ISU qPCR Consultation Service: contact eag@iastate.edu

ISU Consultation Service for qPCR Assay Development and Project Management. In person and on-line consultation for comprehensive qPCR theory and assay design assistance from beginning to end. All steps discussed and/or printed out as easy-to-follow recipes for immediate in-lab use. Get it right the first time.
Read typical recent reviews from ISU and abroad. Office of Biotechnology website announcement.

General Services offered:

Basic information/qPCR theory and math
Primer-probe design assistance (Example primer dilution help file as part of this service)
Identifying appropriate reagents, master mixes and machine platforms for One-
and Two-Step qPCR, including LCM-qPCR
MIQE-based RNA isolation, DNAse treatment and reverse transcription reaction
formulation suggestions and guidance
Nucleic acid quality assessment and quantity measurement suggestions and guidance
Processing of global assay parameters using ISURF software #03407
(Click here to view assorted screen shots of the program)
Consultation on detecting and avoiding RT and PCR inhibition for all sample types
and isolation methods
File system creation and initial qPCR Test Plate set-up printouts and consultation
Processing of Test Plate results into final set-up parameters and procedural printouts
for final sample qPCR
Excel spreadsheets custom-created for E_AMP-corrected data analysis and graphing
All plant and animal species considered
Note: due to the time-intensity and detailed nature of quality qPCR execution, urgent
projects (those which are requested to be carried out under very tight deadlines) will
carry heavier consultation charges than projects of 'normal' urgency