Tracheal / Oropharyngeal Swabs for AIV PCR

To properly obtain samples for AIV PCR you will need to have BHI broth tubes, polyester swabs, scissors, and gloves as well as fresh mortalities. In addition, you will need a cooler, cold pack, and submission form for submitting samples to the lab.

Recommended Supplies:

Gloves

Poultry shears

Sharpie 

ISU VDL Label

BHI broth (Hardy Diagnostics)

Polyester swabs (ThermoFisher Scientific, Hardy Diagnostics, Puritan)

Sample Collection:

1. Prepare supplies and put on gloves.

2. Open the bird at the commissure of the beak using the poultry shears.

3. Swab the trachea/oropharynx using a polyester swab. 

4. Swirl the swab in the BHI broth vigorously. Remove the swab and discard it. 

5. Collect up to 11 swabs in 1 BHI tube.

• DO NOT send dry swabs.
• DO NOT keep swabs in the tube.
• IF splitting samples - swab the same bird twice and use 2 separate BHI tubes. 

6. Place the BHI tube in a bag and label it with the ISU VDL Web ID, submitter name, and site. 

Submission Form:

1. Fill out the Avian Test Request Form via the ISU VDL Web Portal. 

• For more information on the ISU VDL Web Portal, see our Web-Based Tools page.
• If needed, a paper form is available here.

Shipping:

1. Place the bags (and paper form, if used) in a cooler with a cold pack.

2. Ship overnight or hand-deliver to:
 

Veterinary Diagnostic Laboratory

Iowa State University

1850 Christensen Drive

Ames, Iowa 50011