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F. Chris Minion

Chris Minion
Chris Minion

M.S., Ph.D.

Professor
Vet Microbiology & Preventive Medicine
(515) 294-6347
1130 Vet Med
Expertise / Research Area

Dr. Minion's research focuses on molecular mechanisms of microbial pathogenesis, pathogenomics, proteomics, gene expression profiling and molecular vaccines. Current projects include the role of biofilms of E. coli O157:H7 in persistence in ruminants; gene expression profiling of microbial pathogens, molecular pathogenesis of Mycoplasma hyopneumoniae; and the development of novel vaccines against Yersinia pestis.

Teaching

MICRO 552 Bacterial Molecular Genetics and Physiology. (1-0) Cr. 1. F. Prereq: 302, Biol 313. Review of the molecular genetics and physiology of model organisms.

V MPM 625. Mechanisms of Bacterial Pathogenesis. (4-0) Cr. 4. Alt. S., offered 2013. Prereq: Credit in Biochemistry and Microbiology. Review of current concepts in specific areas of microbial pathogenesis including the genetic basis for bacterial disease, genetic regulation and control of virulence factors and their mechanisms of action, and host-pathogen interactions at the cellular and molecular levels. The application of microbial genetics to understanding pathogenesis.

Education

University of Alabama in Birmingham - Ph.D. in Molecular and Cellular Biology, 1983, Department of Microbiology

University of Memphis - M.S. in Cell Biology, 1977

University of Memphis - B.S. in Chemistry, 1972

Selected Publications

Minion, F. C., E. L. Lefkowitz, M. L. Madsen, B J. Cleary, S. M. Swartzell, and G. G. Mahairas. 2004. The genome sequence of Mycoplasma hyopneumoniae strain 232, the agent of swine mycoplasmosis. J. Bacteriol. 186:7123-7133.

Madsen, M. L., D. Nettleton, E. L. Thacker, R. Edwards, and F. C. Minion. 2006. Transcriptional profiling of Mycoplasma hyopneumoniae during heat shock using microarrays. Infect. Immun. 74: 160-166.

Jenkins, C., J. L. Wilton, F. C. Minion, L. Falconer, M. J. Walker, and S. P. Djordjevic. 2006. Two domains within the Mycoplasma hyopneumoniae cilium adhesin bind heparin. Infect. Immun. 74:481-487.

Burnett, T.A., K. Dinkla, M. Rohde, G.S. Chhatwal, M. Srivastava, S.J. Cordwell, S. Geary, F. C. Minion, M. J. Walker, S. P. Djordjevic. 2006. P159 is a proteolytically processed, surface adhesin of Mycoplasma hyopneumoniae: defined domains of P159 bind heparin and promote adherence to eukaryotic cells. Mol. Microbiol. 60:669-686.

Madsen, M. L., D. Nettleton, E. L. Thacker, and F. C. Minion. 2006. Transcriptional profiling of Mycoplasma hyopneumoniae during iron depletion using microarrays. Microbiology 152:937-944.

Schafer, E. R., M. J. Oneal, M. L. Madsen, F. C. Minion. 2007. Global transcriptional analysis of Mycoplasma hyopneumoniae following exposure to hydrogen peroxide. Microbiol. 153:3785-3790.

Madsen, M. L., M. J. Oneal, S. W. Gardner, E. L. Strait, D. Nettleton, E. L. Thacker, and F. C. Minion. 2007. Array-based genomic comparative hybridization of field strains of Mycoplasma hyopneumoniae. J. Bacteriol. 189:7977-7982.

Madsen, M. L., S. Puttamreddy, E. L. Thacker, M. D. Carruthers, F. C. Minion. 2008. Transcriptome changes in Mycoplasma hyopneumoniae during infection. Infect. Immun. 76:658-663.

Chang, L.-J., W.-H. Chen, F. C. Minion and D. Shiuan. 2008. Mycoplasmas regulate the expression of heat shock protein genes through CIRCE-HrcA interactions. Biochem. Biophys. Res. Commun. 367:213-218.

Puttamreddy, S., M. D. Carruthers, M;. L. Madsen, and F. C. Minion. 2008. Transcriptome analysis of organisms with food safety relevance. Foodborne Pathog. Dis. 5:517-529.

Oneal, M. J., E. R. Schafer, M. L. Madsen and F. C. Minion. 2008. Global transcriptional analysis of Mycoplasma hyopneumoniae following exposure to norepinephrine. Microbiol. 154:2581-2588.

Wilton, J., C. Jenkins, S. Cordell, L. Falconer, F. Minion, D. Oneal, M. Djordjevic, A. Connolly, I. Barchia, M. Walker, and S. P. Djordjevic. 2009. Mhp493 (P216) is a proteolytically processed, cilium and heparin binding protein of Mycoplasma hyopneumoniae. Mol. Micro. 71: 566-582.

Carruthers, M. D., and F. C. Minion. 2009. Transcriptome analysis of Escherichia coli O157:H7 during heat shock. FEMS Microbiol. Lett. 295:96-102.

Carrillo-Conde, E. Schilitz, J. Yu, F. C. Minion, G. J. Phillips, M. Wannemuehler, B. Narasimhan. 2010. Encapsulation into amphiphilic polyanhydride microparticles stabilizes Yersinia pestis antigens. Acta Biomaterialia 6:3110-3119.

Puttamreddy, S., N. A. Cornick, and F. C. Minion. 2010. Genome-wide transposon mutagenesis of Escherichia coli O157 EDL933 identifies pO157 genes involved in biofilm formation. Infect. Immun. 78:2377-2384.

Gardner, S. W. and F. C. Minion. 2010. Detection and quantification of intergenic transcription in Mycoplasma hyopneumoniae. Microbiol. 156: 2305-2315.

Carruthers, M. D., B. H. Bellaire, and F. C. Minion. 2010. Exploring the response of Escherichia coli O157:H7 EDL933 within Acanthamoeba castellanii by genome-wide transcriptional profiling. FEMS Microbiol. Lett. 312: 15-23.

Deutscher, A. T., C. Jenkins, F. C. Minion, M. Padula, L. M. Seymour, N. E. Dixon, M. J. Walker, and S. P. Djordjevic. 2010. Repeat regions R1 and R2 in the P97 paralog Mhp271 of Mycoplasma hyopneumoniae bind heparin, fibronectin, and porcine cilia. Mol. Micro. 78:444-458.

Seymour, L. M., A. T. Deutscher, C. Jenkins, T. A. Kuit, L. Falconer, F. C. Minion , B. Crossett, M. Padula, N. E. Dixon, S. P. Djordjevic, M. J. Walker. 2010. A processed multidomain Mycoplasma hyopneumoniae adhesin binds fibronectin, plasminogen and swine respiratory cilia. J. Biol. Chem.  285:33971-33978.

Puttamreddy, S., and F. C. Minion. 2011. Linkage between cellular adherence and biofilm formation in Escherichia coli O157:H7 EDL933. FEMS Microbiol. Lett. 315:46-53.

Seymour, L. M., L. Falconer, A. T. Deutscher, F. C. Minion, M. P. Padula, N. E. Dixon, S. P. Djordjevic and M. J. Walker. 2011. Mhp107 is a member of the
 multifunctional adhesin 
family of Mycoplasma hyopneumoniae. J. Biol. Chem. 286:10097-10104.

Kong, C. S., J. Yu, F. C. Minion, and K. Rajan. 2011. Identification of biologically significant genes from combinatorial microarray data. ACS Combi. Sci. 13:562-571.

Bogema, D. R, N. E. Scott, M. Padula, J. L. Tacchi, C. Jenkins, S. J. Cordwell, F. C. Minion, M. J. Walker, S. P. Djordjevic. 2012. The peptide sequence TTKF | QE defines the site of proteolytic cleavage in Mhp683 (P135), a novel glycosaminoglycan and cilium binding adhesin of Mycoplasma hyopneumoniae. J. Biol. Chem. 286:41217-41229.

Deutscher, A. T., J. L. Tacchi, F. C. Minion, M. P. Padual, B. Crossett, D. R. Bogema, C. Jenkins, M. J. Walker, S. P. Djordjevic. 2012. Mycoplasma hyopneumoniae surface proteins Mhp385 and Mhp384 bind host cilia and glycosaminoglycans and are endoproteolytically processed by proteases that recognize different cleavage motifs. J. Proteome Res. 11:1924-1936.

Bogema, D. R., A. T. Deutscher, L. M. Seymour, B. B. A. Raymond, J. L. Tacchi, M. P. Padula, N. E. Dixon, F. C. Minion, C. Jenkins, M. J. Walker, and S. P. Djordjevic. 2012. Characterization of cleavage events in the multifunctional cilium adhesin Mhp684 (P146) reveals a novel mechanism by which Mycoplasma hyopneumoniae regulates surface topography. mBio 3:e00282-11.

 Li, J., F. C. Minion, A. C. Petersen, F. Jiang, S. Yang, P. Guo, J. Li, and W. Wu. 2012. Loop-mediated isothermal amplification for rapid and convenient detection of Mycoplasma hyopneumoniae. World J. Microbiol. Biotechnol. DOI 10.1007/s11274-012-1216-x.

LaRock, C., J. Yu, A. R. Horswill, M. R. Parsek, and F. C. Minion. 2013. Transcriptome analysis of acyl-homoserine lactone-based quorum sensing regulation in Yersinia pestis. PLos One http://dx.plos.org/10.1371/journal.pone.0062337.

Yu, J., M. L. Madsen, M. D. Carruthers, G. J. Phillips, J. S. Kavanaugh, J. M. Boyd, A. R. Horswill, and F. C. Minion. 2013. Analysis of autoinducer-2 quorum sensing in Yersinia pestis. Infect. Immun. 81: 4053-4062.

Raymond, B. B. A., J. L. Tacchi, V. M. Jarocki, F. C. Minion, M. P. Padula, S. P. Djordjevic. 2013. P159 from Mycoplasma hyopneumoniae binds porcine cilia and heparin and is cleaved by multiple proteases in a manner akin to ectodomain shedding. J. Proteome Research 12: 5891-5903.

Leadership

University: Chair, University Biohazards and Public Health Committee (1999-2006). President, Iowa State University Graduate Council (2001-2003). Editorial Boards:  Infection and Immunity (2001-present); Journal of Clinical Microbiology (2012-present); Foodborne Pathogens and Disease (2004-present). ISU Faculty Senate (2009-present): CVM Faculty Senate Caucus Chair (2012-14); ISU Faculty Senate Resource Policies and Allocations Council (2009-present), ISU Faculty Senate Research Planning and Policy Committee (2011-present), Faculty Senate Executive Board (2012-present); Faculty Senate Representive Council (2012-present).

International: Information Officer, International Organization for Mycoplasmology and International Research Programme for Comparative Mycoplasmology (2002-present). Chair, International Research Programme for Comparative Mycoplasmology (1996-2000). Councilor at Large, American Society for Microbiology (1998-2000). Chair, Division G, American Society for Microbiology (1996-1997). Chair-elect, Division G, American Society for Microbiology (2012-13).

Honors & Awards

NIH Research Career Development Award (1991-1996), Louis Dienes Award in Mycoplasmology (1992), SmithKline Beecham Award for Research Excellence (1993)

Memberships

American Society for Microbiology

International Organization for Mycoplasmology

American Association for the Advancement of Science

Phi Zeta

Sigma Xi