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Pathology

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Faculty and Staff

 
  • Dr. Darin Madson, Section Leader
  • Dr. Paulo Arruda
  • Dr. Eric Burrough
  • Dr. V. L. Cooper
  • Dr. Rachel Derscheid
  • Dr. Phil Gauger
  • Dr. Drew Magstadt
  • Dr. Tanja Opriessnig
  • Dr. Pablo Piñeyro
  • Dr. Kent Schwartz
  • Dr. Greg Stevenson
  • Dr. Darrell Trampel (poultry)
  • Ronisue Aust
  • Joe Brodie
  • Rosie Branaman
  • Dawn Fenneman 
  • Victoria Green
  • Michelle Grabosch
  • Scott Kostohryz
  • Franco Matias Ferreyra
  • Linda Smith
  • Jerry Snyder
  • Bonnie Snovelle
  • Jessica Heyer
  • Jessica Tuttle
  • Nicole Van Sickle
  • Aisha Azher
  • Kimberly Hunter
     

Dr. Pablo Piñeyro Joines VDL

Dr. Piñeyro obtained his DVM in 1999 from the Facultad de Ciencias Veterinarias, Universidad Nacional de la Plata in Buenos Aries, Argentina. He spent several years in private practice working with food animals in Argentina, and returned to the Universidad Nacional de la Plata where he worked in the food animal diagnostic laboratory and field investigation unit while completing a Doctorate in Veterinary Science (swine influenza). Dr. Piñeyro came to the U.S. and earned a Master of Veterinary Science degree (Vibrio parahaemolyticus) in 2010 while working in the VDL at Washington State University. He then went on to complete a Pathology Residency program and will soon earn a PhD degree (molecular virology and immunology of PCV and PRRSV) from Virginia Tech.

Dr. Piñeyro’s experience working with food animals in private practice, his extensive training in diagnostic pathology and molecular microbiology and immunology, and his passion for continuing his career in a swine focused veterinary diagnostic laboratory will make him a great addition to the ISU VDL team.

Pathology Submission Guidelines

Submission of tissue specimen(s) is often the best method of obtaining a diagnosis. However, proper selection and preservation of samples is essential to make the most efficient and economical use of the laboratory. The two conditions that most frequently interfere with diagnosis are (1) post mortem autolysis, and (2) sample collection too late in the course of disease.

Fresh specimens should be large enough to demonstrate the lesion yet small enough to allow for rapid chilling. In some cases (e.g., bronchoalveolarlavage for PRRS virus isolation) submission of an entire organ may be preferred. Ideally, fresh samples should be packaged individually to prevent cross-contamination. It is absolutely vital NOT to package fresh intestine with other tissues, as this results in fecal contamination of other organs. At a minimum, intestinal samples should be submitted separate from all other tissues. Samples should be shipped in leak-proof containers with artificial freeze packs. Please line shipping containers with additional sealed plastic bags to avoid leakage in transit. A fee of $25 may be assessed for packages that leak. Specimens for histopathology should include slices of the appropriate organs, including the lesion, transitional zones, and adjacent grossly normal tissue, in 10% buffered formalin in a leak-proof, wide-mouthed solid container. DO NOT USE GLASS CONTAINERS. When in doubt, collect specimens from multiple organs, including brain. The pathologist can then select the most appropriate specimens for complete microscopic examination. Unless it is important that individual animals be examined and reported separately, specimens from each individual animal can be pooled in a single container - provided the specimens are small enough to maintain a 10:1 formalin:tissue ratio. The ratio of formalin:tissue must be at least 10:1 to allow adequate fixation.

A minimum of 48 hours is required for histopathology, 24 hours for fixation and 24 hours for processing. Consequently, specimens for histopathological examination should be collected and placed in formalin at the time of necropsy in order to minimize autolysis and generally allow results to be available the day after the specimen is received.

General guidelines for histopathologic examination/submission of formalin-fixed specimens are as follows:

Solid organs: 0.5 - 1 cm slices to include lesion and transitional areas between lesions and normal tissue.

Intestine: 1 - 2 cm lengths held open as they are immersed in formalin or flushed with formalin prior to immersion. Do NOT tie the ends.
Brain (including brain stem): immerse sagittal half of small brain in formalin. One-half of the brain should also be submitted from larger animals. These larger brains should be sliced for better fixation. The preferred procedure is to make transverse slices, 1.0 - 1.5 cm apart that extend to within 1.0 cm of the ventrum of the brain. This allows for more rapid fixation while retaining the architecture of the brain so that specific areas can be sampled. Nerves can be pinned to a tongue depressor and immersed in formalin.

Tumors: immerse in 10% formalin. If greater than 1 cm, incise, leaving a base attachment intact.

Impression smears and needle aspirates of tumors are helpful in a few situations. However, actual biopsies are usually required for definitive diagnosis. Stain and examine smears and aspirates in your practice laboratory and, if a diagnosis is not obvious (i.e., mastocytoma, abscess, etc.), biopsy the mass and forward both the biopsy and the smears to the reference laboratory.

HANDY DOWNLOADS

New Quick Guide Factsheets

• Bovine Guidelines

• Ovine Guidelines

• Porcine Guidelines

 

 

QUICK LINKS

 

User Guide

Fee Schedule

Submission form

Parasitology

 


 

Contact info:

Veterinary Diagnostic Lab
1600 South 16th St 
Ames, IA  50011
515-294-1950

Fax: 515-294-3564

 

 

 

 

 

Be sure your submissions do not arrive at the lab
looking like this
Seal submission forms
in a plastic bag