Sample amount: no less than 1mL fluid or 3 grams of tissue.
Success rate: Rotavirus PCR with a ct of 30 or lower is recommended regardless of serotype.
Cost: Rotavirus clone VP7 PCR- $70.00, Rotavirus VP7 sequencing and analysis- $60.00: Total cost= $130.00
Includes subtyping of all positive samples per case. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory.
Star Swab II, Copan swabs, Other swabs are also available.