Feline

Pathogen: 
Influenza A virus - IAV^
Species: 
Canine, Equine, Feline, Porcine
Section: 
Molecular
Test: 
Sequencing (hemagglutinin (HA), ~1.7kb)
Standard Fee: 
$110.00
Best Practice Fee: 
$99.00
Days Tested: 
M, W, F
Turn-Around: 
up to two business weeks from time of request
Specimen: 
BAL, lungs, nasal swabs, oral fluid, bronchial swabs, udder wipe, and virus isolates
Additional Comments: 
USDA surveillance samples may be delayed while the required isolate is obtained. Sample amount: no less than 1mL fluid, 3 grams of tissue Success rate: IAV screening PCR with a ct of 35 or lower for lung, BAL and nasal swab and a ct of 30 or lower for oral fluids is recommended. Cost: IAV HA gene PCR-$35.00 IAV Sequencing and analysis- $75.00 Total cost=$110.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
File attachment: 
Pathogen: 
Influenza A virus - IAV
Species: 
Canine, Equine, Feline, Porcine
Section: 
Molecular
Test: 
PCR - Screen only
Standard Fee: 
$25.00
Best Practice Fee: 
$22.50
Days Tested: 
M-F
Turn-Around: 
same day
Specimen: 
lung, nasal swab, BAL, oral fluids, bronchial swab, allantoic fluid, feces, oropharyngeal swab, pharyngeal swab, tracheal swab, udder wipe
Additional Comments: 
Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available. Follow up IAV subtyping added on after initial testing will incur an additional fee.
Pathogen: 
Influenza A virus - IAV
Species: 
Canine, Equine, Feline, Porcine
Section: 
Molecular
Test: 
PCR - Screen and Subtype (positives)
Standard Fee: 
$35.00
Best Practice Fee: 
$31.50
Days Tested: 
M-F
Turn-Around: 
screen - same day; subtype - next day
Specimen: 
lung, nasal swab, BAL, oral fluids, bronchial swab, allantoic fluid, feces, oropharyngeal swab, pharyngeal swab, tracheal swab, udder wipe
Additional Comments: 
Includes subtyping all positive samples. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available.

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