immunotherapeutic strategies to specifically regulate the cytokine and oxidative effector function of macrophages during the neonatal period.
What do you hope to accomplish with this research?
We hope this research will enable us to develop approaches that reduce the severity and frequency of pulmonary infections in young animals and children. Pneumonia is the leading cause of death of children in the world today; it is also the leading cause of death in young foals.

Diana Peterson, BMS, NIH R03
What will the award enable you to do?
The award has enabled the hiring of technician and student support for the next three years and the purchase of a Zeiss Axiolmager microscope that will be used by numerous labs to perform extensive anatomical, morphological, and stereological analysis in bith 2D and 3D.
Why are you excited about what you are doing?
I am interested in understanding how the brain works. I examine what makes sensory stimuli significant and how the brain uses that information to optimize its reactions. This research gives us better insight into how animals and humans are able to perceive their world, learn from their environment, and alter subsequent behavior based on their experience. When working correctly, these brain circuits enable us to live and behave appropriately. Although being able to understand and react appropriately to your surroundings is essential to the survival and wellbeing of both animals and humans, surprisingly little is known about how the brain is able to perform these complex functions.
What do you hope to accomplish with this research?
This research will give us a better understanding of how we are able to learn from our experience and behave appropriately in any given situation. These studies will provide the groundwork for subsequent studies on pathological conditions such as schizophrenia, bipolar disorder, attention deficit disorder, addiction and depression in which one or more of these brain circuits are performing abnormally. These current studies may be instrumental in structuring our understanding of these diseases and therefore are necessary first steps in finding potential therapies and cures.

Chris Minion and Mike Wannemuehler, VMPM, Office of Naval Research
What will the award enable you to do?
The funding will allow us to continue our development of a vaccine delivery system based on nanoparticles and identify candidate antigens of Yersinia pestis.
Why are you excited about what you are doing?
The vaccine delivery system is novel and shows promise as an excellent way to introduce one application vaccines in animals as well as human populations in third world countries where vaccine stability is a problem and where compliance is also nonexistent.
What do you hope to accomplish with this research?
Our ultimate goal is to develop an improved vaccine against Yersinia pestis, but the delivery system should be applicable to many pathogens and situations.

Richard Martin, BMS, NIH
What will the award enable you to do?
Nematode parasites cause massive morbidity affecting more than 1.4 billion people worldwide. These are known as the neglected tropical diseases (NTAs) because they have been largely been ignored, unlike turberculosis, HIV/AIDS and malaria. Rather than death, NTAs (ascariasis, elephantiasis, trichuriasis and hookworm) cause debility, poor growth, loss of productivity and poverty.
Drugs for treating human menatode parasites, anthelmintics like pyrantel, levamisole, ivermectin, were mostly developed for animal use because of the economics. However, continued use leads to the development of resistance in human and animal parasites. We are exploring the mechanism of action and resistance of cholinergic drugs in Ascaris suum, Oesophagostomum dentatum and C. elegans using patch-clamp and molecular techniques.
We are able to study mechanisms of drug resistancve and to contribute to the discovery of a novel ‘resistance busting’ anthelmintic. Derquantel is one example.

Why are you excited about what you are doing?
It is the interplay of a number of important biological concepts, some of which are still being discovered. Biology uses many undiscovered tricks that allow different species to survive. The lowly worm (the nematode) is not simple, although it is very ancient. Because its genome is bigger than that of bacteria, nematode parasites can play different tricks to permit survival. The worms have a nervous system and this can be modified to develop drug resistance.

The discovery of mechanisms of parasite drug resistance and synthesis of drugs to treat diseases dates back to Paul Ehrlich (1854-1914), the father of modern chemotherapy. It is the idea of curing disease by finding a ‘magic bullet’ to cure diseases that is very appealing.

What do you hope to accomplish with this research?
In the simplest terms, make a significant contribution to our understanding of drug resistance in parasites and counter the problem of drug resistance.

Dr. James Roth, USDA/ARS/NADC
What will the award enable you to do?
This funding will be used to develop serological assays that can be used for pandemic H1N1 surveillance in swine and to evaluate cross protective cell-mediated immunity in to different strains of swine influenza viruses. We will conduct studies to determine the pathogenesis and transmissibility of selected isolates of the wild-type novel A/H1N1 virus from animals and correlate with genetic and antigenic changes.
Why are you excited about what you are doing?
The research will be conducted in collaboration with scientists at the USDA ARS National Animal Disease Center and will help to strengthen collaborative research between NADC and the ISU College of Veterinary Medicine.
What do you hope to accomplish with this research?
We will evaluate selected antigenic epitopes and genetic factors of swine influenza virus that could lead to heterologous immunity. We hope to develop improved vaccines that have broader protective immunity against novel emerging influenza strains.

 


 

Research Advisory Committee Guides CVM Research Agenda

As in many good organizations, a lot of the real work goes on behind the scenes. Such is the case with the CVM Research Advisory Committee. Fifteen members, representing all research areas of the college, are charged with:

  • Assisting in developing faculty research program;
  • Reviewing and making recommendations on intramural research program funding requests;
  • Monitoring and assisting with research progress reports to various constituencies;
  • Assisting faculty with grantsmanship;
  • Addressing issues and concerns;
  • Formulating research-related missions, goals and objectives fostering NADC/NVSL and ISU-CVM collaborations;
  • Providing an annual report to the dean of the college.
  • Advising the Associate Dean for Research and Graduate Studies

Chaired by Dr. Lisa Nolan, Associate Dean for Research and Graduate Studies, the committee reviews a wide range of research-related programs, policies and opportunities to ensure the college is in compliance with regulations and has the resources needed to take advantage of funding opportunities as they arise. Members of the council include doctors:

Jeff Beetham Al Jergens Annette O’Connor
Vlastislav Bracha Arthi Kanthasamy Tanja Opriessnig
Steve Carlson Suzanne Millman Brett Sponseller
Nancy Cornick Chris Minion Charles Thoen
Jesse Hostetter Lisa Nolan David Wong

 

 

 

 

 


 

Research Awards February/March/April/May 2010

More than 50 researchers were identified as PIs or Co-PIs who were participating in awards totaling more than $6.7 million in February, March, April and May of this year. Programs have also been funded through the Healthy Livestock Initiative (HLI) and Iowa Livestock Health Advisory Council. Congratulations to all! 

Brett Sponseller (PI)
Mark Ackermann (Co-PI)

Development of Pulmonary Immunocompetency: Focus on the Neonate
Sponsor: NIH; Award Amount: $443,945

Mike Wannemuehler (Co-PI)
K. J. Yoon (Co-PI)

Exercise-induced immunomodulation in the Aged: Mechanisms
Sponsor: NIH; Award Amount: $492,875

Richard Martin (PI)
Modulation and Resistance of Levamisole Receptor Channels
Sponsor: NIH; Award Amount: $247,677

Darrell Trampel (PI)
James Roth (Co-PI)

A Federal and State Transport Plan for Movement of Turkeys from Turkey Farms in a High Pathogenicity Avian Influenza Control Area to Processing Plants: A FAST Turkeys Plan
Sponsor: USDA/APHIS; Award Amount: $181,703

Diana Peterson (PI)
Amygdalar Modulation of Processing in Auditory Cortex
Sponsor: NIH; Award Amount: $148,000

Frank Minion (PI)
Mike Wannemuehler (Co-PI)
Gregory Phillips (Co-PI)

Novel Therapies for Pneumonic Plague Targeting Quorum Sensing Components
Sponsor: Office of Naval Research; Award Amount: $980,944

Arthi Kanthasamy
Neuroinflammatory Mechanisms in the Progression of Parkinsons Disease
Sponsor: National Institutes of Health; Award Amount: $318,624

James Roth (PI)
Sheela Ramamoorthy (Co-PI)

SIV epitopes and genetic factors that affect surveillance and immunity elicited by influenza vaccines against emerging influenza.
Sponsor: USDA/ARS; Award Amount: $500,000

Derald Holtkamp (PI)
A cross-sectional study of PRRSV Positive Swine Breeding Herd Sites to evaluate associations between risk factors and a case definition-based number and severity of clinical PRRS episodes
Sponsor: Boehringer Ingelheim Vetmedica; Award Amount: $149,200

Tanja Opriessnig (PI)
Pat Halbur (Co-PI)

Studies to investigate the role of spray-dried plasma in PCV2 transmission.
Sponsor: APC, Inc.; Award Amount: $123,666

Jeff Zimmerman (PI)
Annette O'Connor (Co-PI)
Derald Holtkamp (Co-PI)

Identifying ecologic and epidermiologic factors in the control of PRRS: A field-based approach
Sponsor: Kansas State University; Award Amount: $123,241

Katherine Gibson-Corley (PI)
Role of B cells in the cell-mediated immune response to leishmania amazonensis
Sponsor: NIH; Award Amount: $120,057

Steven Ensley (Co-PI)
Increasing the inclusion rate of distillers grains in beef cattle diets through mitigation of sulfate toxicity
Sponsor: ADM Alliance Nutrition; Award Amount: $62,271

Steven Ensley (Co-PI)
Increasing the inclusion rate of distillers grains in beef cattle diets through mitigation of sulfate toxicity
Sponsor: Archer Daniels Midland; Award Amount: $56,708

Tanja Opriessnig (PI)
Pat Halbur (Co-PI)

The prevalence of PCV2 viremia in conventional piglets born to PCV2-vaccinate and non-vaccinated sows at birth and effect of PCV2 viremia on pig performance.
Sponsor: National Pork Board; Award Amount: $48,170

Rodney Baker (PI)
Study of PRRS and PRRS related diseases
Sponsor: USDA/ARS; Award Amount: $38,577

Jared Danielson (PI)
Veterinary curricular cooperative: Reducing veterinary student debt loads through electronic sharing of courses and textbooks.
Sponsor: University of Wisconsin; Award Amount: $16,933

Suzanne Millman (PI)
Raymond Brooks (Co-PI)
Locke Karriker (Co-PI)

A hands-on education and training module for euthanasia in Iowa swine welfare school
Sponsor: Iowa Pork Producers Association; Award Amount: $14,356

Suzanne Millman (PI)
Effectiveness of non-penetrating captive bolt and restraint for euthanasia of piglets from birth to 9 kg.
Sponsor: University of Guelph; Award Amount: $11,000

Suzanne Millman (Co-PI)
Midwest poultry research program.
Sponsor: USDA/NIFA; Award Amount: $438,345

Sinisa Grozdanic (Co-PI)
Stem cell-mediated delivery of neurotrophic factors for treatment of glaucoma.
Sponsor: NIH; Award Amount: $354,007

Vlastislav Bracha (PI)
Intermediate cerebellum in adaptive reflex control
Sponsor: NIH; Award Amount: $313,465

Richard Martin (Co-PI)
High-resolution electrical sensing of surface charges and currents of living cells.
Sponsor: NSF; Award Amount: $279,965

Qijing Zhang (PI)
Mechanisms of antibiotic efflux in Campylobacter
Sponsor: NIH; Award Amount: $260,565

James Roth (PI)
Development of operational guidelines for vaccination for hoof and mouth disease, vaccination for classical swine fever and vaccination for avian influenza.
Sponsor: USDA/APHIS; Award Amount: $247,500

James Roth (PI)
Renee Dewell (Co-PI)

Development of cow-calf facility manual for members of the National Animal Health Emergency Response Corps.
Sponsor: USDA/APHIS; Award Amount: $167,200

Elizabeth Riedesel (Co-PI)
Intratherecal enzyme therapy for mucopolysaccharidosis I.
Sponsor: Harbor University; Award Amount: $130,752

Darrell Trampel (PI)
James Roth (Co-PI)

Federal and state transport plan for movement of eggs and egg products from non-infected premises within an avian influenza control area (FAST Eggs Plan).
Sponsor: USDA/APHIS; Award Amount: $119,900

James Roth (PI)
Janice Mogan (Co-PI)
Kevan Flaming (Co-PI)

Enhancing and maintaining the training website for the National Animal Health Emergency Response Corps.
Sponsor: USDA/APHIS; Award Amount: $84,700

Michael Wannemuehler (PI)
Glenn Songer (Co-PI)

Clostridial dermatitis in turkeys.
Sponsor: USDA/APHIS; Award Amount: $70,000

Suzanne Millman (PI)
Evaluation of the rate of administration of various gas mixtures using the smart box euthanasia device as a humane and effective method of piglet euthanasia.
Sponsor: Value-Added Science and Technologies; Award Amount: $55,165

Lisa Nolan (PI)
Animal health and disease research
Sponsor: USDA/NIFA; Award Amount: $53,948

Terry Engelken (Co-PI)
Investigating bovine respiratory disease effects on beef quality and effectiveness of genomic tests for beef quality
Sponsor: Iowa Beef Industry Council; Award Amount: $35,922

Sinisa Grozdanic (PI)
Intergovernmental Personnel Act Assignment for Sinisa Grozdanic
Sponsor: Veterans Administration; Award Amount: $33,000

Locke Karriker (Co-PI)
Evaluation of a boron product in the melioration of osteochondrosis in pigs.
Sponsor: U.S. Borax, Inc.; Award Amount: $28,376

Rodney Baker (PI)
Development of an epitope-based vaccine against swine influenza A virus using a non-toxic enterotoxin as the carrier-adjuvant
Sponsor: South Dakota State University; Award Amount: $17,530

Steven Carlson (PI)
Investigation of Elmeria Macusaniensis-salmonella co-infections in alpacas
Sponsor: Morris Animal Foundation; Award Amount: $4,000

Suzanne Millman (PI)
Is behavior during the pre-race period and avoidance responses at the starting gate predictive of breakdown during horse races?
Sponsor: Morris Animal Foundation; Award Amount: $4,000

 

ILHAC Funds New Research Projects

The Iowa Livestock Health Advisory Council has funded four new programs:

Alan Robertson, Richard Martin (BMS)
Determining the mode of action of the new anthelminitic tribendimidine

Orhan Sahin, Qijing Zhang (VMPM)
A mouse infection model for assessment of pathogenic mechanism of a highly virulent Campylobacter jejuni close associated with sheep abortion

Charles Thoen (VMPM)
Evaluation of reduced does of Mycopar (Mycobacterium paratuberculosis-heat-killed cells in oil) for use to vaccinate calves in a Johne’s positive herd: A Field Study

Jessie Trujillo, Peter Nara (VMPM)
Development and validation of a real-time PCR detection and differentiation assay for pathogenic Mycoplasma species in various animal species

The following programs have received a continuation of funding from ILHAC:

Doug Jones, Jesse Hostetter (VPATH)
Development of an in vivo diagnostic for Johne’s disease using the merging technologies of nanoparticles and “smart” copolymers

Jenny Li (WMPM)
Characterization of three putative autotransporters of Avian Pathogenic E.coli and their potential use as vaccines against laying hen peritonitis

Peter Nara, Tanja Opriessnig, Jessie Trujillo (BMS)
Developing a broadly protective PRRS vaccine

 

HLI Funds Research Awards

The Healthy Livestock Initiative has announced $133,000 in 2010-2011 awards to CVM researchers.

Bradley Blitvich (VMPM)
Significance of a novel -1 ribosomal frame-shift protein uniquely encoded by insect-specific flaviviruses
Award Amount: $8,000

Ganwu Li (VMPM)
Novel Two-Component Signal Transduction System in EHEC Virulence
Award Amount: $10,000

Mark Ackermann, Albert Van Geelen (VPATH)
An ovine model of severe RSV disease
Award Amount: $15,000

Jesse Hostetter, Mike Wannemuehler, Claire Andreasen (VPATH)
Intestinal epithelial barrier: minimizing injury by opportunistic pathogens
Award Amount: $20,000

Greg Phillips, Al Jergens, Amanda Ramer-Tair, Mike Wannemuehler (VMPM)
Use of new mutagenesis strategy to identify genes important for Salmonella enterica serovar Typhimurium survival in the mammalian host
Award Amount: $20,000

Amanda Ramer-Tait, Chris Tuggle, Mike Wannemuehler
Effect of swine barn dust exposure on macrophage function: implications for swine respiratory health
Award Amount: $10,000

Michael Kimber, Lyric Bartholomay (BMS)
Developing an RNA Interference assay to validate novel drug targets in animal parasitic nematodes
Award Amount: $20,000

Chris Minion, Erin Strait (VMPM)
Evaluation of Mycoplasma hyopneumoniae surface protein diversity through genome sequencing and in silico antigenic analysis
Award Amount: $15,000

Pat Gorden, Paul Plummer, Greg Phillips, Chong Wang (VDPAM)
Development of a Metagenomics Tool Box and Application of Metagenomics to Address Culture Negative Clinical Mastitis Samples
Award Amount: $15,000

 


 

Summer Scholars Hard At Work

Twenty-five students and 16 mentors are participating in this year’s Summer Scholars program. Students are matched with faculty and staff mentors to expose them to veterinary medical research through participation in valuable discovery activities. The program started with orientation on May 17 and will end with a poster session and awards on Research Day, August 13. Mentors include Drs.

Holly Bender Doug Jones Greg Sommerville
Steve Carlson Cathy Miller Erin Strait
Jesse Goff Suzanne Millman Brett Sponseller
Patrick Gorden Peter Nara Jessie Trujillo
Sinisa Grozdanic Christy Petersen  
Jesse Hostetter Greg Phillips  

 

 

 

 

 

Twenty-five projects include those funded by ILHAC, NIH, Merial, Boehringer Ingelheim Vetmedica, and mentors.

Sarah Kumpula, Mark Madsen
Mentor: Holly Bender
Transforming complex problem-solving education by re-engineering the Diagnostic Pathfinder into ThinkSpace: a multidisciplinary team-based approach to educational research, software development and community building.

Matt Brewer, Michelle Rutz-Mendicino
Mentor: Steve Carlson
Differentiation of hornless Holsteins with increased risk of salmonellosis

Chad Clancy
Mentor: Jesse Goff
Effect of diet calcium and vitamin D metabolites on inflammatory bowel disease (IBD)

Blaine Nicks
Mentor: Patrick Gorden
Characterization of the epidemiology, lesion development and immune response between normal cows and cows affected with papillomatous digital dermatitis.

Jessica Noble
Mentor: Sinisa Grozdanic
Immunological characterization of cancer associated retinopathy

Nicholas Sterner
Mentor: Sinisa Grozdanic
Neuroprotective strategies for the traumatic brain injury

Lisa Trout
Mentor: Sinisa Grozdanic
Role of VEGF in systemic and ocular abnormalities in patients with autoimmune retinopathies

Stephanie Klein
Mentor: Jesse Hostetter
Evaluation of early immune responses of cattle infected with Mycobacterium avium subspecies paratuberculosis (MAP)

Jared Voge
Mentors: Doug Jones, Jesse Hostetter
Development of an in vivo diagnostic for Johne's disease using the emerging technologies of nanoparticles and "smart" copolymers

Alex Miller, Lindsay Junk
Mentor: Cathy Miller
Investigation of reovirus as a therapeutic vector for hypoxic tumor cells

Megan Bullis
Mentor: Suzanne Millman
Racehorse behavior while entering the starting gates as an indicator of breakdown

Cole Burrack
Mentor: Suzanne Millman
Investigation into the reliability of observational lameness analysis in sows

Dimitar Spasov
Mentor: Suzanne Millman
Investigation of bovine stress associated with tilt table and lift restraint devices for hoof trimming

Laura Hamelin
Mentors: Peter Nara, Jessie Trujillo
Evaluation of Mycoplasma hyopneumoniae surface protein diversity

Kristin Frank
Mentor: Christine Petersen
The role of TNF – a alleles in promoting clinical canine visveral Leishmaniasis

Mandy Larson
Mentor: Christine Petersen
Beta testing a biologic risk assessment tool at local animal shelters

Amanda Gerard
Mentor: Greg Phillips
Understanding antibiotic tolerance in human and animal pathogens

Sarah Vitosh
Mentor: Greg Sommerville
Staphylococcal metabolic sensing: implications for bovine mastitis detection and treatment

Sarah Wiechert
Mentor: Brett Sponseller
Acquisition of pulmonary immunocompetency in the horse

Kimberly Schultz
Mentor: Erin Strait
Optimization of antibiotic sensitivity testing for Mycoplasma hyosynoviae and hyorhinis

Kelsey Vinnedge
Mentors: Peter Nara, Jessie Trujillo
Development, validation and application of a real-time PCR detection and differentiation assay for pathogenic mycoplasm species in various animal species

Alejandro Larios
Mentors: Peter Nara, Jessie Trujillo
Developing a broadly protective PRRS vaccine

 


 

Vice-President of Research and Economic Development Visits with ILHAC Group

Sharron Quisenberry, ISU Vice President for Research and Economic Development, spoke at the March meeting of the Iowa Livestock Health Advisory Council (ILHAC) at the College of Veterinary Medicine. She encouraged representatives of Iowa’s livestock industry to join the University in developing a strong, more proactive collaborative effort to promote research in animal agriculture.

Quisenberry stated that while reduced budgets have caused a number of schools of veterinary medicine to reduce their support for livestock industries, Iowa State is maintaining a committed, strong and supportive role. This comes at a good time as producers are finding it difficult to stay in business due to increasing costs, fluctuations in supply and demand, and complications from issues like welfare, disease transmission, safety and the environment.

“Research at the university is transitioning to better serve the industry with applicable science that enables producers to diversify, meet changing needs , and remain viable business entities,” said Quisenberry. “The university will focus on creating systems that will keep producers in business, get people into rural areas, and support them with needed veterinarians and veterinary services.”

Quisenberry cited a changing national research agenda that is focusing more on basic than applied research. The National Academy is focusing on four challenges, one being food and related issues. The USDA is focusing on five areas of basic research that include food safety and production. The University is committed to developing and strengthening collaborative efforts with ILHAC and others that will transition this basic research to applications that will improve the industry.

A key to successfully making this transition is activity in the political arena. “We need to establish networks that get clear and concise messages to policy makers and legislators in Iowa and Washington, D.C. regarding our capabilities and needs,” said Quisenberry. “This will help set national agendas regarding production agriculture, keep livestock issues and positions in front of policy makers, and help sell a collaborative research agenda that takes laboratory research to the field, like that offered by ILHAC and ISU. ILHAC and the livestock industry can get in front of the right people and help set the national agenda. Likewise, by collectively planning to advantageously manage the state budget line item, we can leverage the dollars to produce research and applications that best serve the state and its production agriculture industry.”

“Iowa State has a unique research system with its colleges of agriculture and veterinary medicine, centers, institutes and partnerships,” said Quisenberry, “but we need to use our partnerships to do a better job of marketing products and science.” Toward this end, ISU is also working to create synergies between Iowa’s three state universities and with outside groups like the I-70 corridor.

Strong teams are being developed that will move bench research to field use in the state. For example, collaborations between ISU veterinary medicine and engineering colleges are developing vaccines and new methods of delivering them, and taking MRI and CT images and making them 3-D to improve healthcare and treatment. Five USDA labs and one Department of Energy lab in Ames provide additional resources.

The convergence of animal and human health into “one health” will be the focus of a symposium to be held at ISU this fall. The gathering will discuss how faculty efforts can make an impact, not only at Iowa State, but across other institutions as well. Drs. James Bloedel and Lisa Nolan are heading the ISU effort.

“Our goal is to be the best in the nation, but we need to work with the livestock industry to assemble the best people and create the best systems in the world that will enable us to do our best for agriculture. At ISU we intend to think outside the box . . . in fact break the box . . . as we create a systems approach to animal and human health,” said Quisenberry.

“The future requires us to produce more food than ever. Four things have consistently differentiated the U.S. from other countries: A stable government; strong educational system; strong work ethic; and a constant, quality food supply. A strong working relationship between Iowa State and ILHAC will help ensure that our research programs support and improve this position.”

 


 

Deadlines You Should Know About

UPCOMING GRANT DEADLINES
NIH Deadlines: http://grants.nih.gov/grants/funding/submissionschedule.htm
USDA AFRI Deadlines: www.grants.gov
NSF Deadlines: http://www.nsf.gov/funding/pgm_list.jsp?org=NSF&ord=date