Brian Michael Lee

Research Assistant Professor
2046 Vet Med
Education & Certifications  

Postdoctoral training, The Scripps Research Institute, La Jolla, California, 2003.

Ph.D., Biochemistry, University of Maryland Graduate School, Baltimore, 1997.

B.S., Chemistry, College of William and Mary, Williamsburg, Virginia, 1989.

Research Focus & Interests  

Translational regulation of virulence and cellular development, structural biology, protein-RNA interactions, molecular recognition, protein dynamics, zinc-finger proteins, NMR spectroscopy.

Selected Publications  

Daniel J. Merkel*, Sarah B. Wells*, Bryce C. Hilburn*, Fatima Elazzouzi, Gabriela C. Pérez-Alvarado and Brian M. Lee (2013) “The C-terminal region of cytoplasmic polyadenylation element binding protein is a ZZ domain with potential for both nucleic acid and protein-protein interactions.” Journal of Molecular Biology 425 (11) 2015-2026. doi:10.1016/j.jmb.2013.03.009

Brian Lee (2012) “Nab2, a three-cornered hat to bind a poly(A) tail.” Structure 20 (6) 937-938. doi:10.1016/j.str.2012.05.005

Raphael Stoll, Brian M. Lee, Erik W. Debler, John H. Laity, Ian A. Wilson, H. Jane Dyson and Peter E. Wright (2007) “Structure of the Wilms Tumor Suppressor Protein Zinc Finger Domain Bound to DNA.” Journal of Molecular Biology 372 (5) 1227-45. doi:10.1016/j.jmb.2007.07.017

Brian M. Lee†, Bethany A. Buck-Koehntop†, Maria A. Martinez-Yamout, H. Jane Dyson and Peter E. Wright (2007) “Embryonic neural inducing factor Churchill is not a DNA-binding zinc finger protein: solution structure reveals a solvent-exposed beta-sheet and zinc binuclear cluster.” Journal of Molecular Biology 371 (5) 1274-89. doi:10.1016/j.jmb.2007.06.021

Brian M. Lee, Jing Xu, Bryan Clarkson, Maria A. Martinez-Yamout, H. Jane Dyson, David A. Case, Joel M. Gottesfeld and Peter E. Wright (2006) “Induced fit and “lock-and-key” recognition of 5S RNA by zinc fingers of transcription factor IIIA.” Journal of Molecular Biology 357 (1) 275-91. doi:10.1016/j.jmb.2005.12.010 

Glen B. Legge, Maria A. Martinez-Yamout, David M. Hambly, Tam Trinh, Brian M. Lee, H. Jane Dyson and Peter E. Wright (2004) “ZZ domain of CBP: an unusual zinc finger fold in a protein interaction module.” Journal of Molecular Biology 343 (4) 1081-1093. doi:10.1016/j.jmb.2004.08.087

John H. Laity, Brian M. Lee and Peter E. Wright. (2001) “Zinc finger proteins: new insights into structural and functional diversity.” Current Opinion in Structural Biology 11 (1) 39-46. doi:10.1016/S0959-440X(00)00167-6

Laura S. Neely, Brian M. Lee, Jing Xu, Peter E. Wright and Joel M. Gottesfeld (1999) "Identification of a minimal domain of 5S ribosomal RNA sufficient for high affinity interactions with the RNA-specific zinc fingers of transcription factor IIIA." Journal of Molecular Biology 291 (3) 549-60. doi:10.1006/jmbi.1999.2985

Brian M. Lee, Roberto N. de Guzman, Brian G. Turner, Nico Tjandra and Michael F. Summers (1998) “Dynamical behavior of the HIV-1 nucleocapsid protein.” Journal of Molecular Biology 279 (3) 633-649. doi:10.1006/jmbi.1998.1766

Rossitza K. Gitti†, Brian M. Lee†, Jill Walker, Michael F. Summers, Sanghee Yoo and Wesley I. Sundquist (1996) "Structure of the amino-terminal core domain of the HIV-1 capsid protein." Science 273 (5272) 231-235. doi:10.1126/science.273.5272.231

Cory Momany, Ladislau C. Kovari, Andrew J. Prongay, Walter Keller, Rossitza K. Gitti, Brian M. Lee, Alexander E. Gorbalenya, Liang Tong, Jan McClure, Lorna S. Ehrlich, Michael F. Summers, Carol Carter and Michael G. Rossman (1996) "Crystal structure of dimeric HIV1 capsid protein." Nature Structural Biology 3 (9) 763-770. doi:10.1038/nsb0996-763 

Paul R. Blake, Brian Lee, Michael F. Summers, Jae-Bum Park, Zhi Hao Zhou and Michael W. W. Adams (1994) "Heteronuclear magnetic resonance studies of Zn, 113Cd, and 199Hg substituted P. furiosus rubredoxin: implications biological electron transfer." New Journal of Chemistry 18 (3) 387-395.

Paul R. Blake, Brian M. Lee, Michael F. Summers, Michael W. W. Adams, Jac-Bum Park, Zhi Hao Zhou and Ad Bax (1992) "Quantitative measurement of small through-hydrogen-bond and ’through-space’ 1H-113Cd and 1H-199Hg J couplings in metal-substituted rubredoxin from Pyrococcus furiosus." Journal of Biomolecular NMR 2 (5) 527-533. doi:10.1007/BF02192814

A. Martin-Gallardo, W. R. McCombie, J. D. Gocayne, M. G. Fitzgerald, S. Wallace, B. M. Lee, J. Lamerdin, S. Trapp, J. M. Kelley, L. I. Liu, M. Dubnick, L. A. Johnstondow, A. R. Kerlavage, P. Dejong, A. Carrano, C. Fields and J. C. Venter (1992) "Automated DNA sequencing and analysis of 106 kilobases from human chromosome-19q13.3." Nature Genetics 1 (1) 34-39. doi:10.1038/ng0492-34

These authors contributed equally.

* Students mentored by BML.

Link to PubMed