Brian Michael Lee

PhD
Research Assistant Professor
515-294-8494
2046 Vet Med
Education & Certifications  
  • Postdoctoral training, 2003, The Scripps Research Institute, La Jolla, California
  • PhD, Biochemistry, 1997, University of Maryland Graduate School, Baltimore
  • BS, Chemistry, 1989, College of William and Mary, Williamsburg, Virginia
Teaching  

  • 2015-2016                                      Instructor-in-charge, BMS 575, Cell Biology

  • 2014                                                Instructor-in-charge, CHEM 350, Biological Chemistry

  • 2013                                                Instructor-in-charge, CHEM 452, Advanced Biological Chemistry

  • 2013                                                Instructor-in-charge, CHEM 350, Biological Chemistry

  • 2012                                                Instructor-in-charge, CHEM 352, Advanced Biological Chemistry

  • 2012                                                Instructor-in-charge, CHEM 350, Biological Chemistry

  • 2011                                                Instructor-in-charge, CHEM 559, Advanced Topics in Biological Chemistry: Protein NMR Spectroscopy

  • 2011                                                Instructor-in-charge, CHEM 350 Biological Chemistry

  • 2010                                                Instructor-in-charge, CHEM 352, Advanced Biochemistry

  • 2010                                                Instructor-in-charge, CHEM 350, Introduction to Biological Chemistry

  • 2009                                                Instructor-in-charge, CHEM 559, Advanced Topics in Biological Chemistry

  • 2009                                                Instructor-in-charge, CHEM 210, General and Inorganic Chemistry

  • 2008-2009                                      Instructor-in-charge, CHEM 350, Introduction to Biological Chemistry

  • 2007                                                Instructor-in-charge, CHEM 569, Advanced Topics in Physical Chemistry: Protein Chemistry

  • 2007                                                Instructor-in-charge, CHEM 350, Introduction to Biological Chemistry

  • 2007                                                Instructor-in-charge, CHEM 351, Biochemistry Laboratory

  • 2007                                                Instructor-in-charge, CHEM 569, Advanced Topics in Physical Chemistry

  • 2006                                                Instructor-in-charge, CHEM 350, Introduction to Biological Chemistry
Research Focus & Interests  

Translational regulation of virulence and cellular development, structural biology, protein-RNA interactions, molecular recognition, protein dynamics, zinc-finger proteins, NMR spectroscopy.

Honors & Awards  
  • Ruth L. Kirschstein National Research Service Award for Individual Postdoctoral Training, National Institutes of Health, 5S rRNA Recognition by Transcription Factor IIIA, (1999 - 2001)

Leadership/Committees/Council  
  • Macromolecular X-ray Crystallography User Group, Iowa State University (2015 - Present)
  • NMR Facility Committee and Spectrometer Oversight, SIUC (2008 - 2014)
  • Vaccines Against Antigenically Variable Viruses (VAAVV), Session Chair (November 5-8, 2015)
  • Great Plains Regional Annual Symposium on Protein & Biomolecular NMR (GRASP-NMR), Organizing Committee and Session Chair (November 2-3, 2012)
  • Symposium on Frontiers in Biomolecular NMR, Vanderbilt University, Session Co-Chair, Nashville, Tennessee (May 4-5, 2012)
  • Gibbs Conference on Biothermodynamics, Local Apples Procurement (2009 - 2013)
Memberships  
  1. The Biophysical Society (2009 - Present)
  2. The American Society for Biochemistry and Molecular Biology (2013 - Present)
Selected Publications  
  1. A. Martin-Gallardo, W.R. McCombie, J.D. Gocayne, M.G. Fitzgerald, S. Wallace, B.M. Lee, J. Lamerdin, S. Trapp, J.M. Kelley, L.I. Liu, M. Dubnick, L.A. Johnstondow, A.R. Kerlavage, P. Dejong, A. Carrano, C. Fields and J.C. Venter (1992) "Automated DNA sequencing and analysis of 106 kilobases from human chromosome 19q13.3." Nature Genetics 1 (1) 34-39. doi:10.1038/ng0492-34
  2. Paul R. Blake, Brian M. Lee, Michael F. Summers, Michael W. W. Adams, Jac-Bum Park, Zhi Hao Zhou and Ad Bax (1992) "Quantitative measurement of small through-hydrogen-bond and ’through-space’ 1H-113Cd and 1H-199Hg J couplings in metal-substituted rubredoxin from Pyrococcus furiosus." Journal of Biomolecular NMR 2 (5) 527-533. doi:10.1007/BF02192814

  3. Paul R. Blake, Brian Lee, Michael F. Summers, Jae-Bum Park, Zhi Hao Zhou and Michael W. W. Adams (1994) "Heteronuclear magnetic resonance studies of Zn, 113Cd, and 199Hg substituted P. furiosus rubredoxin: implications biological electron transfer." New Journal of Chemistry 18 (3) 387-395.

  4. Cory Momany, Ladislau C. Kovari, Andrew J. Prongay, Walter Keller, Rossitza K. Gitti, Brian M. Lee, Alexander E. Gorbalenya, Liang Tong, Jan McClure, Lorna S. Ehrlich, Michael F. Summers, Carol Carter and Michael G. Rossman (1996) "Crystal structure of dimeric HIV1 capsid protein." Nature Structural Biology 3 (9) 763-770. doi:10.1038/nsb0996-763

  5. Rossitza K. Gitti†, Brian M. Lee†, Jill Walker, Michael F. Summers, Sanghee Yoo and Wesley I. Sundquist (1996) "Structure of the amino-terminal core domain of the HIV-1 capsid protein." Science 273 (5272) 231-235. doi:10.1126/science.273.5272.231

  6. Brian M. Lee, Roberto N. de Guzman, Brian G. Turner, Nico Tjandra and Michael F. Summers (1998) “Dynamical behavior of the HIV-1 nucleocapsid protein.” Journal of Molecular Biology 279 (3) 633-649. doi:10.1006/jmbi.1998.1766

  7. Laura S. Neely, Brian M. Lee, Jing Xu, Peter E. Wright and Joel M. Gottesfeld (1999) "Identification of a minimal domain of 5S ribosomal RNA sufficient for high affinity interactions with the RNA-specific zinc fingers of transcription factor IIIA." Journal of Molecular Biology 291 (3) 549-60. doi:10.1006/jmbi.1999.2985

  8. John H. Laity, Brian M. Lee and Peter E. Wright. (2001) “Zinc finger proteins: new insights into structural and functional diversity.” Current Opinion in Structural Biology 11 (1) 39-46. doi:10.1016/S0959- 440X(00)00167-6

  9. Glen B. Legge, Maria A. Martinez-Yamout, David M. Hambly, Tam Trinh, Brian M. Lee, H. Jane Dyson and Peter E. Wright (2004) “ZZ domain of CBP: an unusual zinc finger fold in a protein interaction module.” Journal of Molecular Biology 343 (4) 1081-1093. doi:10.1016/j.jmb.2004.08.087

  10. Brian M. Lee, Jing Xu, Bryan Clarkson, Maria A. Martinez-Yamout, H. Jane Dyson, David A. Case, Joel M. Gottesfeld and Peter E. Wright (2006) “Induced fit and “lock-and-key” recognition of 5S RNA by zinc fingers of transcription factor IIIA.” Journal of Molecular Biology 357 (1) 275-91. doi:10.1016/j.jmb.2005.12.010

  11. Brian M. Lee†, Bethany A. Buck-Koehntop†, Maria A. Martinez-Yamout, H. Jane Dyson and Peter E. Wright (2007) “Embryonic neural inducing factor Churchill is not a DNA-binding zinc finger protein: solution structure reveals a solvent-exposed beta-sheet and zinc binuclear cluster.” Journal of Molecular Biology 371 (5) 1274-89. doi:10.1016/j.jmb.2007.06.021

  12. Raphael Stoll, Brian M. Lee, Erik W. Debler, John H. Laity, Ian A. Wilson, H. Jane Dyson and Peter E. Wright (2007) “Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA.” Journal of Molecular Biology 372 (5) 1227-45. doi:10.1016/j.jmb.2007.07.017.

  13. Brian Lee (2012) “Nab2, a three-cornered hat to bind a poly(A) tail.” Structure 20 (6) 937-938. doi:10.1016/j.str.2012.05.005

  14. Daniel J. Merkel*, Sarah B. Wells*, Bryce C. Hilburn*, Fatima Elazzouzi, Gabriela C. Pérez-Alvarado and Brian M. Lee (2013) “The C-terminal region of cytoplasmic polyadenylation element binding protein is a ZZ domain with potential for both nucleic acid and protein-protein interactions.” Journal of Molecular Biology 425 (11) 2015-2026. doi:10.1016/j.jmb.2013.03.009

  15. Natalia N. Singh, Brian M. Lee and Ravindra N. Singh (2015) “Splicing regulation in spinal muscular atrophy by a unique RNA structure formed by long distance interactions,” Annals ofthe New York Academy of Sciences 1341: 176-187. doi:10.1111/nyas.12727

  16. Natalia N. Singh, Brian M. Lee, Christine J. DiDonato and Ravindra N. Singh (2015) “Mechanistic principles of antisense targets for the treatment of spinal muscular atrophy,” Future Medicinal Chemistry 7 (13) 1793-1808. doi:10.4155/fmc.15.101

  17. Joonbae Seo, Natalia N. Singh, Eric Ottesen, Brian M. Lee and Ravindra Singh (2016) “A novel human-specific splice isoform alters the critical C-terminus of Survival Motor Neuron protein,” Scientific Reports, 6: 30778. doi: 10.1038/srep30778

Link to PubMed