Diagnostic Tests
A $10 submission fee and 1% hazardous fee is assessed on each case.
Testing Schedule
Our goal is to provide accurate results and timely service. However, many research and diagnostic assays are not performed daily and/or testing schedules may change. If your case involves testing animals for export, show animals, animals to be sold, and/or a large number of samples, please contact the VDL (515-294-1950) prior to submission to verify the availability and turnaround time.
Fees and test schedules are subject to change without notice.
For additional test information, please click the pathogen name.
^ Testing performed in part or in total at a Referral Laboratory.
^^ Assay has not been fully validated for all the testing conducted.
| Pathogen | Species | Test | Standard Fee | Best Practice Fee |
Days Tested |
Turn-Around | Specimen | File attachment | Comments |
|---|---|---|---|---|---|---|---|---|---|
| Ergovaline | All Species | quantitation | $90.00 | 5-7 days | feed/forage (20g) | ||||
| Toxic Elements | All Species | $35.00 | 3-5 days | liver |
Panel includes: Antimony, arsenic, barium, boron, cadmium, calcium, chromium, cobalt, copper, iron, lead, magnesium, manganese, mercury, molybdenum, phosphorus, potassium, selenium, sodium, thallium, and zinc
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| All Species | RNAscope® |
Please call for pricing and availability.
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| Ethylene glycol | All Species | $50.00 | 5-7 days | serum (2mL), stomach / rumen contents (50g), kidney (50g), bait (50g), urine (5mL), plasma, water, liver, feed | |||||
| Trace mineral panel - serum/water | All Species | ICPMS | $50.00 | 3-5 days | serum (2mL), water (100mL), plasma (2mL) |
Individual minerals $25 each. Panel includes 10 minerals: Calcium, Copper, Iron, Potassium, Magnesium, Manganese, Molybdenum, Phosphorus, Selenium, Zinc. ICPMS video
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| Mycoplasma hyorhinis - MHR | Porcine | RNAscope® |
Please call for pricing and availability.
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| Feed particle size | All Species | $20.00 | 3-5 days | feed (200g) |
Not for high-fat feeds. Feed Guidelines link
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| Trace mineral panel - feed | All Species | ICPMS | $55.00 | 3-5 days | feed (200g) |
Individual minerals $25 each. Panel includes 14 minerals: Calcium, Cadmium, Cobalt, Chromium, Copper, Iron, Potassium, Magnesium, Manganese, Molybdenum, Sodium, Phosphorus, Selenium, Zinc. ICPMS video
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| Fluoride | All Species | $100.00 | 7-10 days | serum (2mL), urine (2mL), bone - whole bone, tissue (50g) plasma, water |
Representative of whole skeleton.
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| Trace mineral panel - tissue | All Species | ICPMS | $55.00 | 3-5 days | tissue (2g) (heart, kidney, liver, liver biopsy) |
Individual minerals $25 each. Panel includes 14 minerals: Calcium, Cadmium, Cobalt, Chromium, Copper, Iron, Potassium, Magnesium, Manganese, Molybdenum, Sodium, Phosphorus, Selenium, Zinc. ICPMS video
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| GC/MS Toxicant Screen | All Species | $150.00 | 7-10 days | feed (200g), stomach contents (50g), water (100mL), serum (2mL), tissues (50g), other |
Chromatography/Mass Spectroscopy (GC/MS) screen offers the possibility of using libraries of mass spectra of more than 200,000 compounds. The GC/MS screen is capable of detecting organochlorines, organophospates, carbamates, aliphatic hydrocarbons, industrial pollutants, disinfectants, antioxidants, miscellaneous pesticides, polycyclic aromatics, alkyl benzenes, some natural products, vitamins and some drugs. If the matrix of interest is not listed, please contact the lab for further information.
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| Urea | All Species | $40.00 | 5-7 days | feed (100g), water |
All specimens should be frozen. Feed Guidelines link
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| Gossypol | All Species | $80.00 | 7-10 days | feed (200g), stomach/rumen contents (50g) |
Feed Guidelines link
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| Vitamins A or E | All Species | $100.00 | 3-5 days | serum (2mL), liver (100g), feed (200g), plasma |
Price: $25 for serum, $32 for liver, $100 for feed.
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| Heavy Metals Panel Fluid | All Species | $50.00 | 3-5 days | whole blood - EDTA (2mL), milk, water |
Panel includes: Arsenic, Cadmium, Lead, and Mercury
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| Vitamin D^ | All Species | $275.00 | 7-10 days | feed (200g), liver (50g) |
testing referred to accredited laboratory
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| Vitamin D, 25 hydroxy^ | All Species | $42.50 | 5-7 days | serum or plasma (0.5mL) |
testing referred to accredited laboratory
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| Water Quality Panel | All Species | $40.00 | 3-5 days | water (500mL) |
For livestock suitability. Panel includes: nitrate/nitrite, total dissolved solids, sulfates, and coliform bacteria
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| Porcine Epidemic Diarrhea Virus - PEDV | Porcine | ISH | Small intestine |
Contact lab for pricing and availability.
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| Iron | All Species | $25.00 | 3-5 days | serum (5mL), feed (200g), kidney or liver (50g), whole blood, heart, milk, ocular fluid/vitreous humor, plasma, water, EDTA |
Price: $20 for serum, $25 for feed/tissue. Included in trace mineral panel. Feed Guidelines link
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| Zinc | All Species | as needed | $25.00 | 3-5 days | serum (2mL), feed (200g), liver or kidney (100g) EDTA, heart, milk, ocular fluid/vitreous humor, plasma, water |
Price: $20 for serum or urine, $25 for feed or tissue. For best results, use special trace element collection tubes (royal blue stopper). Others may be contaminated. Included in trace minerals panel. Feed Guidelines link
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| Virus isolation shipping | VI shipping | $50.00 | |||||||
| Lead | All Species | $25.00 | same day | EDTA (2mL), feed (200g), kidney or liver (50g), milk, water, heart, bait, paint |
Price: $20 for blood, $25 for feed/tissue. Included in heavy metals panel. Feed Guidelines link
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| Magnesium | All Species | $25.00 | 3-5 days | serum (2mL), feed (200g), water (100mL), EDTA, heart, kidney, liver, milk, plasma,vitreous humor/ocular fluid |
Price: $20 for serum, $25 for feed. Included in trace mineral panel. Feed Guidelines link
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| Manganese | All Species | $25.00 | 3-5 days | serum (2mL), feed (200g), water (100mL), EDTA, heart, kidney, liver, milk, ocular fluid/vitreous humor, plasma |
Price: $20 for serum, $25 for feed. Included in trace mineral panel. Feed Guidelines link
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| Mercury | All Species | $35.00 | 3-5 days | feed (200g), tissue (10g) (heart, kidney, liver), milk, serum, plasma, water |
Included in heavy metals panel. Feed Guidelines link
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| Methemoglobin | All Species | $35.00 | 3-5 days | Whole blood | |||||
| MGA (Melengestrol acetate) | All Species | feed |
See Hormone-free panel
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| Ammonia (Ammonia-Nitrogen) | All Species | $30.00 | 5-7 days | serum/plasma (2mL), rumen contents (100g), urine (2mL), aqueous vitrous humor/ocular fluid (1mL) |
Frozen (1-2 drops of saturated HgCl2 may be used instead of freezing rumen contents)
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| Pharmacological Testing |
The PhAST laboratory has the ability to test for many pharmacological analytes. Please contact the lab for availability and pricing.
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| Moisture | All Species | $10.00 | 3-5 days | feed (20g), tissue (20g), other | |||||
| Anticoagulant Panel | All Species | $95.00 | 5-7 days | EDTA (4mL), feed (200g), liver (10g) |
Panel Includes: Brodifacoum, Bromodiolone, Chlorophacinone, Coumachlor, Dicoumarol, Difenacoum, Difethialone, Diphacinone, and Warfarin
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| Arsenic | All Species | $30.00 | 5-7 days | EDTA (5mL), liver (10g), feed (200g), kidney (10g), milk, water, plasma, serum, heart |
Price: $25 for fluid samples, $30 for feed/tissue. Included in the Heavy Metals panel
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| Molybdenum | All Species | $25.00 | 3-5 days | feed (200g), EDTA, heart, kidney, liver, milk, ocular fluid/vitreous humor, serum, plasma, water |
Included in Trace minerals panel. Feed Guidelines link
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| Bone Profile | All Species | $75.00 | 3-5 days | whole 2nd rib preferred |
Price: $75 each 1-4 samples, $50 each 5 or more; Includes: ash, density, calcium, phosphorus
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| Cadmium | All Species | $25.00 | 3-5 days | tissue (heart, kidney, liver) (20g), feed (200g), EDTA, milk, vitreous humor/ocular fluid, serum, plasma, water |
Price: $20 for fluids, $25 for feed/tissue. Included in the Heavy Metals panel and the Trace Mineral panel
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| Mycotoxin - Aflatoxin | All Species | $150.00 | 7-10 days | liver (20g), milk (100mL) |
Price: $100 for liver, $150 for milk. Links to aflatoxin and mycotoxin information
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| Calcium | All Species | $25.00 | 3-5 days | serum (2mL), feed (200g), tissues (kidney, heart, liver) (20g), bone - whole bone, EDTA, plasma, milk, water, ocular fluid/vitreous humor |
Price: $20 for serum, $25 for feed, tissue and bone samples. Included in the Trace Mineral panel. Serum must not be hemolyzed - separate from clot before transit. Feed Guidelines link
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| Canine CNS Panel | All Species | $75.00 | 5-7 days | stomach / rumen contents (50g), bait (50g) |
Panel includes: 4-aminopyridine (Avitrol), Nicotine, Caffeine, Strychnine
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| Mycotoxin Tremorgens panel | All Species | $85.00 | 5-7 days | feed (200g) |
Panel Includes: Aflatrem, Penitrem A, Ergotamine, Cyclopiazonic acid, Roquefortine. Feed Guidelines link
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| Carbamate pesticide panel | All Species | $75.00 | 5-7 days | feed (200g), stomach/rumen contents (50g), water, EDTA |
Panel includes: baygon, Bux, Carbaryl, Furadan, Landrin 2,3,5 and Landrin 3,4,5, Lannate, Sulfone, Temik, Zectran. Feed Guidelines link
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| Nitrate/Nitrite | All Species | $40.00 | 3-5 days | water (50mL), ocular fluid / vitreous humor (2mL), forage/silage (100g), stomach contents (100g), serum, plasma |
Price: $30 for fluids, $40 for feed/tissue, keep refrigerated. Links to nitrate toxicity and nitrate testing video
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| Carbon monoxide (carboxyhemoglobin) | All Species | $40.00 | 5-7 days | whole blood - EDTA (2-5mL), fetal thoracic fluid (2-5mL) | |||||
| Organophosphate panel | All Species | $75.00 | 3-5 days | feed (100g), stomach/rumen contents (50g), whole blood (5mL), water |
Stomach and rumen should be composite samples. Panel includes: carbophenothion, chlorpyrifos, ciodrin, coumaphos, counter, DDVP, diazinon, dimethoate, di-syston, dyfonate, EPN, Ethyl Parathion, Famphur, Fenthion, Isofenphos, Malathion, methyl Parathion, Mevinphos, Mocap, Ronnel, Ruelene, and Thimet. See also cholinesterase. Feed Guidelines link
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| Astrovirus | Porcine | RNAscope™ ISH | Brain, spinal cord |
Please call for pricing and availability.
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| Chlorinated hydrocarbon panel | All Species | $120.00 | 5-7 days | brain (cerebrum) - half of brain, stomach/rumen contents (50g), liver (50g), body fat (10g), kidney (50g), blood EDTA (5mL), feed (200g), water, bait |
Panel includes: Aldrin, alpha-BHC, Chlordane, Dieldrin, Endosulfan I, Endosulfan II, Endrin, Hepachlor Epoxide, Heptachlor, Hexachlorobenzene, Lindane, Methoxychlor, Mirex, op DDD, op DDE, op DDT, ppp DDD, pp DDE, pp DDT, Toxaphene. Feed Guidelines link
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| PCB - polychlorinated biphenyls | All Species | $120.00 | 7-10 days | feed (200g), fat (20g) EDTA, water, stomach / rumen content |
PCBs - polychorinated biphenyls, focusing on the common representative congener PCB-1260 (1,2,3-Trichloro-4-(2,3,4-trichlorophenyl) benzene)
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| PCV3 | Porcine | RNAscope™ ISH | Call |
Please call for pricing and availability.
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| Cholinesterase (AChE) | All Species | $30.00 | same day | whole blood - EDTA (2mL), brain (refrigerated or frozen; half of cerebrum) |
Price: $25 for whole blood, $30 for brain.
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| pH | All Species | $7.00 | 2-4 days | stomach/rumen contents (50g) | |||||
| Mycoplasma hyopneumoniae - MHP | Porcine | ISH | Lung |
Please contact for pricing and availability.
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| Copper | All Species | $25.00 | 3-5 days | serum (2mL), feed (200g), liver, heart and kidney (20g), water (100mL), urine (5mL), EDTA, plasma |
Price: $20 for serum, $25 for feed, tissue and and blood. Included in the Trace Mineral panel and the Heavy Metals panel. Feed Guidelines link
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| Selenium | All Species | $25.00 | 3-5 days | serum (2mL), EDTA (10mL), feed (200g), liver (20g), hair (chronic, 50g) plasma, ocular fluid/vitreous humor, water, milk, kidney, heart |
Price: $20 for serum/whole blood, $25 for feed/tissue. Included in Trace minerals panel. Feed Guidelines link
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| Coumestrol | All Species | $85.00 | 5-7 days | feedstuffs (including cottonsead; 100g), stomach/rumen contents (50g) | |||||
| Slaframine | All Species | $100.00 | 7-10 days | hay/forage (200g) | |||||
| Custom Chemistry | All Species | $100.00 |
Price is per hour and does not include materials. The Toxicology/Nutrition lab has the analytical expertise and equipment to perform analysis on many other compunds not listed here. Please contact the lab for availability.
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| Sodium | All Species | $25.00 | 3-5 days | serum (2mL), feed (200g) plasma, EDTA, heart, kidney, liver, milk, water, ocular fluid / vitreous humor |
Price: $20 for fluids, $25 for feed/tissue. Included in the Trace Mineral panel. Feed Guidelines link
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| Brachyspira hampsonii - B hamp | Porcine | ISH | Cecum, colon |
Please call for pricing and availability.
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| Cyanide | All Species | $40.00 | 5-7 days | whole blood (4mL), liver (50g), forage/silage/feed (50g), ocular fluid/vitreous humor, plasma, serum, water, rumen/stomach content |
Freeze specimens promptly in airtight container. Feed Guidelines link
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| Sulfate | All Species | $30.00 | 3-5 days | water (100mL), feed (100g), ocular fluid/vitreous humor, plasma, serum |
Price: $20 for water, $30 for feed. Feed Guidelines link
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| Brachyspira hyodysenteriae - B hyo | Porcine | ISH | Cecum, colon |
Please call for pricing and availability.
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| Ergopeptine alkaloid panel | All Species | $90.00 | 5-7 days | feed/forage (20g) |
Panel includes: Ergovaline, Ergosine, Ergotamine, Ergocornine, Ergocryptine, and Ergocristine
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| Total dissolved solids (TDS) | All Species | $8.00 | 3-5 days | water (100mL) |
Included in Water Quality Panel
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| Individual Antibiotic Quantitation - Milk | Bovine, Caprine, Ovine, Porcine | quantitation | $150.00 | arranged | 7-10 business days | any milk products |
STAT fee of $300 for first sample, $150 for each additional sample.
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| Individual Analytes | All Species | LC-MS | $500.00 | arranged | 3 months | plasma, serum, tissue, urine |
Please call laboratory before submitting.
$500 for 1st sample, $50 for each additional sample.
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| Rotavirus group C | Bovine, Equine, Porcine | IHC | $20.00 | as needed | Small intestine | ||||
| Infectious bovine rhinotracheitis virus - IBR | Bovine | VI | $25.00 | as needed | 7-14 days | lung, nasal swab, aborted fetal tissue | |||
| Necropsy | Bovine, Camelid, Canine, Caprine, Cervidae, Equine, Feline, Ovine, Rodent, Ungulates | Necropsy - Fetal | $10.00 | as needed | Fetus/Embryo | ||||
| Toxoplasma gondii | All Species | IHC | $20.00 | as needed | brain, muscle, placenta | ||||
| Porcine epidemic diarrhea virus - PEDV^ | Porcine | Whole Genome Sequencing | $300.00 | as needed | feces, fecal swabs, intestine, oral fluids | ||||
| Necropsy | All Species | Special or complex procedures | $100.00 | as needed |
Fee is per hour
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| Transmissible gastroenteritis virus - TGEV | Porcine | IHC | $20.00 | as needed | intestine | ||||
| Porcine deltacoronavirus - PDCoV^ | Porcine | Whole Genome Sequencing | $300.00 | as needed | feces, fecal swabs, intestine, oral fluids | ||||
| Necropsy | Avian, Bovine, Camelid, Canine, Caprine, Cervidae, Feline, Ovine, Porcine, Rodent, Ungulates | Disposal ($6 per 100 lb) | $6.00 | as needed | |||||
| Bovine coronavirus - BCoV | Bovine, Caprine, Ovine | IHC | $20.00 | as needed | tissue | ||||
| Encephalomyocarditis virus - EMC | Porcine | VI | $25.00 | as needed | 7-14 days | brain, heart | |||
| Porcine sapovirus genogroup III - PSaV^^ | Porcine | Sequencing (VP1) (R&D) | $150.00 | $135.00 | As needed | 3-5 days | Feces, fecal swab, oral fluid |
Success rate: PSaV screening PCR with a ct of 30 or lower. Cost: VP1 PCR - $50.00 Sequencing and analysis - $100.00 Total cost = $150.00
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL.
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| Histopathology | All Species | IHC | $20.00 | as needed | tissue, unstained slide | ||||
| Bovine viral diarrhea virus - BVDV | Bovine | IHC | $20.00 | as needed | lung, spleen, thymus |
For diagnostic cases only
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| Actinobacillus pleuropneumoniae - APP | Porcine | CF Serotype 1, 5, 7 | $15.00 | $13.50 | as needed | 2-day test | serum | ||
| Influenza A virus - IAV | Porcine | IHC | $20.00 | as needed | lung | ||||
| NGS^ | All Species | Novel/uncharacterized pathogen detection | $400.00 | as needed | 3-4 weeks | infected clinical/body fluids, tissues, feces |
$400.00/library/sample
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| Mycoplasma bovis | Bovine | IHC | $20.00 | as needed | lung | ||||
| Equine herpesvirus type 1- EHV1 | Equine | VI | $25.00 | as needed | 7-14 days | fetal tissues (lung, liver, thymus, spleen, placenta), lung, blood, nasal swab, brain | |||
| NGS^ | All Species | Mixed infection detection | $400.00 | as needed | 3-4 weeks | infected clinical/body fluids, tissues, feces |
$400.00/library/sample
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| Porcine parvovirus type 2 - PPV2^^ | Porcine | PCR (R&D) | $30.00 | as needed | next day | Porcine | |||
| Porcine epidemic diarrhea virus - PEDV | Porcine | VI | $30.00 | as needed | fecal swab, feces, intestine | ||||
| NGS^ | All Species | Viral whole genome sequencing | $300.00 | as needed | 3-4 weeks | cell culture; infected/clinical tissue, body fluids, and feces |
$300.00/cell culture isolate sample; $300.00/infected or clinical tissue sample; Ct <24
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| Porcine epidemic diarrhea virus - PEDV | Porcine | IHC | $20.00 | as needed | intestine | ||||
| Porcine sapovirus genogroup III - PSaV^^ | Porcine | PCR (R&D) | $30.00 | $27.00 | as needed | next day | Feces, fecal swab, oral fluid | ||
| Brachyspira spp. | Porcine | Sequencing (nox gene) | $50.00 | $45.00 | as needed | 2-3 days | identification from culture | ||
| NGS^ | All Species | Shotgun metagenomics sequencing | $800.00 | as needed | 3-4 weeks |
Provide 5-10Gb sequencing data for each sample; $800.00/sample ($650.00/sample if sample number is more than 96)
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| Porcine sapelovirus - PSV | Porcine | Virus isolation | $30.00 | as needed | 7 - 14 days | serum, brain, spinal cord, lymph node | |||
| Equine herpesvirus type 3 - EHV3 | Equine | VI | $25.00 | as needed | 7-14 days | reproductive tract scraping/swab | |||
| NGS^ | All Species | Bacterial genomic epidemiological analysis | $200.00 | as needed | 3-4 weeks | pure bacterial strains |
Identify bacterial virulome, resistome, and perform phylogenetic analysis based on maximum common genome sequences; $200.00/strain (Inquire if strain number is more than 96)
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| Equine herpesvirus type 4 - EHV4 | Equine | VI | $25.00 | as needed | 7-14 days | fetal tissues (lung, liver, thymus, spleen, placenta), lung, blood, nasal swab, brain | |||
| Lawsonia intracellularis | Equine, Porcine | IHC | $20.00 | as needed | colon, intestine | ||||
| Porcine parvovirus - PPV | Porcine | VI | $30.00 | as needed | 7-14 days | aborted fetal tissue, fetal thoracic fluid | |||
| Equine influenza virus - EIV | Equine | VI | $25.00 | as needed | 7-14 days | lung, nasal swab | |||
| Slide preparation without interpretation | All Species | Slide preparation | $10.00 | as needed | tissue |
Bulk rate: $5.50 for the first slide and $1.00 for each additional slide requested per sample
E.g.: 10 unstained slides from the same block = $5.50 + $9.00 = $14.50
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| Leptospira interrogans | All Species | IHC | $20.00 | as needed | kidney, liver | ||||
| Leptospira | All Species | Individual serovars | $6.00 | $5.40 | as needed |
bratislava, canicola, copenhageni, grippotyphosa, hardjo, ictero, pomona
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| Necropsy | All Species | Necropsy - General | $50.00 | as needed |
Base fee for all non-avian, non-fetal necropsy cases. For cases including more than 3 juvenile to adult animals, additional fees may apply.
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| Porcine circovirus type 2 - PCV2 | Porcine | IHC | $20.00 | as needed | kidney, lung, lymph node | ||||
| West Nile virus - WNV | All Species | IHC | $20.00 | as needed | brain | ||||
| Necropsy | Avian | Necropsy - Avian adult (each) | $10.00 | as needed | |||||
| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | IFA European-titration | $15.00 | $13.50 | as needed | same day | serum | ||
| Porcine deltacoronavirus - PDCoV^^ | Porcine | VI (R&D) | $30.00 | as needed | 7-21 | fecal swab, feces, intestine | |||
| Erysipelothrix rhusiopathiae | All Species | IHC | $20.00 | as needed | skin, spleen | ||||
| Porcine reproductive & respiratory syndrome virus - PRRSV^^ | Porcine | IgA (R&D) | $9.00 | as needed | |||||
| Pseudorabies - PRV | Porcine | ELISA g1 | $5.50 | $4.95 | as needed | same day | serum, meat juice | ||
| Listeria monocytogenes | Bovine, Caprine, Ovine, Porcine | IHC | $20.00 | as needed | brain, placenta | ||||
| Necropsy | Avian | Necropsy - Avian juvenile (each) | $5.00 | as needed | |||||
| Canine adenovirus- CAdV | Canine | VI | $25.00 | as needed | 7-14 days | lung, feces, intestine, nasal swab | |||
| Biopsy | All Species | Biopsy | $60.00 | as needed |
Up to 5 slides per animal ( each additional slide $5.00)
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| Necropsy | Porcine | Necropsy - Swine fetal | $5.00 | as needed | |||||
| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | IFA North American -titration | $15.00 | $13.50 | as needed | same day | serum | ||
| Necropsy | All Species | Necropsy - General >3 animals (each) | $25.00 | as needed |
Fee for all animals beyond the first 3 on a case.
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| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | Homologous IFA- screen @ 1:20 | $5.00 | $4.50 | as needed | 2-4 weeks | serum |
call office
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| Pseudorabies - PRV | Porcine | VI | $25.00 | as needed | 7-14 days | brain, lung, tonsil | |||
| Feline calicivirus - FCV | Feline | VI | $25.00 | as needed | 7-14 days | lung, nasal swab, conjunctival swab | |||
| Necropsy | Equine | Disposal - Equine | $300.00 | as needed |
$300 for >200# by landfill; Free for <200# by incineration
This is for diagnostic cases only.
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| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | Homologous IFA - titration | $15.00 | $13.50 | as needed | 2-4 weeks | serum |
Call office 515-294-1950
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| Porcine circovirus type 2 - PCV2 | Porcine | VI | $30.00 | as needed | 7-14 days | serum, lung, lymph nodes, tonsil | |||
| Canine Parvovirus - CPV | Canine | Sequencing (VP1) | $150.00 | $120.00 | as needed | 3-5 days | Feces, intestine, fecal swab, intestinal content |
A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL.
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| Canine herpesvirus - CHV | Canine | VI | $25.00 | as needed | 7-14 days | lung, kidney, liver, nasal swab | |||
| Porcine reproductive & respiratory syndrome virus - PRRSV | Porcine | IHC | $20.00 | as needed | lung, lymph node | ||||
| Pullorum | Avian | AGG | $3.00 | $2.70 | as needed | same day | serum, whole blood | ||
| Feline herpesvirus - FHV | Feline | VI | $25.00 | as needed | 7-14 days | lung, nasal swab, conjunctival swab | |||
| Neospora | All Species | IHC | $20.00 | as needed | Brain, placenta, muscle | ||||
| Canine parvovirus - CPV | Canine | VI | $25.00 | as needed | 7-14 days | intestine, feces | |||
| Feline parvovirus-FPV | Feline | VI | $25.00 | as needed | 7-14 days | intestine, feces | |||
| Bovine parainfluenza virus type 3 - BPIV3 | Bovine | VI | $25.00 | as needed | 7-14 days | lung, nasal swab | |||
| Interpretation - Diagnostic | All Species | Interpretation | $25.00 | as needed |
per clinical diagnostic case investigation on all submissions with gross pathology and/or histopathologic examination or tissues along with other ancillary diagnostic testing (additional fee of $5.00 per slide if more than 10 slides)
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| Porcine teschovirus - PTV | Porcine | VI | $30.00 | as needed | 7-14 days | spinal cord, brain | |||
| Mycoplasma hyopneumoniae - MHP | Porcine | IHC | $20.00 | as needed | lung | ||||
| Interpretation - Research | All Species | Interpretation | $150.00 | as needed |
Fee is assessed per hour in 30 minute increments.
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| Block preparation & return | All Species | Block preparation | $5.50 | as needed |
Paraffin-embedded block preparation & return only
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| Rotavirus group A | Bovine, Equine, Porcine | IHC | $20.00 | as needed | intestine | ||||
| Histopathology | All Species | Special stains | $15.00 | as needed | tissue, unstained slide | ||||
| Slide preparation with existing block | All Species | Slide preparation | $5.50 | as needed |
Per slide from existing paraffin block & return only
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| Senecavirus A - SVA (Seneca Valley virus)^ | Porcine | Sequencing (VP1) | $150.00 | $135.00 | as needed | 3-5 days | serum, tissue (vesicles), vesicular fluid, vesicular swab, oral fluid |
PCR - SVA VP1 = $50; Sequencing and Analysis = $100
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Infectious bovine rhinotracheitis virus - IBRV | Bovine, Caprine, Ovine | IHC | $20.00 | as needed | tissue | ||||
| Slide preparation with interpretation | All Species | Slide preparation | $7.00 | as needed |
Per slides on cases with histopathologic interpretation at ISU VDL
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| Senecavirus A - SVA (Seneca Valley virus) | Porcine | VI | $30.00 | as needed | 7-14 days | serum, tissue (vesicles), vesicular fluid, oral fluid | |||
| Bovine respiratory syncytial virus - BRSV | Bovine | Virus isolation | $30.00 | as needed | one week | Lung, nasal swab, oropharyngeal swab | |||
| Bovine respiratory syncytial virus - BRSV | Bovine, Caprine, Ovine | IHC | $20.00 | as needed | lung | ||||
| Porcine reproductive & respiratory syndrome virus - PRRSV^^ | Porcine | IgM (R&D) | $9.00 | as needed | oral fluids, serum | ||||
| Porcine parainfluenza virus type 1 - PPIV-1 | Porcine | ELISA (R&D) | $7.50 | $6.25 | as requested | same day | serum | ||
| Virus growth | All Species | Virus propagation | $30.00 | contact lab | |||||
| Virus quantitation | All Species | Virus quantitation | $120.00 | contact lab | contact lab | ||||
| Rabies | All Species | Emergency(weekend and after-hours) | $130.00 | contact lab | within 48 hours | brain | |||
| Porcine rotavirus | Porcine | PCR | $30.00 | $27.00 | Daily | Next day | Feces, intestine, fecal swab, oral fluid | ||
| Toxo - Toxoplasma gondii | All Species | ELISA | $25.00 | $22.50 | F | same day | serum | ||
| Porcine deltacoronavirus - PDCoV^^ | Porcine | S1 IgG ELISA (R&D) | $7.50 | F | same day | serum | |||
| Bovine respiratory syncytial virus - BRSV | Bovine | VN | $5.00 | F | following Friday | serum | |||
| Influenza A virus - IAV | Porcine | NP ELISA | $5.00 | $4.50 | F | same day | serum | ||
| Transmissible gastroenteritis virus - TGEV | Porcine | VN | $5.00 | F | following Wednesday | serum | |||
| Bovine viral diarrhea virus-BVDV | Bovine | VN- type I | $5.00 | F | following Wednesday | serum | |||
| Bovine viral diarrhea virus-BVDV | Bovine | VN- type II | $5.00 | F | following Wednesday | serum | |||
| Mycobacterium paratuberculosis (Johne's) | Bovine | CF | $6.00 | $5.40 | F | same day | serum | ||
| Pseudorabies - PRV | Porcine | VN | $5.00 | F | following Monday | serum | |||
| Vesicular stomatitis - VS | Bovine, Camelid, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates | VN | $5.00 | F | following Monday | serum |
Indiana or New Jersey Serotype (please specify) (Bison)
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| Canine herpesvirus - CHV | Canine | VN | $5.00 | F | following Monday | serum | |||
| Coxiella burnetii (Q-Fever) | Bovine, Caprine | IFA | $15.00 | $13.50 | F | same day | serum | ||
| Bovine parainfluenza virus type 3 - BPIV3 | Bovine | VN | $5.00 | F | M | serum | |||
| Caprine arthritis encephalitis virus-CAE | Caprine, Ovine | cELISA | $6.50 | $5.85 | F | same day | serum | ||
| Infectious bovine rhinotracheitis virus - IBR | Bovine | VN | $5.00 | F | following Monday | serum | |||
| NGS^ | All Species | 16s metagenomics analysis | $150.00 | Inquiry | environmental samples, infected/clinical body fluids, tissues, and feces |
Please call the lab for additional information.
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| Actinobacillus pleuropneumoniae - APP Serotyping Detects serotypes 1, 2, 3, 5, 6, 7, 8, 10, 12 | Porcine | PCR - serotyping | $60.00 | $54.00 | M | next day | culture | ||
| Influenza A virus - IAV | Porcine | HI H1 - H1 gamma '99 | $5.00 | M | Tu | serum | |||
| Influenza A virus - IAV | Porcine | HI H3 - H3 Cluster 4 | $7.00 | M | Tu | serum | |||
| Glaesserella parasuis - GPS | Porcine | PCR - serotype | $60.00 | $54.00 | M | next day | Pure Culture | ||
| Actinobacillus pleuropneumoniae - APP | Porcine | Mix APP ELISA | $12.00 | $10.80 | M | same day | serum |
MIX APP ELISA generates 3 results for these serotype groups: 1-2-9-11, 3-6-8-15 and 4-5-7
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| Influenza A virus - IAV | Porcine | HI Zoetis Ags (4) | $28.00 | M | Tu | serum | |||
| Senecavirus A - SVA (Seneca Valley virus) | Porcine | IFA Screen | $9.00 | M | F | Serum | |||
| Porcine epidemic diarrhea virus - PEDV | Porcine | IFA screen | $5.50 | M | F | serum | |||
| Influenza A virus - IAV | Porcine | HI, H3 Cluster 1/3 | $7.00 | M | Tu | serum | |||
| Porcine epidemic diarrhea virus - PEDV | Porcine | IFA 4 dilution | $15.00 | M | F | serum | |||
| Influenza A virus - IAV | Porcine | HI, H1 Classical '73 | $5.00 | M | Tu | serum | |||
| Porcine parvovirus - PPV | Porcine | HI | $7.00 | M | Tu | serum | |||
| Influenza A virus - IAV | Avian, Bovine, Canine, Equine, Feline, Porcine | HI, H1 pandemic | $8.00 | M | Tu | serum | |||
| Senecavirus A - SVA (Seneca Valley virus) | Porcine | 4-dil IFA | $15.00 | M | F | serum | |||
| Porcine epidemic diarrhea virus - PEDV^^ | Porcine | S1 FMIA IgA (R&D) | $9.00 | M | same day | Serum, colostrum, milk, oral fluids |
add $1 if colostrum or milk to cover cost of processing
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| Porcine circovirus type 2 - PCV2 | Porcine | FFN | $16.00 | M | F | serum | |||
| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | FFN | $15.00 | M | F | serum | |||
| Porcine epidemic diarrhea virus - PEDV | Porcine | HTNT | $16.00 | M | F | serum | |||
| Erysipelothrix rhusiopathiae - ERY | Porcine | FMIA Luminex | $6.00 | $5.40 | M | same day | serum | ||
| Hemorrhagic Enteritis Virus | Avian | ELISA | $2.50 | $2.25 | M | Same day | Serum |
Turkey only
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| Erysipelothrix spp. | Porcine | PCR E rhusiopathiae, E. tonsilarum, E. sp2 | $30.00 | $27.00 | M | next day | joint fluid, skin, spleen | ||
| Bordetella Avium E | Avian | ELISA | $2.50 | $2.25 | M | Same day | Serum |
Turkey only
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| Actinobacillus pleuropneumoniae - APP | Porcine | ELISA 10-12 | $4.00 | $3.60 | M | same day | serum | ||
| Bluetongue - BT | Bovine, Caprine, Cervidae, Ovine, Ungulates | cELISA | $8.00 | $7.20 | M | Same day | serum | ||
| Mycoplasma spp - eMyco^ | All Species | PCR with Sequencing | $60.00 | $54.00 | M | next day | lung, oral fluid, bacterial isolate |
Cost: $30 for PCR, $30 for sequencing. Total cost=$60
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Mycoplasma gallisepticum - MG | Avian | ELISA | $2.50 | $2.25 | M | same day | serum |
chicken, turkey
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| Mycoplasma synoviae - MS | Avian | ELISA | $2.50 | $2.25 | M | same day | serum |
chicken, turkey
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| Influenza A Virus - IAV | Porcine | HI H1N2 human-like | $7.00 | M | Tu | serum | |||
| Porcine reproductive & respiratory syndrome virus - PRRSV^^ | Porcine | IgM/IgA ELISA (R&D) | $9.00 | M | same day | oral fluids | |||
| Leptospira | All Species | PCR | $30.00 | $27.00 | M - F | same day | kidney, urine | ||
| Leptospira hardjo bovis | Bovine | PCR | $30.00 | $27.00 | M - F | same day | kidney, urine | ||
| Mycoplasma synoviae – M. synoviae | Avian | PCR | $30.00 | $27.00 | M - F | same day | Oropharyngeal swab, tracheal swab | ||
| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | VI | $25.00 | M, F | 7-14 days | serum, oral fluid, lung | |||
| Porcine sapelovirus - PSV | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | spinal cord, brain, feces, fecal swab, oral fluid | ||
| Escherichia coli - E. coli | All Species | PCR -geno typing | $45.00 | $40.50 | M, Th | next day | culture |
E. coli genotyping PCR targets the following genes:
Toxin Genes: EAST1, LT, Sta, STb, Stx1, Stx2, Stx2e;
Pilus Genes: F18, F41, K88, K99, 987P;
Adhesin Genes: AIDA, EAEA, PAA
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| Influenza B virus - IBV^^ | Bovine, Porcine | PCR (R&D) | $30.00 | $27.00 | M, Th | next day | lung, nasal swab, pharyngeal swab | ||
| Actinobacillus pleuropneumoniae - APP | Porcine | PCR - screen | $30.00 | $27.00 | M, Th | next day | culture, lung, oral fluid | ||
| Influenza C Virus - ICV^^ | Bovine, Porcine | PCR (R&D) | $30.00 | $27.00 | M, Th | next day | lung, nasal swab, pharyngeal swab | ||
| Equine adenovirus 1 - EAdV1 | Equine | PCR | $30.00 | $27.00 | M, Th | next day | nasal swab, lung, BAL | ||
| Actinobacillus suis - A. suis | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | lung, bronchial swab, culture, laryngeal swab, oral fluid, pharyngeal swab | ||
| Equine herpesvirus type2 - EHV2 | Equine | PCR | $30.00 | $27.00 | M, Th | next day | nasal swab, lung | ||
| Ureaplasma | Bovine | PCR | $35.00 | $31.50 | M, Th | next day | lung, placenta, vulvovaginal swab | ||
| Equine herpesvirus type 3 - EHV3 | Equine | PCR | $30.00 | $27.00 | M, Th | next day | vaginal swab | ||
| Anaplasma marginale - Ana | Bovine | PCR | $30.00 | $27.00 | M, Th | next day | whole blood (EDTA), spleen | ||
| Avian hepatitis E virus - aHEV | Avian | PCR | $30.00 | $27.00 | M, Th | next day | liver, feces, fecal swab, intestinal content, intestine | ||
| Porcine parvovirus - PPV | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | fetal tissue | ||
| Avian reovirus - AvRV | Avian | PCR | $30.00 | $27.00 | M, Th | Same day | tendons | ||
| Avian paramyxovirus-1 - aPMV-1 | Avian | VI | $50.00 | M, Th | 7-21 days | cloacal swab, oropharyngeal swab, feces, tracheal swab, environmental | |||
| Brachyspira hyodysenteriae/hampsonii | Porcine | PCR | $45.00 | $40.50 | M, Th | next day | feces, fecal swab, culture |
Duplex real-time PCR intended for diagnosing swine dysentery (SD) associated with Brachyspira hyodysenteriae or Brachyspira hampsonii infections in swine.
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| Avian Infectious Bronchitis Virus - AvIBV | Avian | PCR | $30.00 | $27.00 | M, Th | Same day | Lungs, trachea | ||
| Avian Infectious Laryngotracheitis Virus - AvILTV | Avian | PCR | $30.00 | $27.00 | M, Th | Same day | Lungs, trachea | ||
| Avian ornithobacterium rhinotracheale - AvORT | Avian | PCR | $30.00 | $27.00 | M, Th | Same day | Lungs, trachea | ||
| Avian influenza virus - AIV | Avian | VI | $50.00 | M, Th | 7-21 days | cloacal or oropharyngeal swab, feces, oral+cloacal, tracheal swab, environmental sample | |||
| Canine adenovirus- CAdV | Canine | PCR | $30.00 | $27.00 | M, Th | next day | liver, laryngeal swab, tracheal swab, lung, nasal swab | ||
| Malignant catarrhal fever (MCF) | Bovine, Caprine, Cervidae, Ovine, Porcine | PCR | $30.00 | $27.00 | M, Th | next day | nasal swab, lung, brain | ||
| Canine coronavirus - CCoV | Canine | PCR | $30.00 | $27.00 | M, Th | next day | feces, fecal swab | ||
| Feline calicivirus - FCV | Feline | PCR | $30.00 | $27.00 | M, Th | next day | lung, nasal swab, conjunctival swab | ||
| Canine influenza virus - CIV | Canine | PCR - Subtype | $35.00 | $31.50 | M, Th | next day | lung, BAL, nasal swab | ||
| Canine distemper virus - CDV | Canine | PCR | $30.00 | $27.00 | M, Th | next day | lung, brain, nasal swab | ||
| Streptococcus suis (S. suis) | Porcine | PCR | $30.00 | $27.00 | M, Th | same day | brain, lung, fibrin, heart, joint fluid | ||
| Mycotoxins panel | All Species | LC-MS/MS | $100.00 | M, Th | 3-5 days | corn, grain, feed, mixed feed (1lb) |
Comments: Panel includes: Aflatoxin B1, B2, G1, G2, Dexoynivalenol, 3-Acetyl Deoxynivalenol, Fumonisin B1, B2, Ochratoxin A, T-2, HT-2, Zearalenone, α Zearalenol. See our mycotoxin website page for more info.
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| Canine Parvovirus - CPV | Canine | PCR - VP1 | $65.00 | $58.50 | M, Th | 3-5 days | Feces, intestine, fecal swab, intestinal content | ||
| Canine herpesvirus - CHV | Canine | PCR | $30.00 | $27.00 | M, Th | next day | lung, nasal swab, kidney, liver | ||
| Feline herpesvirus - FHV | Feline | PCR | $30.00 | $27.00 | M, Th | next day | lung, nasal swab, conjunctival swab | ||
| Porcine kobuvirus - PKV | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | feces, fecal swab, oral fluid | ||
| Encephalomyocarditis virus – EMCV^^ | Porcine | PCR (R&D) | $30.00 | $27.00 | M, Th | next day | brain, spinal cord, heart | ||
| Porcine cytomegalovirus - PCMV | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | nasal swab, turbinates, lung | ||
| Porcine astrovirus type 3 – PoAstV3 | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | Spinal cord, brain, feces, virus isolate | ||
| Mycoplasma flocculare | Porcine | PCR | $25.00 | $22.50 | M, Th | next day | lung, nasal swab, culture | ||
| Bovine parainfluenza virus type 3 - BPIV3 | Bovine | PCR | $30.00 | $27.00 | M, Th | next day | BAL, lung, nasal swab, tracheal wash | ||
| Feline panleukopenia virus - FPLV | Feline | PCR | $30.00 | $27.00 | M, Th | next day | intestine, feces, fecal swab | ||
| Chlamydia suis – C. suis^^ | Porcine | PCR (R&D) | $30.00 | $27.00 | M, Th | next day | Eye swab | ||
| Porcine teschovirus – PTV | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | spinal cord, brain, feces, fecal swab, oral fluid |
This assay detects all serotypes of PTV (1-7, 11-13).
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| Coxiella burnetii | Caprine, Ovine | PCR | $30.00 | $27.00 | M, Th | next day | feces, milk, placenta, vaginal swab, stomach content | ||
| Canine parvovirus-2 - CPV-2 | Canine | PCR | $30.00 | $27.00 | M, Th | next day | intestine, feces, fecal swab | ||
| Porcine hemagglutinating encephalomyelitis virus - PHEV | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | brain, spinal cord | ||
| Glaesserella parasuis - GPS | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | lung, BAL, bronchial swab, joint fluid, heart, fibrin, oral fluid, pleural swab | ||
| Caprine herpesvirus - CpHV | Caprine | PCR | $30.00 | $27.00 | M, Th | next day | lung, nasal swab, vaginal swab, lymph node, BAL | ||
| Pseudorabies - PRV | Bovine, Canine, Cervidae, Porcine | PCR | $30.00 | $27.00 | M, Th | next day | brain, tonsil, lung, fetal tissues | ||
| Mycoplasma hyorhinis - MHR | Porcine | PCR | $25.00 | $22.50 | M, Th | next day | lung, oral fluid, joint swab, joint fluid, fibrin, heart | ||
| Mycoplasma hyosynoviae - MHS | Porcine | PCR | $25.00 | $22.50 | M, Th | next day | joint swab, joint fluid, oral fluids, lung, tonsil | ||
| Pestivirus - Atypical Porcine Pestivirus/Congenital Tremor (APPV) | Porcine | PCR - Quantitative | $32.00 | $28.80 | M, Th | next day | serum, brain, fecal swab, nasal swab, heart | ||
| Neospora | Bovine | PCR | $30.00 | $27.00 | M, Th | next day | Brain, liver, heart | ||
| West nile virus - WNV | Avian, Equine | PCR | $30.00 | $27.00 | M, Th | next day | brain, serum |
WNV PCR based on species:
1) Mosquitoes: mosquito pools
2) Birds: Serum and bird tissues
3) Horses: Preferable brain and spinal cord; Cerebrospinal fluid is acceptable
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| Mycoplasma suis - M. Suis (formerly Eperythrozoon suis) | Porcine | PCR | $30.00 | $27.00 | M, Th | next day | whole blood (EDTA or heparin), spleen | ||
| Swine hepatitis E virus - sHEV^^ | Porcine | PCR (R&D) | $35.00 | $31.50 | M, Th | next day | liver | ||
| Mycoplasma | Porcine | Culture | $40.00 | M, Tu, F |
M - Friis media set-up, Tu & F - BHI & Difco media set-up; Turn around time: 4 weeks for M. hyopneumoniae, 17 days for M. hyorhinis or M. hyosynoviae
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| Bovine viral diarrhea virus-BVDV | Bovine | VI | $25.00 | M, W | 7-10 days | blood, lung, serum, semen | |||
| Porcine deltacoronavirus - PDCoV^ | Porcine | Sequencing, S gene | $150.00 | $135.00 | M, W, F | 3-5 days | Feces, fecal swab, oral fluid |
Sample amount: no less than 1mL fluid or 3 grams tissue Success rate: PDCoV PCR screening with a ct of 22 or lower is recommended Cost: PDCoV S Gene PCR- $50.00, PDCoV sequencing and analysis- $100.00: Total cost=$150.00 ^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Influenza A virus - IAV^ | Canine, Equine, Feline, Porcine | Sequencing (hemagglutinin (HA), ~1.7kb) | $110.00 | $99.00 | M, W, F | up to two business weeks from time of request | BAL, lungs, nasal swabs, oral fluid, bronchial swabs and virus isolates |
 USDA IAV.pdf |
USDA surveillance samples may be delayed while the required isolate is obtained.
Sample amount: no less than 1mL fluid, 3 grams of tissue
Success rate: IAV screening PCR with a ct of 35 or lower for lung, BAL and nasal swab and a ct of 30 or lower for oral fluids is recommended.
Cost: IAV HA gene PCR-$35.00
IAV Sequencing and analysis- $75.00
Total cost=$110.00
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
USDA IAV.pdf |
| Porcine circovirus type 3 - PCV3^ | Porcine | Sequencing (OFF2) | $150.00 | $135.00 | M, W, F | 3-5 days | lung, umbilicus, oral fluid, serum, processing fluid, fetal tissue |
Sample amount: no less than 1mL fluid or 3 grams of tissue.
Success rate: PCV3 PCR screening with a ct of 32 or lower is recommended.
Cost: PCV ORF2 PCR - $50.00, PCV sequencing and analysis - $100.00: Total cost=$150.00
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Porcine epidemic diarrhea virus - PEDV^ | Porcine | Sequencing (S1 portion, ~2.2kb) | $150.00 | $135.00 | M, W, F | 3-5 days | feces, fecal swab, intestines, oral fluids, environmentals |
Sample amount: no less than 1mL fluid or 3 grams tissue
Success rate: PEDV PCR screening with a ct of 21 or lower is recommended
Cost: PEDV S1 PCR- $50.00, PEDV sequencing and analysis- $100.00: Total cost=$150.00
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Porcine parainfluenza virus type 1 – PPIV-1^ | Porcine | Sequencing (F gene) | $150.00 | $135.00 | M, W, F | 3-5 days | lung, nasal swabs, oral fluids, BAL, bronchial swab |
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Porcine circovirus type 2 - PCV2^ | Porcine | Sequencing (ORF2) | $150.00 | $135.00 | M, W, F | 3-5 days | lungs, lymph nodes, oral fluid, serum, processing fluid |
Sample amount: no less than 1mL fluid or 3 grams of tissue.
Success rate: PCV2 PCR screening with a ct of 32 or lower is recommended.
Cost: PCV ORF2 PCR - $50.00, PCV sequencing and analysis - $100.00: Total cost=$150.00
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Mycoplasma hyopneumoniae - MHP^ | Porcine | Sequencing (P146 adhesion gene) | $145.00 | $130.50 | M, W, F | lung, bronchial swab, BAL, oral fluids, nasal swab, laryngeal swab, tracheal swab |
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL
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| Porcine rotavirus type A, B, & C^ | Porcine | PCR w/ Sequence | $145.00 | $130.50 | M, W, F | 3-5 days | feces, intestine, fecal swab, oral fluid |
Sample amount: no less than 1 ml fluid, 3 grams tissue
Success rate: rota screening PCR with a ct of 25 or lower is recommended
Cost: VP7 PCR $85.00 Sequencing and analysis $60.00
If sequencing not successful, cloning can be requested for an additional $70.00.
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| Porcine parainfluenza virus type 1 – PPIV-1^ | Porcine | Sequencing (HN gene) | $150.00 | $135.00 | M, W, F | 3-5 days | lung, nasal swabs, bronchial swab, BAL, oral fluids |
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL.
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| Porcine epidemic diarrhea virus / Porcine deltacoronavirus / Transmissible gastroenteritis virus - PEDV/PDCoV/TGEV | Porcine | PCR - Multiplex | $25.00 | $22.50 | M-F | same day | feces, fecal swabs, intestine, oral fluids, environmental samples | ||
| Clostridium sp. | All Species | FA | $10.00 | M-F | 1-2 days | muscle | |||
| Porcine circovirus type 2d - PCV2d^ | Porcine | PCR | $30.00 | $27.00 | M-F | same day | Lung, fetal tissue, oral fluid, brochoalveolar lavage, fecal swab, nasal swab, processing fluid | ||
| Coliform | All Species | Culture | $15.00 | M-F | 1-2 days | water | |||
| Bovine viral diarrhea virus-BVDV | Bovine, Caprine, Ovine | PCR - screen | $30.00 | $27.00 | M-F | same day | feces, intestinal content, fecal swab, buffy coat, intestine, lung, lymph node, semen, serum, spleen, milk, fresh ear notch | ||
| Mycoplasma iowae - AvMI | Avian | PCR | $30.00 | $27.00 | M-F | same day | oropharyngeal swab, tracheal swab | ||
| Transmissible gastroenteritis virus - TGEV | Porcine | PCR | $25.00 | $22.50 | M-F | same day | feces, fecal swab, intestine, environmental, oral fluid | ||
| Bovine viral diarrhea virus - BVDV | Bovine | IHC (1-3 notches) | $20.00 | $18.00 | M-F | next day | formalin-fixed ear notches |
For surveillance cases only.
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| Mycoplasma meleagridis - AvMM | Avian | PCR | $30.00 | $27.00 | M-F | same day | oropharyngeal swab, tracheal swab | ||
| Influenza A virus - IAV | Porcine | PCR - Screen & Subtype ALL USDA | $35.00 | $31.50 | M-F | lung, nasal swab, oral fluids |
Includes subtyping of all positive samples per case. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory.
Acceptable swabs:
Star Swab II, Copan swabs, Other swabs are also available.
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| Porcine circovirus type 2 & 3 - PCV2/3 | Porcine | PCR | $35.00 | $31.50 | M-F | same day | serum, oral fluid, lung, lymph node, processing fluid | ||
| Porcine reproductive & respiratory syndrome virus- PRRSV^ | Porcine | BIVI Ingelvac MLV CLAMP Sequencing (ORF5 region, ~600bp) | $220.00 | $198.00 | M-F | 2-4 days | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
Sample amount: no less than 1 mL fluid, 3 grams of tissue.
Success rate: PRRSV screening PCR with a a ct of 30 or lower is recommended.
Cost: ORF5 CLAMP PCR - $90.00 Sequencing and analysis - $65 per sequence recovered, maximum of 2. Total maximum cost=$220.
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| Equine arteritis virus - EAV | Equine | PCR | $30.00 | $27.00 | M-F | same day | nasal swab, EDTA blood, lung, semen, fetal tissues (lung, liver, kidney, thymus, spleen, placenta) | ||
| Trichomonas | Bovine | Bacterial culture | $10.00 | M-F | 1 week | trich pouch |
If culture is desired samples need to arrive at the laboratory Monday - Friday during business hours. All overnight / refrigerated sample will be tested via PCR.
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| Influenza A virus - IAV | Canine, Equine, Feline, Porcine | PCR - Screen and Subtype (positives) | $35.00 | $31.50 | M-F | screen - same day; subtype - next day | lung, nasal swab, BAL, oral fluids, bronchial swab, allantoic fluid, feces, oropharyngeal swab, pharyngeal swab, tracheal swab |
Includes subtyping all positive samples. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory.
Acceptable swabs:
Star Swab II, Copan swabs, Other swabs are also available.
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| Porcine hemagglutinating encephalomyelitis virus - PHEV^^ | Porcine | IgG ELISA (R&D) | $7.00 | M-F | same day | serum, plasma | |||
| Parasitology | All Species | Direct Smear | $15.00 | M-F | next business day | feces |
If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
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| Equine herpesvirus type1&4 - EHV1&4 | Equine | PCR | $35.00 | $31.50 | M-F | same day | nasal swab, lung, brain, fetal tissues (lung, liver, kidney, thymus, spleen, placenta) buffy coat | ||
| Tritrichomonas foetus | Bovine, Feline | PCR | $25.00 | $22.50 | M-F | same day | inoculated trich pouch (InPouch), preputial wash, feline-feces only |
Sample Collection Instructions |
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| Influenza A virus - IAV | Canine, Equine, Feline, Porcine | PCR - Screen only (no subtype) | $25.00 | $22.50 | M-F | screen - same day; subtype - next day | lung, nasal swab, BAL, oral fluids, bronchial swab, allantoic fluid, feces, oropharyngeal swab, pharyngeal swab, tracheal swab |
Follow up IAV subtyping added on after initial testing $30 per sample.
Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory.
Acceptable swabs:
Star Swab II, Copan swabs, Other swabs are also available.
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| Brachyspira | Avian, Porcine | Culture | $15.00 | M-F | 1-2 weeks | Colon, feces, or fecal swabs |
Due to inherently high level of background flora in fecal samples, ISU VDL Bacteriology Section does not pool them for Brachyspira culture. Such samples are set up individually, reported individually, and charged individually.
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| Parasitology | All Species | Fecal flotation | $20.00 | M-F | next business day | feces (3-5 grams) |
If submitting greater than 10 fecal
samples, please contact the laboratory for scheduling
and approximate turnaround time.
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| Influenza A virus - IAV | Porcine | PCR - Screen & Subtype 1 USDA | $30.00 | $27.00 | M-F | lung, nasal swab, oral fluids |
Includes subtyping one positive sample per case. Appropriate swabs to use for influenza virus PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory.
Acceptable swabs:
Star Swab II, Copan swabs, Other swabs are also available.
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| Porcine epidemic diarrhea virus - PEDV | Porcine | PCR - Singleplex | $25.00 | $22.50 | M-F | same day | feces, fecal swabs, intestine, oral fluids, environmental samples | ||
| Parasitology | All Species | McMaster floatation | $25.00 | M-F | next business day | feces (1-2 grams) |
If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
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| Brucella | Bovine, Canine, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates | B. abortus/suis BAPA Screen | $1.00 | $0.90 | M-F | same day | serum |
Bison
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| Parasitology | All Species | Modified Wisconsin - quantitative | $30.00 | M-F | next business day | feces |
Due to the sensitivity of this test, all samples are screened first with a sugar floatation, if negative or if low numbers of oocysts are detected, the Modified Wisconsin method will then be performed for quantification. If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
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| Brucella | Bovine, Canine, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates | B. abortus/suis Card | $2.00 | $1.80 | M-F | same day | serum |
Unless specified by export, all swine sera submitted for the Card test will be tested for Brucellosis on the BAPA
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| Senecavirus A - SVA (Seneca Valley virus) | Porcine | PCR | $30.00 | $27.00 | M-F | same day | serum, tissue (vesicles), oral fluid, vesicular fluid and vesicular swabs |
Contact your state health official first if lesions suggest a foreign animal disease.
Appropriate swabs to use for SVA PCR are synthetic (Dacron or Rayon) tipped swabs on plastic shafts. Swabs should be placed in 1 ml of PBS in a falcon snap cap tube and the shaft broken low enough to allow the cap to be securely snapped tightly and avoid leakage. Do not send swabs in agar or gel-type media. Avoid using any calcium-alginate-tipped swabs or bleach. Also avoid cotton swabs with wooden shafts. Ship swabs overnight to the diagnostic laboratory. Acceptable swabs: Star Swab II, Copan swabs, Other swabs are also available.
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| Brucella | Bovine, Porcine | B. abortus FPA | $7.50 | $6.75 | M-F | same day | serum | ||
| Porcine circovirus type 1 - PCV1 | Porcine | PCR - PCV1 | $30.00 | $27.00 | M-F | same day | serum, oral fluid, processing fluid | ||
| Equine infectious anemia - EIA | Equine | AGID | $6.00 | $5.40 | M-F | next day | serum | ||
| Pestivirus - Atypical Porcine Pestivirus/Congenital Tremor (APPV) | Porcine | PCR | $30.00 | $27.00 | M-F | same day | serum, brain, fecal swab, nasal swab, heart |
more info |
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| Brucella | Bovine, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates | B. abortus/suis RIV | $1.00 | $0.90 | M-F | same day | serum |
Bison
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| Brachyspira | Avian, Bovine, Canine, Porcine | Susceptibility | $40.00 | M-F | 7 days | feces, colon contents, oral fluids | |||
| Equine infectious anemia - EIA | Equine | ELISA | $6.00 | $5.40 | M-F | same day | serum | ||
| Mycobacterium paratuberculosis (Johne's) | Bovine, Caprine, Ovine | PCR | $30.00 | $27.00 | M-F | same day (Up to next day) | feces, fecal swab |
An extra day may be necessary as large herd surveillance cases need extra organization and oddly packaged samples (gloves/bags) require additional bio-security measures.
Please visit https://vetmed.iastate.edu/vdpam/about/FSVM/beef/bovine-disease-topics/johnes-disease for more information.
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| Avian influenza virus - AIV | Avian | ELISA | $3.00 | $2.70 | M-F | same day | chicken serum |
chicken serum is the only valid sample type
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| Lawsonia intracellularis - ileitis | Equine, Porcine | PCR | $30.00 | $27.00 | M-F | same day | feces, fecal swab, intestine | ||
| Brucella | Canine | B. canis RSAT | $10.00 | $9.00 | M-F | same day | serum | ||
| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | ELISA X3 | $5.00 | $4.50 | M-F | same day | serum, plasma | ||
| Direct Smear | All Species | $10.00 | M-F | 1-2 days | |||||
| Avian influenza virus - AIV | Avian | PCR | $30.00 | $27.00 | M-F | same day | cloacal swab, oropharyngeal swab, tissue samples | ||
| Porcine reproductive & respiratory syndrome virus - PRRSV | Porcine | PCR - BIVI Ingelvac ATP-like | $35.00 | $31.50 | M-F | Same day | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
This assay tests for the presence of vaccine virus in the samples but it is not a differential assay and does not exclude a potential co-infection with a wild-type PRRSV.
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| Brucella | Bovine, Caprine, Cervidae, Equine, Ovine, Porcine, Ungulates | Bruc. SPT | $2.50 | $2.25 | M-F | same day | serum |
Bison
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| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | ELISA OF | $7.50 | $6.75 | M-F | same day | oral fluids | ||
| Lawsonia intracellularis - Ileitis | Equine, Porcine | PCR - Quantitative | $32.00 | $28.80 | M-F | same day | feces, fecal swab, intestine | ||
| Equine rhinitis A and B virus - ERAV & ERBV | Equine | PCR | $35.00 | $31.50 | M-F | same day | nasal swab, lung | ||
| Bacterial isolation and identification - Swabs | Bovine, Porcine | Culture surveillance | $15.00 | M-F | 3 - 7 days | Swab |
Porcine nasal swabs are only effective for culturing Pasteurella and Bordetella. Nasal swabs are an unacceptable sample type for culturing other porcine respiratory pathogens. Fee is per swab.
For samples with inherently high level of background flora (e.g., fecal samples of any species, swine nasal/oral fluid samples), ISU VDL Bacteriology Section does not pool samples. Such types of samples are set up individually, reported individually, and charged individually.
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| Porcine reproductive & respiratory syndrome virus - PRRSV | Porcine | PCR - Zoetis Fostera-like | $35.00 | $31.50 | M-F | Same day | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
This assay tests for the presence of the vaccine virus in the sample but it is not a differential assay in that it does not exclude a potential co-infection with a wild-type PRRSV.
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| Erysipelothrix rhusiopathiae - ERY | All Species | Bacterial enrichment | $20.00 | M-F | 8 days |
Cost is in addition to routine culture on samples
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| Avian paramyxovirus-1 matrix gene - aPMV-1 | Avian | PCR | $30.00 | $27.00 | M-F | same day | cloacal swab, oropharyngeal swab, allantoic fluid, bronchial swab, pharyngeal swab, tracheal swab | ||
| Mycoplasma hyopneumoniae | Porcine | Titration | $100.00 | M-F | 4 weeks | Culture, challenge material |
$50.00 for each additional specimen beyond the first
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| Porcine circovirus type 3 – PCV3 | Porcine | PCR | $30.00 | $27.00 | M-F | same day | serum, oral fluid, lung, lymph node, processing fluid | ||
| Campylobacter | All Species | Culture | $10.00 | M-F | 1 week | ||||
| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | IFA European -screen @ 1:20 | $5.00 | $4.50 | M-F | same day | serum |
call office
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| Bacterial isolation and identification - Tissue | All Species | Culture | $25.00 | M-F | 3 - 7 days | Milk, Urine, Blood, Tissue |
$25.00 1st sample; $5.00 for each additional sample from the same animal. Large submissions with more than 10 samples may incur additional fees. Please contact the laboratory to discuss fees prior to submission of large sample sets.
For samples with inherently high level of background flora (e.g., fecal samples of any species, swine nasal/oral fluid samples), ISU VDL Bacteriology Section does not pool samples. Such types of samples are set up individually, reported individually, and charged individually.
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| Listeria | All Species | Enrichment culture | $35.00 | M-F | 14 weeks |
Cost is in addition to routine culture on samples
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| Porcine reproductive & respiratory syndrome virus - PRRSV | Porcine | PCR - PrimePac-like | $35.00 | $31.50 | M-F | Same day | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
This assay tests for the presence of vaccine virus in the samples but is not a differential assay in that is does not exclude a potential co-infection with a wild-type PRRSV.
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| Porcine circovirus type 2 - PCV2 | Porcine | PCR – detects genotypes PCV2a, PCV2b, PCV2c, PCV2d, PCV2e | $30.00 | $27.00 | M-F | same day | serum, oral fluid, lung, lymph node, processing fluid | ||
| Bacterial susceptibility testing | All Species | $25.00 | M-F | 1-2 days | |||||
| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | IFA North American -screen @ 1:20 | $5.00 | $4.50 | M-F | same day | serum | ||
| Porcine circovirus type 2 - PCV2 | Porcine | PCR - PCV2a/b: multiplex and differential | $30.00 | $27.00 | M-F | serum, lung, lymph node, oral fluid, processing fluid | |||
| Porcine reproductive & respiratory syndrome virus - PRRSV | Porcine | PCR - Elanco Prevacent-like | $35.00 | $31.50 | M-F | same day | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum | ||
| Salmonella (SE only) | Avian | Strip Test | $30.00 | $27.00 | M-F | minimum 3 days | Environmentals, Chick paper swabs, Chick papers, Eggs | ||
| Porcine circovirus type 2 - PCV2 | Porcine | PCR - Quantitative | $32.00 | $28.80 | M-F | same day | serum, lung, lymph node, oral fluid, processing fluid | ||
| Salmonella (SH) | Avian | Culture - SH | $15.00 | M-F | minimum 5 days | Environmentals, Chick paper swabs, Chick papers, Eggs |
This is an add-on fee who request both SE and SH testing.
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| Bluetongue and Epizootic Hemorrhagic Disease - BTV and EHD | Bovine, Camelid, Caprine, Cervidae, Ovine, Ungulates | AGID | $7.50 | $6.75 | M-F | next day | serum | ||
| Porcine reproductive & respiratory syndrome virus - PRRSV^^ | Porcine | Zoetis Fostera MLV CLAMP Sequencing - (R&D) | $220.00 | $198.00 | M-F | 2-4 days | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
Sample amount: no less than 1 mL fluid, 3 grams of tissue. Success rate: PRRSV screening PCR with a ct of 30 or lower is recommended. Cost: ORF5 CLAMP PCR - $90.00 Sequencing and analysis - $65 per sequence recovered, maximum of 2. Total maximum cost=$220.
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| Salmonella (All types) | Avian | Culture - All types | $30.00 | $27.00 | M-F | minimum 6 days | Chick Papers, DOA (Viscera), Feces, Feed | ||
| Mycoplasma | Avian, Bovine, Camelid, Canine, Caprine, Cervidae, Equine, Feline, Ovine, Rodent, Ungulates | Culture | $30.00 | M-F | 3 weeks | ||||
| Bovine coronavirus - BCoV | Bovine | PCR | $30.00 | $27.00 | M-F | same day | intestine, feces, fecal swab | ||
| Mycoplasma bovis | Bovine | PCR | $30.00 | $27.00 | M-F | same day | lung, BAL, milk, nasal swab, joint swab, tracheal wash | ||
| Avian influenza virus - AIV | Avian | H5/H7 PCR | $10.00 | $9.00 | M-F | same day | cloacal swab, oropharyngeal swab, tissue samples | ||
| Bovine enteric panel - BEP | Bovine, Caprine, Ovine | PCR | $50.00 | $45.00 | M-F | same day | feces, intestinal content, fecal swab, intestine |
Includes Rotavirus group A, BCV, Salmonella spp, E coli K99, Cryptosporidium parvum
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| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | PCR – Screening Type 1 (EU) and Type 2 (NA) | $25.00 | $22.50 | M-F | same day | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum | ||
| Porcine deltacoronavirus -PDCoV | Porcine | PCR - Singleplex | $25.00 | $22.50 | M-F | same day | feces, fecal swabs, intestine, oral fluids, environmental samples | ||
| Porcine reproductive & respiratory syndrome virus - PRRSV | Porcine | PCR - BIVI Ingelvac MLV-like | $35.00 | $31.50 | M-F | Same day | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
This assay tests for the presence of vaccine virus in the samples but it is not a differential assay and does not exclude a potential co-infection with a wild-type PRRSV.
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| Mycoplasma gallisepticum - AvMG | Avian | PCR | $30.00 | $27.00 | M-F | same day | oropharyngeal, and tracheal swabs | ||
| Influenza D Virus – IDV | Bovine, Porcine | PCR | $30.00 | $27.00 | M-F | next day | Lung, nasal swab, tracheal wash, pharyngeal swab | ||
| Mycoplasma hyopneumoniae - MHP | Porcine | ELISA | $5.50 | $4.95 | M-F | same day | serum, plasma | ||
| Porcine epidemic diarrhea virus - PEDV | Porcine | PCR – Prototype vs. Variant/INDEL | $30.00 | $27.00 | M-F | same day | feces, fecal swabs, intestine, oral fluids, environmental samples | ||
| Rabies | All Species | FA | $30.00 | M-F | 1-2 business days | brain | |||
| Fungal | All Species | Culture | $25.00 | M-F | 2-3 weeks |
$25.00 1st sample; $10.00 each additional sample
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| Salmonella | All Species | PCR | $30.00 | $27.00 | M-F | Next-day | Feces, intestines, culture | ||
| Bovine respiratory viral and bacterial panel - BRP | Bovine, Caprine, Ovine | PCR | $80.00 | $72.00 | M-F | same day | lung, nasal swab, pharyngeal swab, tracheal wash, BAL |
Includes Bovine Coronavirus, Infectious Bovine Rhinotracheitis, Bovine Respiratory Syncytial Virus, Bovine Viral Diarrhea Virus, Mycoplasma bovis, Mannheimia haemolytica, histophilus somni, Pasteurella multocida
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| Mycoplasma hyopneumoniae - MHP | Porcine | PCR | $30.00 | $27.00 | M-F | same day | lung, bronchial swab, BAL, oral fluids, nasal swab, laryngeal swab, tracheal swab | ||
| Porcine respiratory coronavirus – PRCV | Porcine | PCR | $35.00 | $31.50 | M-F | same day | lung, nasal swab | ||
| Porcine reproductive & respiratory syndrome virus - PRRSV^ | Porcine | Elanco Prevacent MLV CLAMP Sequencing (ORF5 region, ~600bp) | $220.00 | $198.00 | M-F | 2-4 days | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
Sample amount: no less than 1 mL fluid, 3 grams of tissue. Success rate: PRRSV screening PCR with a a ct of 30 or lower is recommended. Cost: ORF5 CLAMP PCR - $90.00 Sequencing and analysis - $65 per sequence recovered, maximum of 2. Total maximum cost=$220.
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| Porcine parainfluenza virus type 1 – PPIV-1 | Porcine | PCR | $30.00 | $27.00 | M-F | same day | lung, nasal swabs, bronchial swab, BAL, oral fluids | ||
| Bovine respiratory viral panel - BRP Viral | Bovine, Caprine, Ovine | PCR | $40.00 | $36.00 | M-F | same day | lung, nasopharyngeal swab, TTW, BAL |
Includes Bovine Coronavirus, Infectious Bovine Rhinotracheitis, Bovine Respiratory Syncytial Virus, and Bovine Viral Diarrhea Virus
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| Mycoplasma hyopneumoniae - MHP | Porcine | PCR - Quantitative | $32.00 | $28.80 | M-F | same day | lung, bronchial swab, BAL, oral fluids, nasal swab, laryngeal swab, tracheal swab | ||
| Salmonella | Bovine, Canine, Caprine, Equine, Feline, Ovine, Porcine | Broth Enrichment | $10.00 | M-F | 2-3 days |
This fee is in addition to the routine culture fee ($15.00 - 25.00 depending on sample type).
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| Bovine respiratory bacterial panel - BRP Bacti | Bovine, Caprine, Ovine | PCR | $40.00 | $36.00 | M-F | same day | lung, pharyngeal swab, tracheal wash, BAL |
Includes Mycoplasma bovis, Mannheimia haemolytica, Histophilus somni, Pasteurella multocida
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| Clostridioides difficile | All Species | Culture | $15.00 | M-F | 1 week | ||||
| Salmonella | Bovine, Canine, Caprine, Equine, Feline, Ovine, Porcine | Bacterial serogrouping | $20.00 | M-F | 1-2 days |
This fee is added whenever a Salmonella sp. is isolated.
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| Porcine reproductive & respiratory syndrome virus- PRRSV | Porcine | PCR - Quantitative | $27.00 | $24.30 | M-F | same day | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum | ||
| Infectious bovine rhinotracheitis virus - IBR | Bovine | PCR | $30.00 | $27.00 | M-F | next day | lung, nasal swab | ||
| Clostridioides difficile | All Species | ELISA - toxin A/B | $30.00 | M-F | same day | colon, feces |
if received by 2pm
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| Streptococcus suis (S. suis) | Porcine | Bacterial serotyping | $20.00 | M-F | 1-2 days |
Turn around time: 2-4 days if isolation required
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| Porcine reproductive & respiratory syndrome virus- PRRSV^ | Porcine | Sequencing (ORF5 region, ~600bp) | $110.00 | $99.00 | M-F | 2-4 days | Blood swab, BAL, environmental, lung, oral fluid, processing fluid, semen, serum |
Sample amount: no less than 1mL fluid or 3 grams of tissue.
Success rate: PRRSV screening PCR with a ct of 35 or lower for other specimens is recommended.
Cost: ORF5 PCR -$45.00
Sequencing and analysis- $65.00
Total cost=$110.00.
^A portion of the sequencing test is performed at the DNA facility which is not ISO/AAVLD accredited, but sequences are analyzed at the VDL.
European PRRSV PCR is tested Tuesday and Friday with a 2-4 day turn-around time.
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| Bovine respiratory syncytial virus - BRSV | Bovine, Caprine, Ovine | PCR | $30.00 | $27.00 | M-F | same day | lung, nasal swab, pharyngeal swab, tracheal wash, BAL | ||
| Porcine circovirus type 3 – PCV3 | Porcine | PCR - Quantitative | $32.00 | $28.80 | M-F | same day | lung, lung swab, serum, blood swab, processing fluid, oral fluid | ||
| Bovine viral diarrhea virus - BVDV | Bovine | IHC (4 or more notches) | $3.75 | $3.38 | M-F | next day | formalin-fixed ear notches |
$3.75 per notch. For surveillance cases only.
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| Parasitology | All Species | Baermann | $25.00 | M-Th | next business day | feces (5 grams) |
This assay requires at least 5 grams of feces and is run overnight and reported the next day.
If submitting greater than 10 fecal samples, please contact the laboratory for scheduling and approximate turnaround time.
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| Avian influenza virus - AIV | Avian | AGID - serum | $3.00 | $2.70 | M-Th | 2-day test | serum | ||
| West nile virus - WNV | Equine | IgM ELISA | $45.00 | $40.50 | M-Th | next day | serum | ||
| Avian influenza virus - AIV | Avian | AGID - egg yolk | $3.50 | $3.15 | M-W | 2-day test | egg/yolk | ||
| Brucella | Bovine, Equine, Ovine, Porcine | Bruc. STT | $2.50 | $2.25 | M-W | 3-day test | serum | ||
| Porcine epidemic diarrhea virus - PEDV | Porcine | PCR - Quantitative | $27.00 | $24.30 | same day | M-F | feces, fecal swabs, intestine, oral fluids, environmental samples | ||
| Salmonella | Porcine | ELISA | $8.00 | $7.20 | T | Same day | Serum, plasma, meatjuice | ||
| Anaplasma marginale - Ana | Bovine | cELISA | $6.00 | $5.40 | T, Th | same day | serum | ||
| Leptospira | Bovine, Camelid, Canine, Caprine, Equine, Ovine, Porcine, Ungulates | MAT 6 serovars | $6.00 | $5.40 | T, Th | same day | serum, fetal thoracic fluid |
6 serovars = canicola, ictero, grippo, pomona, hardjo, bratislava (Bison)
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| Influenza A virus - IAV | Porcine | VI | $50.00 | T, Th | 7-21 days | lung, nasal swab, BAL, oral fluids | |||
| Leptospira | Bovine, Camelid, Canine, Caprine, Equine, Ovine, Porcine, Ungulates | MAT 5 serovars | $5.50 | $4.95 | T, Th | same day | serum, fetal thoracic fluid |
5 serovars = canicola, ictero, grippo, pomona, hardjo
(Bison)
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| Bovine leukemia virus - BLV | Bovine, Ungulates | ELISA | $5.00 | $4.50 | T, Th | same day | serum |
Bison
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| Porcine epidemic diarrhea virus - PEDV | Porcine | S1 FMIA Luminex | $9.00 | $8.10 | T, Th | same day | Serum, colostrum, milk | ||
| Avian metapneumovirus - aMPV | Avian | ELISA | $2.50 | $2.25 | Th | same day | serum | ||
| Transmissible gastroenteritis virus / Porcine respiratory coronavirus - TGEV/PRCoV | Porcine | ELISA differential | $9.50 | $8.55 | Th | same day | serum | ||
| Hormone-free panel - feed | All Species | $150.00 | Th | 3 business days | feed (200g) Corn, DDGS, Feed, Grain, Mixed Feed, Silage, Other, Total Mixed Ration |
STAT fee $300 for 1st sample. $150 for each additional sample.
Panel includes: Clenbuterol, MGA (melengestrol acetate), Ractopamine, and Zilpaterol. Feed Guidelines link
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| Pasteurella multocida | Porcine | PCR - Dermonecrotic toxin | $30.00 | $27.00 | Th | next day | culture | ||
| Porcine circovirus type 2 - PCV2 | Porcine | IFA endpoint | $15.00 | Th | same day | serum | |||
| Ornithobacterium rhinotracheale - ORT | Avian | ELISA | $3.00 | $2.70 | Th | same day | serum |
Chicken or turkey serum
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| Porcine circovirus type 2 - PCV2 | Porcine | IFA 4 dilution | $7.50 | Th | same day | serum | |||
| Chronic wasting disease - CWD | Ungulates | ELISA | $25.00 | Th | same day | FRESH tissues:Medial retropharyngeal lymph node ANDobex |
$15.00 - farm raised; $25.00 - free roaming; $12.50 - preserve
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| Clostridium perfringens | All Species | PCR - toxin genotyping (alpha, beta, epsilon, iota, enterotoxin, beta2) | $30.00 | $27.00 | Th | next day | culture | ||
| Antibiotic Panel - Feed | All Species | LC-MS | $150.00 | Tu | 3 business days | Corn, DDGS, Feed, Grain, Mixed Feed, Silage, Other, Total Mixed Ration |
STAT fee is $300 for first sample, $150 for each additional.
Panel includes: Bacitracin Methylene Disalicylate, Bacitracin Zinc, Carbadox, Chlortetracycline, Florfenicol, Lincomycin, Naracin, Oxytetracycline, Penicillin, Sulfamethazine, Sulfathiazole, Tiamulin, Tilmicosin, Tylosin, Tylvalosin, Virginiamycin
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| Porcine circovirus type 2 - PCV2 | Porcine | ELISA | $6.00 | $5.40 | Tu | same day | serum | ||
| Mycoplasma hyopneumoniae - MHP | Porcine | CIVTEST Suis MHP ELISA | $10.00 | $9.00 | Tu, F | same day | serum | ||
| Actinobacillus pleuropneumoniae - APP | Porcine | ApxIV ELISA | $6.00 | $5.40 | W | same day | Serum, plasma |
Detects antibody against the ApxIV toxin produced by some Actinobacillus pleuropneumoniae. DOES NOT DETECT SEROTYPE. Useful for population surveillance.
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| Actinobacillus pleuropneumoniae - APP | Porcine | CF 1, 5, 7 (no serotyping) | $5.00 | $4.50 | W | 2-day test | serum | ||
| Actinobacillus pleuropneumoniae - APP^^ | Porcine | ApxIV OF ELISA (R&D) | $9.00 | $8.20 | W | same day | oral fluids |
Detects antibody against the ApxIV toxin produced by some Actinobacillus pleuropneumoniae. DOES NOT DETECT SEROTYPE. Useful for population surveillance.
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| Avian reovirus - AvRV | Avian | ELISA | $2.50 | $2.25 | W | same day | serum | ||
| Actinobacillus pleuropneumoniae - APP | Porcine | CF Serotype 3 | $5.00 | $4.50 | W | 2-day test | serum | ||
| Ionophore Panel | All Species | $100.00 | W | next day | feed (200g) |
Panel includes: Lasalocid, Monensin, Narasin, and Salinomycin.
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| Lasalocid | All Species | W | next day | feed |
See Ionophore Panel
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| Lawsonia intracellularis | Porcine | ELISA blocking | $8.00 | $7.20 | W | same day | serum, plasma | ||
| Infectious bursal disease - IBD | Avian | ELISA | $2.50 | $2.25 | W | same day | serum |
validated for chicken only
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| Individual Antibiotic Quantitation - Feed | All Species | quantitation by LC-MS | $150.00 | W | 3 days | feed (preferred), feedstuff, fecal, other |
Stat charge is $300.00
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| Monensin | All Species | W | next day | feed |
See Ionophore Panel
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| Mycoplasma hyorhinis - MHR | Porcine | IgG ELISA | $8.00 | $7.20 | W | same day | serum | ||
| Newcastle disease virus - NDV | Avian | ELISA | $2.00 | $1.80 | W | same day | serum | ||
| Mycoplasma hyosynoviae - MHS | Porcine | MHS T20 IgG ELISA | $8.00 | $7.20 | W | Same day | Serum | ||
| Avian encephalomyelitis virus - AEV | Avian | ELISA | $2.75 | $2.48 | W | same day | serum |
validated for chicken only
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| Infectious bronchitis virus - IBV | Avian | ELISA | $2.75 | $2.48 | W | same day | serum |
validated for chicken only
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| Bovine viral diarrhea virus-BVDV | Bovine | AgELISA | $6.00 | $5.40 | W, F | same day | serum, ear notches (fresh, not fixed) | ||
| Neospora caninum | Bovine | cELISA | $6.00 | $5.40 | W, F | same day | serum | ||
| Mycobacterium paratuberculosis (Johne's) | Bovine | ELISA | $5.00 | $4.50 | W, F | same day | serum, plasma, milk |
Please visit https://vetmed.iastate.edu/vdpam/about/FSVM/beef/bovine-disease-topics/johnes-disease for more information.
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| Pseudorabies - PRV | Porcine | ELISA gB (screen) | $3.50 | $3.15 | W, F | same day | serum, meat juice |