Lisa K Nolan

Dr. Stephen G. Juelsgaard Dean of Veterinary Medicine
2506 Vet Med
Education & Certifications  

D.V.M.: University of Georgia, Athens, GA, 1988.
M.S.: Medical Microbiology, University of Georgia, Athens, GA, 1989.
Ph.D.: Medical Microbiology, University of Georgia, Athens, GA, 1992.

Research Focus & Interests  

Our research focuses on bacterial diseases of production animals, including their effects on animal health, human health and food safety.  The long-term goal of one of our projects is to understand the molecular pathogenesis of Extraintestinal Pathogenic Escherichia coli (ExPEC)-caused diseases.  ExPEC are the causative agents of such diverse diseases as avian colibacillosis and human urinary tract infection, neonatal meningitis, and sepsis.  Understanding ExPEC virulence, resistance, and host specificity should lead to more rational approaches for future disease control.  Also, we are exploring the role that plasmids play in the pathogenesis of bacterial disease and in the evolution of bacterial virulence and resistance.  Various approaches are used in these studies including classical bacteriologic, functional genomic, and proteomic techniques in order to gain an appreciation for the total host: pathogen interaction.

Honors & Awards  


  • Preferred AG Instructor, College of Agriculture Student Activities Council, NDSU, 1995
  • Mortar Board Preferred Professor, NDSU, 1996, 1997, 1998, 1999
  • Commendation, College of Agriculture Excellence in Teaching, Early Career, NDSU, 1996
  • Member, Phi Kappa Phi, 1998-
  • Mary McCannel Gunkelman 10th Annual Recognition Award, NDSU, 1998
  • University-Wide Odney Teaching Award, NDSU, 1999
  • Outstanding Academic Advisor, College of Agriculture, Mortar Board
  • National Honor Society, NDSU, 1999
  • Professorship, Walter F. and Verna Gehrts Presidential Professor, NDSU, 2001-2003
  • Distinguished Educator of the Year, Blue Key National Honor Society, NDSU, 2001


  • Associate Dean, Research and Graduate Studies, Iowa State University, Ames, Iowa, 2009-
  • Associate Dean, Academic and Student Affairs, Iowa State University, Ames, Iowa, 2007-2009
  • Executive Chair, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 2006-2007
  • Chair, Department of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, Iowa, 2003-2008
  • Interim Chair, Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, 2004-2005.
  • Director, Great Plains Institute of Food Safety, North Dakota State, 1999-2003
  • Member, North Dakota EPSCoR Steering Committee, 2001-2002
  • Member, Center for Advanced Technology Transfer and Traineeships Advisory Board, NDSU, 2002-2003
  • Member, NDSU Research Foundation Advisory Board, 2002-2003
  • Member, FDA-CVM's Veterinary Medicine Advisory Committee, 2003-2006
  • Member, Editorial Review Board, Avian Diseases, 2004-
  • Associate Editor, Diseases of Poultry, 12th edition, 2005-
  • American Veterinary Medical Association, 1984-
  • American Association of Avian Pathologists, 1991-
  • American Society for Microbiology, 1992-
  • International Society of Plasmid Biology, 2004-


Lab website:


Selected Publications  
Full publication list on PubMed
*Rodriguez-Siek, K.E., Giddings, C.W., Johnson, T.J., Fakhr, M., Doetkott, C., and Nolan, L.K. 2005. Comparison of Escherichia coli Implicated in Human Urinary Tract Infection and Avian Colibacillosis. Microbiol. 151: 2097-2110.
*Johnson, T.J., Siek, K.E., Johnson, S.J., and Nolan, L.K. 2005. DNA Sequence and Comparative Genomics of pAPEC-O2-R, an Avian Pathogenic Escherichia coli (APEC) Transmissible R Plasmid.  Antimicrob Agents Chemother. 49: 4681-4688. 
*Johnson, T.J., Siek, K.E., Johnson, S., and Nolan, L.K. 2006. Complete Sequence of a ColV Plasmid and Prevalence of Selected Plasmid-Encoded Virulence Genes among Avian Escherichia coli Strains. J Bacteriol. 188: 745-758
*Kariyawasam, S., Johnson, T.J., and Nolan, L.K. 2006. pap Operon of Avian Pathogenic Escherichia coli Strain O1 is Located on a Novel Pathogenicity Island. Infect Immun. 74: 744-749.
*Skyberg, J.A., Logue, C.M., and Nolan, L.K. 2006. Virulence Genotyping of Salmonella spp. with Multiplex PCR. Avian Dis. 50: 77-81.
Q. Li, J.A. Skyberg, M.K. Fakhr, J.S. Sherwood, L.K. Nolan, and C.M. Logue. 2006. Antimicrobial Susceptibility and Characterization of Salmonella Isolated from Processed Bison Carcasses. Appl Environ Microbiol. 72: 3046-3049.
*Lynne, A.M., Foley, S.L., and Nolan, L.K. 2006. Immune Response to Recombinant Escherichia coli Iss in Poultry. Avian Dis. 50: 273-276.
*Skyberg, J.A., Siek, K.E., Doetkott, C., and Nolan, L.K. 2006. Biofilm formation on Plastic Surfaces by Escherichia coli Isolated from Sick and Healthy Poultry. J Appl Microbiol. 102: 548-554.
*Lynne, A. M., Foley, S.L., and Nolan, L.K. 2006. Characterization of Monoclonal Antibodies to Avian Escherichia coli Iss. Avian Dis. 50: 445-449.
*Johnson, T.J., Johnson, S.J., and Nolan, L.K. 2006. Complete DNA Sequence of a ColBM Plasmid Occurring in an Extraintestinal Pathogenic Escherichia coli Isolate and Its Prevalence among Other E. coli Isolates. J Bacteriol. 188: 5975-5983.
*Kariyawasam, S., Johnson, T.J., DebRoy, C., and Nolan, L.K. 2006. Occurrence of PathogenicityIsland APEC-O1 Genes Among Escherichia coli Implicated in Avian Colibacillosis. Avian Dis. 50: 405-410.
*Skyberg, J.A., Johnson, T.J., Johnson, J.R., Clabots, C., Logue, C.M., and Nolan, L.K. 2006. Acquisition of Avian Pathogenic Escherichia coli Plasmids by a Commensal E. coli Isolate Enhances Its Abilities to Kill Chick Embryos, Grow in Human Urine, and Colonize the Murine Kidney. Infect Immun.  74: 6287-6292.
*Kariyawasam, S., Johnson, T.J., Siek, K.E., and Nolan, L.K. 2006. Unique DNA Sequences of Avian Pathogenic Escherichia coli Isolates as Determined by Genomic Suppression Subtractive Hybridization. FEMS Microbiol Lett. 262: 193-200.
*Lynne, A.M., Skyberg, J.A., Logue, C.M., and Nolan, L.K. 2007 (Online 2006). Detection of Iss and Bor on the Surface of Escherichia coliJ Appl Microbiol. 102: 660-666.
*Johnson, T.J., Wannemuehler, Y., Scaccianoce, J., Johnson, S.J., and Nolan, L.K. 2006. Complete DNA Sequence of an Avian Pathogenic Escherichia coli IncHI2 Plasmid Reveals Striking Similarities to Serratia marcescens Plasmid R478. Antimicrob Agents Chemother. 50: 3929-3933.
*Johnson, T.J., Kariyawasam, S., Wannemuehler, Y., Mangiamele, P., Johnson, S.J., Doetkott, C., Skyberg, J.A., Lynne, A.M., and Nolan, L.K. 2007. Genome Sequence of Avian Pathogenic Escherichia coli Strain O1:K1:H7 Shares Strong Similarities with Human ExPEC GenomesJ Bacteriol. 189: 3228-3236.
*Johnson, T.J., Wannemuehler, Y.M., Johnson, S.J., Logue, C.M., White, D.G., Doetkott, C., and Nolan, L.K. 2007. Plasmid Replicon Typing of Commensal and Pathogenic Types of Escherichia coliAppl Environ Microbiol. 73:1976-1983.
*Lynne, A.M., Skyberg, J.A., Logue, C.M., and Nolan, L.K. 2007. Characterization of a Series of Transconjugant Mutants of an Avian Pathogenic Escherichia coli Isolate for Resistance to Serum Complement. Avian Dis. 51:771-776.
Trampel, D.W., Wannemuehler, Y., and Nolan, L.K. 2007. Characterization of APEC from Peritonitis Lesions in Commercial Laying Hens. Avian Dis. 51:840-4.
*Kariyawasam, S., Scaccianoce, J., and Nolan, L.K. 2007. Common and Specific Virulence-Associated Markers of Avian and Human Extraintestinal Pathogenic Escherichia coli as Determined by Genomic Subtractive Hybridization. BMC Microbiol.7:81-88.
*Skyberg, J., Johnson, T.J., and Nolan, L.K. 2008. Mutational and Transcriptional Analyses of an Avian Pathogenic Escherichia coli ColV Plasmid. BMC Microbiol. 8:24.
*Johnson, T.J., Wannemuehler, Y.M., and Nolan, L.K. 2008. Evolution of the iss Gene in Escherichia coliAppl Environ Microbiol. 74:2360-9
*Johnson, T.J., Wannemuehler, Y., Johnson, S.J.,Stell, A.L., Doetkott, C., Johnson, J.R., Kim, K.S., Spanjaard, L., and Nolan, L.K. 2008. Comparison of Extraintestinal Pathogenic Escherichia coli from Human and Avian Sources Reveals a Mixed Subset Representing Potential Zoonotic Pathogens. Appl Environ Microbiol. 74: 7043-7050
*Johnson, T.J., Wannemuehler, Y.M., Doetkott, C., Johnson, S.J., Rosenberger, S.C., and Nolan, L.K. 2008. Identification of Minimal Predictors of Avian Pathogenic Escherichia coli (APEC) Virulence for Use as a Rapid Diagnostic Tool. J Clin Microbiol. EPub Oct. 2008