Dr. Miller’s research focuses on the interface between viruses and the cells they infect. Several projects in the laboratory focus on defining, at a molecular level, ways in which segmented double-stranded RNA viruses of the Reoviridae family commandeer cellular space and proteins to replicate their genomes, translate their proteins, and assemble into progeny virions. These projects focus on understanding the inner workings of distinct inclusion structures (termed viral factories) formed within the cellular cytoplasm during viral infection, and determining what drives the creation of these structures, and what role they play in regulation of viral RNA transcription, translation and replication during infection. Dr. Miller has also been a key player in the development of a novel protein-protein interaction technology utilizing the capacity of a single reovirus protein to form viral factory-like structures and recruit other proteins to these structures. A second project in the Miller laboratory is focused on utilizing this technology to identify and define new viral protein-protein interactions, and further, to screen for drugs or small molecules that can inhibit these interactions to identify potential anti-viral therapies. Dr. Miller’s laboratory is additionally involved in a number of projects examining the molecular mechanisms involved in influenza A virus pathogenicity and immunology in both swine and humans.
Graduate level course focused on the molecular mechanisms of viral growth and interactions between viruses and the infected host cell. Offered every other fall.
1993, B.A., Microbiology, University of Missouri-Columbia
2001, Ph.D., Molecular Microbiology and Immunology, University of Missouri-Columbia
2001-2005, Post-doc, Microbiology and Molecular Genetics, Harvard Medical School
Gupta, P. and Miller, C. L. 2014. HIF-1a downregulation and apoptosis in hypoxic prostate tumor cells infected with oncolytic Mammalian orthoreovirus. Oncotarget, 5(1.2), 561-574.
Buehler, J., Lager, K., Vincent, A., Miller, C., Thacker, E., and Jahnke, B. 2014. Issues encountered in development of an ELISA for use in detecting H5N1 exposure in swine. Journal of Veterinary Diagnostic Investigation, 26(2), In press.
Carroll, K., Hastings, C., and Miller, C.L., 2014. Amino-acids 78 and 79 of mammalian orthoreovirus protein µNS are necessary for stress granule localization, core protein λ2 interaction, and de novo virus replication. Virology, 448: 133-145.
Kappes, M., Miller, C. L., and Faaberg, K. 2013. Highly Divergent Strains of Porcine Reproductive And Respiratory Syndrome Virus Incorporate Multiple Isoforms of Nonstructural Protein 2 into Virions. Journal of Virology 87:24 13456-13465.
,J. Navi, D., Lorusso, A., Vincent, A., Lager, K., and Miller, C.L. 2013. Influenza A virus PB1-F2 protein expression is regulated in a strain-specific manner by sequences located downstream of the PB1-F2 initiation codon J. Virol. 87(19):10687-99.
Chen, Q., Madson,D., Miller, C. L., and Harris, H. 2012. Vaccine development for protecting swine against influenza virus. Animal Health Research Reviews, 13(2); 181–195.
Hines, N., Killian, M., Pedersen, J., Reising, M. Mosos, N., Mathieu-Benson, C., and Miller, C. L. 2012. An rRT-PCR Assay to Detect the Matrix Gene of a Broad Range of Avian Paramyxovirus Serotype-1. Avian Diseases, 56(2):387-395.2012
Hines, N., and Miller, C. L. 2012. Avian Paramyxovirus Serotype-1: A Review of Disease Distribution, Clinical Symptoms and Laboratory Diagnostics. Veterinary Medicine International, Volume 2012, Article ID 708216, 17 pages, doi:10.1155/2012/708216.
Miller, C. L. 2011. Stress Granules and Virus Replication. Future Virology, Nov 2011, Vol. 6, No. 11, Pages 1329-1338.
Qin, Q., Hastings, C., Carroll, K., and Miller, C. L. 2011. Mammalian Orthoreovirus escape from host translational shutoff correlates with stress granule disruption and is independent of eIF2α phosphorylation and PKR. Journal of Virology 85 (17): 8798-8810.
Firth, A. E., B. J. Blitvich, N. M. Wills, C. L. Miller, J. F. Atkins. 2010. Evidence for ribosomal frameshifting and a novel overlapping gene in the genomes of insect specific flaviviruses. Virology 30;399(1):153-66.
Li, G, Y. Feng,, S. Kariyawasam, K. A. Tivendale, Y. Wannemuehler, F. Zhou, C. L. Miller, C. M. Logue, and L. K. Nolan. 2010. AatA is a novel autotransporter and virulence factor of avian pathogenic Escherichia coli. Infection and Immunity, 78.3: 898-906.
Miller, C. L., Arnold, M., Broering, T., Hastings, C., and Nibert, M. 2010. Localization of mammalian orthoreovirus proteins to cytoplasmic factory-like structures via nonoverlapping regions of μNS. Journal of Virology, 84 (2): 867-882. Co-corresponding author.
Qin, Q., Hastings, C., and Miller, C. 2009. Mammalian orthoreovirus particles induce and are recruited to cellular stress granules. Journal of Virology, 83(21):11090-101.
Miller, C. L., M. M. Arnold, T. J. Broering, J. Kim, J. B. Dinoso, and M. L. Nibert. 2007. A virus-derived platform for easily visualizing protein-protein associations in cells. Mol. Cell. Proteomics 6: 1027 – 1038. Co-corresponding author. Cover illustration.
Carroll, K., and Miller, C. L. 2013. Investigation of mammalian orthoreovirus escape from host translational shutoff. The 32nd American Society for Virology Meeting, July 20-24, University Park, PA. Oral presentation.
Gupta, P., and Miller, C. L. 2013. Mammalian Orthoreovirus infection of hypoxic prostate tumor cells induces downregulation of HIF-1a and apoptotic tumor cell death. 7th Int'l Meeting on Replicating Oncolytic Virus Therapeutics. June 15-18, Quebec City, Quebec, Canada. Oral and poster presentations.
Gupta, P., Darling, R., and Miller, C. 2012. Mammalian orthoreovirus induces apoptosis in hypoxic prostate tumor cells and downregulates HIF-1a via RACK1-mediated proteasome degradation and translational inhibition. The 31st American Society for Virology Meeting, July 21-25, Madison, WI. Oral presentation.
Carroll, K. and Miller, C. 2012. Investigation of the role of mammalian orthoreovirus muNS protein in stress granule modulation. The 31st American Society for Virology Meeting, July 21-25, Madison, WI. Oral presentation.
Buehler, J., Navi, D., Vincent, A., Lorusso, A., Lager, K., and Miller, C. 2012. Elucidating the role of virus lineage and RNA sequence in translational regulation of influenza virus PB1-F2. The 31st American Society for Virology Meeting, July 21-25, Madison, WI. Poster presentation.
Gupta, P., and Miller, C. L. 2012. Mammalian Orthoreovirus downregulates HIF-1a in hypoxic prostate tumor cells via RACK1-mediated proteasomal degradation and translational inhibition. 2012 American Association for Cancer Research Annual Meeting. Mar 31-Apr 4, Chicago, IL. Poster Presentation. Selected as a Late Breaking Abstract.
Gupta, P. and Miller, C. 2011. Investigation of the impact of mammalian orthoreovirus infection on hypoxic cancer cells. American Society for Virology 30th Annual Meeting, University of Minnesota, Minneapolis, MN, July 16-20. Oral Presentation
Buehler, J., McCullough, D., Kohns, C., Loving, C., Lorusso, A. Vincent, A., Lager, K., and Miller, C. 2011. Characterization of swine influenza virus PB1-F2 properties in swine and human cells. American Society for Virology 30th Annual Meeting, University of Minnesota, Minneapolis, MN, July 16-20. Oral Presentation.
Qin, Q., Hastings, C., and Miller, C. 2010. Disruption of Stress Granules is Important for Mammalian Orthoreovirus Escape from Host Translational Shutoff. RNA in Motion Symposium, Sept. 9-12, Ames, IA. Poster presentation.
Miller, A. G., Gupta, P., and Miller, C. 2010. Investigation of the impact of mammalian orthoreovirus infection on hypoxic tumor cells. 10th Annual Merial-NIH National Veterinary Scholars Symposium. Athens, GA, August 5-8. Poster Presentation. Poster Award, 1 of 6 from 87 posters.
Cutler TD, Hoff SJ, Wang C, Warren KJ, Zhou F, Qin Q, Miller C, Ridpath JF, Yoon K-J, Zimmerman J. September 2010. Inactivación de agents patógenos virales seleccionados mediante luz ultravioleta (UV254). XXII Congreso Panamericano de Ciencias Veterinarias. Lima, Peru.
Qin Q., Gupta, P, Hastings, C., and Miller, C. L. 2010. Mammalian orthoreovirus translation is refractory to phosphorylation of eIF2α caused by oxidative stress or hypoxia. American Society for Virology 29th Annual Meeting, Montana State University, Bozeman, MT, July 17-21. Oral Presentation.
Buehler, J., McCullough, D., Loving, C., Vincent, A., Lager, K., and Miller, C. 2010. Investigation of the function of the influenza A virus PB1-F2 protein during infection of swine and human cells with a predominant circulating strain. American Society for Virology 29th Annual Meeting, Montana State University, Bozeman, MT, July 17-21. Poster Presentation.
Qin, Q., Hastings, C., and Miller, C. 2010. Disruption of Stress Granules is Important for Mammalian Orthoreovirus Escape from Host Translational Shutoff. Keystone Symposium on Cell Biology of Virus Entry, Replication, and Pathogenesis. Feb. 17-21, Taos, NM. Poster presentation.
Cutler T, Hoff S, Wang C, Warren K, Zhou F, Qin Q, Miller C, Ridpath J, Yoon K-J, Zimmerman J. October 2009. UV254 Inactivation of Selected Viral Pathogens. 52st Annual Conference, American Association of Veterinary Laboratory Diagnosticians. San Diego, California, p. 124. Oral presentation.
Cutler TD, Hoff SJ, Wang C, Warren KJ, Zhou F, Qin Q, Miller C, Ridpath JF, Yoon K-J, Zimmerman J. December 2009. UV254 inactivation of selected viral pathogens. Proc 2009 International PRRS Symposium. Chicago, Illinois, p 66. Poster presentation.
Buehler, J., Thacker, E., Lager, K., Miller, C., and Janke, B. Serologic detection of H5 Influenza virus infection in swine using ELISA techniques. The 5th International Veterinary Vaccines and Diagnostics Conference. July 19-23, Madison WI. Oral and Poster Presentation.
Qin, Q., Hastings, C., and Miller, C. L.. Mammalian orthoreovirus infection interferes with stress granule formation. American Society of Virology 27th Annual Meeting, July12-16, 2008, Ithaca, New York. Poster presentation.
Chair, Interdepartmental Microbiology Program
1995-1996 Donald K. Anderson Teaching Award University of Missouri-Columbia Medical School
1995-1999 Pre-doctoral Fellow, NIH, NIAID Institutional NRSA Training Grant Department of Molecular Microbiology and Immunology University of Missouri-Columbia School of Medicine
2001-2002 Ernst Postdoctoral Fellow Harvard Medical School
2002-2003 Postdoctoral Fellow, NIH, NIAID Institutional NRSA Training Grant Infectious Disease and Basic Microbiological Mechanisms Harvard Medical School
2003-2005 Postdoctoral Fellow, NIH, NIAID Individual NRSA Training Grant Harvard Medical School
2006-2008 Principal Investigator, NIH, NIAID Career Development Award Iowa State University
2008-2011 Principal Investigator, The Roy J. Carver Charitable Trust Medical and Scientific Research Grant
2008-2011 Principal Investigator, USDA Specific Cooperative Agreement 58-3625-8-712
2009-2011 Principal Investigator, Iowa Pork Board Research Grant
2010-2013 Principal Investigator, NIH NIAID R15AI090635 Research Grant
2010-2013 Principal Investigator, USDA Specific Cooperative Agreement 58-3625-0-650
2011-2014 Principal Investigator, Margaret B. Barry Cancer Research Award
2001-Present American Society for Virology, Associate Member
2005-Present American Association for the Advancement of Science
2008-Present American Society for Microbiology