Bryan Bellaire

Ph.D., SM (NRCM)
Education & Certifications  
1995   B.S. Microbiology, Northern Arizona University, Flagstaff, AZ.
2001   Ph.D. Microbiology, Louisiana State University Health Sciences Center. Shreveport, LA.
2016 Certified Biosafety Specialist-National Registry of Certified Microbiologists, American Society for Microbiology [(SM)NRCM]
2016 Registered Biosafety Professional [RBP] through American Biological Safety Association

2015 Fall MICRO302 Microbiology

2011-Present: VMPM586L Veterinary Bacteriology 

2007 - Present: Course Co-Organizer VMPM 604

Research Focus & Interests  

Our research is focused on understanding the microbial and cellular events that take place during persistent bacterial infections. Areas of investigation include Bacterial Pathogenesis of Brucella spp. in swine, bovine, canine and human hosts; Translational Medicine  developing novel anti-infective delivery platform to treat persistent bacterial and parasitic infections; Biosafety and Biodefense: illuminating molecular interactions between the human host and virulent Brucella, Francisella, Burkholderia and Yersinia spp.




Nanovaccine Initiative

Honors & Awards  
1994    Undergraduate Fellowship: Science and Engineering Research Semester Sponsored by the Department of Energy. January – May.
1997    Arnold Ravin – Murial Rogers Fellowship. 41st Annual Wind River Conference on Procaryotic Biology. June.
1998    The Donald E. Kahn Memorial Award for the Outstanding Graduate Student Presentation. 79th Annual Conference for Research Workers in Animal Diseases.
2000    McCleskey Award for the Outstanding Graduate Student Presentation in Bacteriology. South Central Branch American Society for Microbiology.
2002    Outstanding Postdoctoral Poster Presentation. Ray A. Barlow Scientific Symposium.
2009    Honorary Member of Phi Zeta Veterinary Honors Society.
2013 Co-Inventor of Antimicrobial Compositions and Methods. US Patent 8,449,916.
2014 Co-Inventor of Antimicrobial Polyanhydride and Methods. US Patent 8,927,024
2015 Co-Inventor of Antiparasitic Polyanhydride Nanoparticles. US Patent Application 14/614,333.
2006-present: ISU VMPM-BSL3 Laboratory Director
2014-present: CVM Safety Committee
American Society for Microbiology
American Association for the Advancement of Science
Selected Publications  

Full publication list on PubMedGoogle Scholar

Davoudi, Z., Peroutka-Bigus, N., Bellaire, B., Wannemuehler, M., Barrett, T. A., Narasimhan, B., Wang, Q. Intestinal organoids containing poly(lactic-co-glycolic acid) nanoparticles for the treatment of inflammatory bowel diseases. J Biomed Mater Res A. 2017 Dec 11. doi: 10.1002/jbm.a.36305

Bussiere, L. D., Choudhury, P., Bellaire, B., Miller, C. L. Characterization of a Replicating Mammalian Orthoreovirus with Tetracysteine-Tagged muNS for Live-Cell Visualization of Viral Factories. J. Virology. November 15, 2017. 10.1128/JVI.01371-17.

Phanse Y, Carrillo-Conde BR, Ramer-Tait RE Roy R, Broderick S, Pohl N, Rajan K, Narasimhan B, Wannemuehler MJ and Bellaire, BH. Functionalization Promotes Pathogen-Mimicking Characteristics of Polyanhydride Nanoparticle Adjuvants. J Biomed Mater Res A. 2017 May. Epub 10.1002/jbm.a.36128

Sponseller BA, Clark SK, Gilbertie J, Wong DM, Hepworth K, Wiechert S, Chandramani P, Sponseller BT, Alcott CJ, Bellaire B, Petersen AC, Jones DE. Macrophage effector responses of horses are influenced by expression of CD154. Vet Immunol Immunopathol. 2016 Nov 1;180:40-44

Phanse Y, Lueth P, Ramer-Tait AE, Carrillo-Conde BR, Narasimhan B, Wannemuehler MJ and Bellaire BH. Cellular Internalization Mechanisms of Polyanhydride Particles: Implications for Rational Design of Drug Delivery Vehicles. J Biomedical Nanotechnology. Vol. 12, 2016.

Jeffery U, Kimura K, Gray R, Lueth P, Bellaire BH, Roth J, LeVine D. Dogs cast NETs too: canine neutrophil extracellular traps in health and immune-mediated hemolytic anemia. Journal of Veterinary Immunology and Immunopathology. 2015 Dec 15;168(3-4):262-8.
B. H. Bellaire, Ph.D.

Ross K, Brenza T, Binnebose A, Phanse Y, Kanthasamy A, Gendelman H, Salem A, Bartholomay L, Bellaire BH, Narasimhan B. Nano-enabled Delivery of Diverse Payloads across Complex Biological Barriers. J Control Release. 2015 Dec 10;219:548-59.

Binnebose A, Martin R, Haughney S, Narasimhan B, Bellaire BH. Polyanhydride nanoparticle delivery platform enables enhanced killing of filarial worms. PLoS Negl Trop Dis. 2015 Oct 23;9(10).

Gibson-Corley KN, Bockenstedt MM, Li H, Boggiatto PM, Phanse Y, Petersen CA, Bellaire BH, and Jones DE. An in vitro model of antibody-enhanced killing of the intracellular parasite Leishmania amazonensis. PLoS One. 2014 Sep 5;9(9).

Boggiatto, P. M., Martinez, P. A., Pullikuth, A., Jones, D. E., Bellaire, B., Catling, A., and Petersen, C. Targeted extracellular signal-regulated kinase activation mediated by Leishmania amazonensis requires MP1 scaffold. Microbes Infect. Jan. 2014.

Kauffman, L. K., Bjork, J. K., Gallup, J. M., Boggiatto, P. M., Bellaire, B. H., and Petersen, C. A. Early detection of Brucella canis via quantitative polymerase chain reaction analysis. Zoonoses Public Health. Feb. 2014. 61, 48-54

Phanse, Y., B. Carillo-Conde, A.E. Ramer-Tait, R. Roychoudhury, N.L. Pohl, B. Narasimhan, M.J. Wannemuehler and B.H. Bellaire. Functionalization of polyanhydride microparticles with di-mannose influences uptake by and intracellular fate within dendritic cells. Acta Biomater. 2013 Jun 21.

Ramer-Tait, AE, S.M. Lei, B.H. Bellaire, and J.K. Beetham. Differential Surface Deposition of Complement Proteins on Logarithmic and Stationary Phase Leishmania chagasi Promastigotes. J Parasitol. 2012. June 4.

Klionsky, DJ. et al. Autophagy. 2012 Apr;8(4):445-544.

Ritchie, J.A., A. Rupper, J.A. Cardelli and B.H. Bellaire. Host Interferon-γ Inducible Protein Contributes to Brucella Survival. Cellular and Infection Microbiology. 2012;2:55.

Phanse, Y., A.E. Ramer-Tait, S.L. Friend, B. Carillo-Conde, P. Lueth. C.J. Oster, G.J. Phillips, B. Narasimhan, M.J. Wannemuehler and B.H. Bellaire. Analyzing cellular internalization of nanoparticles and bacteria by multi-spectral imaging flow cytometry. Journal of Visualized Experiments. 2012 Jun 8;(64):e3884.

Ulery, B.D., L.K. Petersen, Y. Phanse, C.S. Kong, S.R. Broderick, D. Kumar, A.E. Ramer-Tait, B. Carillo-Conde, K. Rajan, M.J. Wannemuehler, B.H. Bellaire, D. Metzger and B. Narasimhan. 2011. Rational Design of Pathogen Mimicking Amphiphilic Materials as Nanoadjuvants. Sci Rep. 2011 Dec 16; 1(198).

Carrillo-Conde, B., A. Chavez-Santoscoy, Y. Phanse, A.E. Ramer-Tait, N.L. Pohl, M.J. Wannemuehler, B.H. Bellaire and B. Narasimhan. Mannose-functionalized "pathogen-like" polyanhydride nanoparticles target C-type lectin receptors on dendritic cells. Mol Pharm. 2011 Oct 3;8(5):1877-86.

Torres, M.P., J.H. Wilson-Welder, S.K. Lopac, Y. Phanse, B. Carrillos-Conde, A.E. Ramer-Tait, B.H. Bellaire, M.J. Wannemuehler, and B. Narasimhan. 2011. Polyanhydride microparticles enhance dendritic cell antigen presentation and activation. Act Bio. Jul; 7(7):2857-64.

Michael Carruthers, BH Bellaire and Chris Minion. 2011. Exploring the response of Escherichia coli O157:H7 EDL933 within Acanthamoeba castellanii by genome-wide transcriptional profiling. FEMS Letters.

Brucellosis in Large Animals. Co-Authors: S.C. Olsen, B.H. Bellaire, R.M. Roop II, and C.O. Thoen. In Pathogenesis of Bacterial Infections in Animals. Ed. Carlton Gyles, John Prescott, Glenn Songer and Charles Thoen.

Ulery BD, Phanse Y, Sinha A, Wannemuehler MJ, Narasimhan B, Bellaire BH. 2009. Polymer chemistry influences monocytic uptake of polyanhydride nanospheres. Pharm Res. Mar;26(3):683-90.

Snider, J.L, B.H. Bellaire and J.A. Cardelli. Both CagA-Dependent and –Independent Signaling Pathways Contribute to Helicobacter pylori – Induced Motility of Gastric Carcinoma Cells. J. Cell Biol. Feb 2007.

Roux, C.M., N.J. Booth, B.H. Bellaire, J.M. Gee, R.M. Roop II, M.E. Kovach, R.M. Tsolis, P.H. Elzer and D.G. Ennis. 2006. RecA and RadA proteins of Brucella abortus do not perform overlapping protective DNA repair functions following oxidative burst. J. Bacteriol. 188(14): 5187-5195.

 B.C. Reed, C. Cefalu, B.H. Bellaire, J.A. Cardelli, T. Louis, J. Salamon, M.A. Bloecher and R.C. Bunn. 2005. GLUT1CBP(TIP2/GIPC1) Interactions with GLUT1 and Myosin VI: Evidence Supporting an Adapter Function for GLUT1CBP Mol. Bol. Cell. 16(9): 4183-4201.

Bellaire, B.H., R.M. Roop II, J.A. Cardelli. 2005. Opsonized virulent Brucella abortus replicate within non-acidic, endoplasmic reticulum negative, LAMP 1 positive phagosomes in human monocytes. Infect. Immun. 73(6): 3702-3713.

Valderas, M.W., R.B. Alcantara, J.B. Baumgartner, B.H. Bellaire, G.T. Robertson, W.L. Ng, J.M. Richardson, M.E. Winkler, and R.M. Roop II. 2005. Role of HdeA in acid resistance and virulence in Brucella abortus 2308. Vet. Micro. 20;107(3-4): 307-12.

Roop II, R.M., B.H. Bellaire, M.W. Valderas, and J.A. Cardelli. 2004. Adaptation of the brucellae to their intracellular niche. Mol. Micro. 52(3): 621-630.

RoopII, R.M., B.H. Bellaire, E. Anderson and J.T. Paulley. 2004. Iron metabolism in Brucella, p. 243-262. In I. Lopez-Goni and I. Moriyon (ed.), Brucella: Molecular and cellular biology. Horizon Scientific Press.

Bellaire, B.H., P.H. Elzer, C.L. Baldwin, and R.M. Roop II. 2003. Production of the siderophore 2,3-dihydroxybenzoic acid is required for wild-type growth of Brucella abortus in the presence of erythritol under low iron conditions in vitro. Infect Immun. 71(5): 2927-832.

Bellaire, B.H., P.H. Elzer, S.D. Hagius, J.V. Walker, N.J. Booth, M.D. Edmonds, C.L. Baldwin, R. Freeland and R.M. Roop II. 2003. Genetic organization and iron-Responsive regulation of the Brucella abortus 2,3-dihydroxybenzoic acid biosynthesis operon, a cluster of genes required for wild-type virulence in pregnant cattle. Infect Immun. 71(4): 1794-1803.

Parent, M.A., Bellaire, B.H., Murphy, E.A., R.M. Roop II, P.H. Elzer and C.L. Baldwin. 2002. Brucella abortus siderophore 2,3-dihydroxybenzoic acid (DHBA) facilitates intracellular survival of the bacteria. Microb. Pathog. 32(5): 239-248.

Bellaire, B.H., C.L. Baldwin, P.H. Elzer and R.M. Roop II. 1999. The siderophore 2,3-dihydroxybenzoic acid is not required for virulence of Brucella abortus in BALB/c mice. Infect. Immun. 67: 2615-2618.