Efficacy of a pure 0.02% topical hypochlorous acid-containing solution for the treatment of Canine Superficial Pyoderma

Area of Study: 

Purpose of Study: 

Treatment of canine superficial pyoderma has been traditionally based on systemic antibiotics (Cephalexin) administration with topical therapy suggested as extra treatment. However, the continued emergence of resistant infections has renewed interest in topical therapy as a primary means for managing pyoderma with published guidelines now recommending the use of topical antimicrobial shampoos and sprays as a first treatment option.

The aim of this pilot investigation is to evaluate the clinical efficacy of a pure 0.02% hypochlorous acid containing solution applied three times daily compared to an accepted combined topical treatment protocol using a chlorhexidine shampoo and solution to treat dogs with pyoderma. 


Your dog is eligible for this study if

  • He/She has a confirmed clinical diagnosis of superficial pyoderma.
  • The causative agent is confirmed to be Staphylococcus pseudintermedius via microbiologic culture and identification.  

We can't enroll your dog in this study if:
- He/She presents with clinical signs suggestive of deep pyoderma or the presence of ectoparasites and/or Malassezia spp are identified on initial cytologic examinations.
- He/She received systemic antibiotics or topical therapies (except parasitic preventatives) in the preceding 10 days,
- He/She is receiving glucocorticoids or other systemic medications for the treatment of underlying predisposing conditions (i.e. atopic dermatitis or hypothyroidism).

Client Responsibilities: 

If you agree to have your animal participate in this study, the participation will last for 1 month and consist of 3 visits. Each visit will last roughly 1-2 hours.  

During the study, you may expect the following procedures to be used:

  • A physical exam to evaluate the extent and severity of the infection,
  • A skin scrape and cytology to verify the presence of bacteria to determine that this study is appropriate for you pet, 
  • A bacterial culture to confirm the type of bacteria causing the infection.

Following diagnostics your pet will be randomized to one of two treatment groups: 

  • The first involving a spray that you will need to apply to the affected areas three time a day for up to 1 month.
  • The other using a shampoo twice a week and spray once a day on the days that your pet is not bathed.

All treatments are to be performed by you at home. If you elect to participate you will also receive a treatment schedule to help ensure treatments are followed.

Recheck visits will be at 14 and 28 days from the initial visit. At these rechecks, another physical exam and cytology will be done to determine if the therapy is beneficial, resulting in clinical improvement. If at the 14 or 28 day recheck you pet is determined to be worse than at the previous visit, they will be removed from the study and place on more traditional therapy using a systemic antibiotic. 

Client Benefits: 

You will not be responsible for costs from your animal’s participation in this study.

As a result of participation, your pet’s initial visit will be reimbursed and the costs of diagnostics, treatment, and rechecks will be covered by a grant for this investigation. In addition, if your pet is determined to worsen at a recheck visit the alternate traditional therapy will be provided at no cost.

Participation via study completion or removal is needed for reimbursement for the initial visit to be applied to your account.

You will be responsible for any costs associated with the treatment of any unrelated medical conditions.

You will not be compensated for your animal’s participation in this study. 


Dr. Darren Berger: 515-294-4900