Sample Collection

Collecting and Submitting Oropharyngeal Swabs for AIV PCR

First Case of Highly Pathogenic Avian Influenza in Iowa reported - April 14, 2015

To properly obtain samples for AIV PCR you will need to have BHI broth tubes, dakron/polyester swabs, scissors, and gloves as well as 5 fresh mortalities. In addition you will need a cooler, cold pack and submission form for submitting samples to the lab.

Recommended Supplies:

BHI broth:


Sample Collection:

  • For properly attaining and pooling samples, please reference this step by step video demonstrating sample collection, pooling, and packaging.
  • The Center for Food Safely and Public Health also has this printable step by step guide.


  • Fill out laboratory form.
  • Include: Farm name, Premises ID, date and number of samples
  • Place form and labeled tubes in two separate sealable bags.
  • Place the bags in a cooler with cold pack.
  • Ship overnight or hand deliver to:
    Veterinary Diagnostic Laboratory
    Iowa State University
    1850 Christensen Dr
    Ames, IA 50011-1134