Alan P Robertson

Ph.D.
Associate Professor
515-294-1212
2058 Vet Med
Education & Certifications  

• Ph.D., Veterinary Medicine (Invertebrate Neuroscience), 1997, Edinburgh University, UK

• B.Sc., Parasitology, 1991, University of Glasgow, UK

Teaching  

  • 2012-present   Associate Professor, Professor. Dept Biomedical Sciences, College of Vet Med. Iowa State University. Independent & Collaborative research on nematode ion channels and anthelmintic drug development

     

  • 2010-2012       Research Associate Professor. Dept Biomedical Sciences, College of Vet Med. Iowa State University. Independent & Collaborative research on nematode ion channels and anthelmintic drug development

     

  • 2006-2009       Affiliate assistant professor.  Dept Biomedical Sciences, College of Vet. Med.  Iowa State University, Ames, IA.  Managing electrophysiology /molecular biology lab supervising the following projects, (i) single-channel study of nicotinic receptor heterogeneity in Ascaris suum, (ii) role of phosphorylation in modulating whole cell responses to cholinergic anthelmintics on Ascaris suum, (iii)  comparison of nicotinic receptor subunits in anthelmintic sensitive and resistant isolates of O. dentatum, (iv) modulation of nematode neuromuscular transmission by endogenous peptides, (v) patch-clamp investigation of C. elegans nicotinic acetylcholine receptors.

     

  • 2001 -2006      Adjunct assistant professor.  Dept Biomedical Sciences, College of Vet. Med.  Iowa State University, Ames, IA.  Managing electrophysiology /molecular biology lab supervising the following projects, (i) single-channel study of nicotinic receptor heterogeneity in Ascaris suum, (ii) role of phosphorylation in modulating whole cell responses to cholinergic anthelmintics on Ascaris suum, (iii)  comparison of nicotinic receptor subunits in anthelmintic sensitive and resistant isolates of O. dentatum, (iv) modulation of nematode neuromuscular transmission by endogenous peptides, (v) patch-clamp investigation of C. elegans nicotinic acetylcholine receptors.

     

  • 1999-2000       Postdoctoral Fellow. Dept Preclinical Vet. Sciences, Edinburgh University, UK.  Project entitled “Modulation of levamisole receptors by protein kinases and phosphatases and anthelmintic resistance in Oesophagostomum dentatum".
  • 1997-1999       Postdoctoral researcher. Dept Preclinical Vet. Sciences, Edinburgh University, UK. Project entitled “A patch-clamp analysis of changes in levamisole receptors associated with anthelmintic resistance”.

     

  • 1996-1997       Research Associate. Dept Preclinical Vet. Sciences, Edinburgh University, UK. Project entitled “A patch-clamp analysis of changes in levamisole receptors associated with anthelmintic resistance”.

     

Research Focus & Interests  

Nematode ion channels as anti-parasitic drug targets. Electrophysiology of ligand- and voltage-gated ion channels in nematode parasites and C. elegans. Identifying anti-nematodal modes of action and mechanisms of resistance to anthelmintics. Modulation of ionotropic acetylcholine receptors at the nematode neuromuscular junction. Characterization of novel ion channels and validation as potential drug targets.

Selected Publications  

Link to PubMed

1: Abongwa M, Buxton SK, Robertson AP, Martin RJ. (2016) Curiouser and Curiouser: The

Macrocyclic Lactone, Abamectin, Is also a Potent Inhibitor of Pyrantel/Tribendimidine Nicotinic Acetylcholine Receptors of Gastro-Intestinal Worms. PLoS One. Jan 11;11(1):e0146854. doi: 10.1371/journal.pone.0146854. eCollection. PubMed PMID: 26751958; PubMed Central PMCID: PMC4709073.

 

2: McCoy CJ, Warnock ND, Atkinson LE, Atcheson E, Martin RJ, Robertson AP, Maule

AG, Marks NJ, Mousley A. (2015) RNA interference in adult Ascaris suum--an opportunity

for the development of a functional genomics platform that supports organism-,

tissue- and cell-based biology in a nematode parasite. Int J Parasitol. Sep;45(11):673-8. doi: 10.1016/j.ijpara.2015.05.003. Epub 2015 Jul 4. PubMed PMID: 26149642; PubMed Central PMCID: PMC4563943.

 

3: Martin RJ, Verma S, Choudhary S, Kashyap S, Abongwa M, Zheng F, Robertson AP.

(2015) Anthelmintics: The best way to predict the future is to create it. Vet Parasitol.

Aug 15;212(1-2):18-24. doi: 10.1016/j.vetpar.2015.05.016. Epub 2015 Jun 20.

PubMed PMID: 26138153; PubMed Central PMCID: PMC4530025.

 

4: Trailović SM, Marjanović DS, Nedeljković Trailović J, Robertson AP, Martin RJ. (2015)

Interaction of carvacrol with the Ascaris suum nicotinic acetylcholine receptors

and gamma-aminobutyric acid receptors, potential mechanism of antinematodal

action. Parasitol Res. Aug;114(8):3059-68. doi: 10.1007/s00436-015-4508-x. Epub 2015 May 7. PubMed PMID: 25944741; PubMed Central PMCID: PMC4607277.

 

5: Robertson AP, Puttachary S, Buxton SK, Martin RJ. (2015) Tribendimidine: mode of

action and nAChR subtype selectivity in Ascaris and Oesophagostomum. PLoS Negl

Trop Dis. Feb 13;9(2):e0003495. doi: 10.1371/journal.pntd.0003495. eCollection 2015 Feb. PubMed PMID: 25679515; PubMed Central PMCID: PMC4334517.

 

6: Kotze AC, Hunt PW, Skuce P, von Samson-Himmelstjerna G, Martin RJ, Sager H,

Krücken J, Hodgkinson J, Lespine A, Jex AR, Gilleard JS, Beech RN, Wolstenholme

AJ, Demeler J, Robertson AP, Charvet CL, Neveu C, Kaminsky R, Rufener L, Alberich

M, Menez C, Prichard RK. (2014) Recent advances in candidate-gene and whole-genome

approaches to the discovery of anthelmintic resistance markers and the description of drug/receptor interactions. Int J Parasitol Drugs Drug Resist. Aug 13;4(3):164-84. doi: 10.1016/j.ijpddr.2014.07.007. eCollection 2014 Dec.Review. PubMed PMID: 25516826; PubMed Central PMCID: PMC4266812.

 

7: Buxton SK, Robertson AP, Martin RJ. (2014) Diethylcarbamazine increases activation of

voltage-activated potassium (SLO-1) currents in Ascaris suum and potentiates effects of emodepside. PLoS Negl Trop Dis. Nov 20;8(11):e3276. doi:10.1371/journal.pntd.0003276. eCollection 2014 Nov. PubMed PMID: 25411836; PubMed Central PMCID: PMC4238981.

 

8: MacDonald K, Buxton S, Kimber MJ, Day TA, Robertson AP, Ribeiro P. (2014) Functional characterization of a novel family of acetylcholine-gated chloride channels in

Schistosoma mansoni. PLoS Pathog. Jun 12;10(6):e1004181. doi:10.1371/journal.ppat.1004181. eCollection 2014 Jun. PubMed PMID: 24945827; PubMed Central PMCID: PMC4055736.

 

9: Chehayeb JF, Robertson AP, Martin RJ, Geary TG. (2014) Proteomic analysis of adult

Ascaris suum fluid compartments and secretory products. PLoS Negl Trop Dis. Jun 5;8(6):e2939. doi: 10.1371/journal.pntd.0002939. eCollection  Jun. PubMed

PMID: 24901219; PubMed Central PMCID: PMC4046973.

 

10: Martin RJ, Puttachary S, Buxton SK, Verma S, Robertson AP. (2015) The Conqueror

Worm: recent advances with cholinergic anthelmintics and techniques excite research for better therapeutic drugs. J Helminthol. Jul;89(4):387-97. doi: 10.1017/S0022149X1400039X. Epub 2014 May 29. PubMed PMID: 24871674; PubMed Central PMCID: PMC4247809.

 

11: Buxton SK, Charvet CL, Neveu C, Cabaret J, Cortet J, Peineau N, Abongwa M,

Courtot E, Robertson AP, Martin RJ. (2014) Investigation of acetylcholine receptor

diversity in a nematode parasite leads to characterization of tribendimidine- and

derquantel-sensitive nAChRs. PLoS Pathog. Jan 30;10(1):e1003870. doi: 10.1371/journal.ppat.1003870. eCollection 2014 Jan. PubMed PMID: 24497826; PubMed

Central PMCID: PMC3907359.

 

12: Robertson AP, Buxton SK, Martin RJ. (2013 ) Whole-cell patch-clamp recording of

nicotinic acetylcholine receptors in adult Brugia malayi muscle. Parasitol Int. Dec;62(6):616-8. doi: 10.1016/j.parint.2013.03.008. Epub 2013 Apr 3. PubMed PMID: 23562945; PubMed Central PMCID: PMC3744629.

 

13: Puttachary S, Trailovic SM, Robertson AP, Thompson DP, Woods DJ, Martin RJ. (2013) Derquantel and abamectin: effects and interactions on isolated tissues of Ascaris suum. Mol Biochem Parasitol. Apr;188(2):79-86. doi: 10.1016/j.molbiopara.2013.02.004. Epub 2013 Mar 21. PubMed PMID: 23523993; PubMed Central PMCID: PMC3864555.

 

14: Martin RJ, Robertson AP, Buxton SK, Beech RN, Charvet CL, Neveu C. (2012) Levamisole receptors: a second awakening. Trends Parasitol. Jul;28(7):289-96. doi:

10.1016/j.pt.2012.04.003. Epub 2012 May 17. Review. PubMed PMID: 22607692; PubMed

Central PMCID: PMC3378725.

 

15: Charvet CL, Robertson AP, Cabaret J, Martin RJ, Neveu C. (2012) Selective effect of

the anthelmintic bephenium on Haemonchus contortus levamisole-sensitive acetylcholine receptors. Invert Neurosci. Jun;12(1):43-51. doi:10.1007/s10158-012-0130-0. Epub 2012 Apr 24. PubMed PMID: 22526556; PubMed Central PMCID: PMC3362318.

 

16: Martin RJ, Buxton SK, Neveu C, Charvet CL, Robertson AP. (2012) Emodepside and SL0-1 potassium channels: a review. Exp Parasitol. Sep;132(1):40-6. doi: 10.1016/j.exppara.2011.08.012. Epub 2011 Sep 3. Review. PubMed PMID: 21910990;

PubMed Central PMCID: PMC3262936.

 

17: Carr JA, Parashar A, Gibson R, Robertson AP, Martin RJ, Pandey S. (2011) A microfluidic platform for high-sensitivity, real-time drug screening on C. elegans and parasitic nematodes. Lab Chip. Jul 21;11(14):2385-96. doi: 10.1039/c1lc20170k. Epub 2011 Jun 6. PubMed PMID: 21647497; PubMed Central PMCID: PMC3645292.

 

18: Robertson AP, Puttachary S, Martin RJ. (2011)Single-channel recording from adult Brugia malayi. Invert Neurosci. Jun;11(1):53-7. doi: 10.1007/s10158-011-0118-1. Epub 2011 May 18. PubMed PMID: 21590329.

 

19: Buxton SK, Neveu C, Charvet CL, Robertson AP, Martin RJ. (2011) On the mode of

action of emodepside: slow effects on membrane potential and voltage-activated currents in Ascaris suum. Br J Pharmacol. Sep;164(2b):453-70. doi: 10.1111/j.1476- 381.2011.01428.x. PubMed PMID: 21486286; PubMed Central PMCID: PMC3188918.

 

20: Martin RJ, Robertson AP (2010) Control of nematode parasites with agents acting on

neuro-musculature systems: lessons for neuropeptide ligand discovery. Adv Exp Med

Biol. 2010;692:138-54. Review. PubMed PMID: 21189677; PubMed Central PMCID:

PMC3732103.

 

21: McVeigh P, Kimber MJ, Novozhilova E, Atkinson L, Robertson AP, Zamanian M,

Marks NJ, Maule AG, Day TA. (2010) Neuropeptide signalling in platyhelminths. Regul Pept. Sep 9;164(1):19-20. doi: 10.1016/j.regpep.2010.07.134. Epub 2010 Aug 20. PubMed PMID: 26009365.

 

22: Novozhilova E, Kimber MJ, Qian H, McVeigh P, Robertson AP, Zamanian M, Maule

AG, Day TA.(2010) FMRFamide-like peptides (FLPs) enhance voltage-gated calcium currents to elicit muscle contraction in the human parasite Schistosoma mansoni. PLoS Negl

Trop Dis. Aug 10;4(8):e790. doi: 10.1371/journal.pntd.0000790. PubMed PMID: 20706630; PubMed Central PMCID: PMC2919380.

 

23: Chen B, Deutmeyer A, Carr J, Robertson AP, Martin RJ, Pandey S.  (2011) Microfluidic

bioassay to characterize parasitic nematode phenotype and anthelmintic resistance. Parasitology. Jan;138(1):80-8. doi: 10.1017/S0031182010001010. Epub 2010 Jul 21. PubMed PMID: 20663251; PubMed Central PMCID: PMC3005416.

 

24: Puttachary S, Robertson AP, Clark CL, Martin RJ. (2010) Levamisole and ryanodine receptors. II: An electrophysiological study in Ascaris suum. Mol Biochem Parasitol. May;171(1):8-16. doi: 10.1016/j.molbiopara..12.006. Epub 2010 Jan 11. PubMed PMID: 20064567; PubMed Central PMCID: PMC2839013.

 

25: Robertson AP, Clark CL, Martin RJ. (2010) Levamisole and ryanodine receptors. I: A contraction study in Ascaris suum. Mol Biochem Parasitol. May;171(1):1-7. doi: 10.1016/j.molbiopara.2009.12.007. Epub 2010 Jan 11. PubMed PMID: 20064566; PubMed Central PMCID: PMC2838958.

 

26: Williamson SM, Robertson AP, Brown L, Williams T, Woods DJ, Martin RJ, Sattelle DB, Wolstenholme AJ. (2009) The nicotinic acetylcholine receptors of the parasitic nematode Ascaris suum: formation of two distinct drug targets by varying the relative expression levels of two subunits. PLoS Pathog. Jul;5(7):e1000517. doi: 10.1371/journal.ppat.1000517. Epub 2009 Jul 17. PubMed PMID: 19609360; PubMed Central PMCID: PMC2705655.

 

27: Robertson AP, Puttachary S, Buxton SK, Martin RJ.  (2008) Electrophysiological recording from parasitic nematode muscle. Invert Neurosci. Dec;8(4):167-75. doi: 0.1007/s10158-008-0080-8. Epub 2008 Nov 13. PubMed PMID: 19005711; PubMed Central PMCID: PMC2772203.

 

28: Verma S, Robertson AP, Martin RJ. (2009) Effects of SDPNFLRF-amide (PF1) on

voltage-activated currents in Ascaris suum muscle. Int J Parasitol. Feb;39(3):315-26. doi: 0.1016/j.ijpara.2008.07.007. Epub 2008 Aug 12. PubMed PMID: 18760280; PubMed Central PMCID: PMC2632723.

 

29: Qian H, Robertson AP, Powell-Coffman JA, Martin RJ. (2008) Levamisole resistance resolved at the single-channel level in Caenorhabditis elegans. FASEB J. Sep;22(9):3247-54. doi: 10.1096/fj.08-110502. Epub 2008 Jun 2. PubMed PMID: 18519804; PubMed Central PMCID: PMC2518249.

 

30: Trailovic SM, Verma S, Clark CL, Robertson AP, Martin RJ. (2008) Effects of the muscarinic agonist, 5-methylfurmethiodide, on contraction and electrophysiology of Ascaris suum muscle. Int J Parasitol. Jul;38(8-9):945-57. doi: 10.1016/j.ijpara.2007.11.011. Epub 2007 Dec 8. PubMed PMID: 18206155; PubMed Central PMCID: PMC2485209.

 

31: Robertson AP, Martin RJ. (2007) Ion-channels on parasite muscle: pharmacology and physiology. Invert Neurosci. Dec;7(4):209-17. Epub 2007 Nov 13. Review. PubMed PMID: 17999098.

 

32: Martin RJ, Robertson AP. (2007) Mode of action of levamisole and pyrantel, anthelmintic resistance, E153 and Q57. Parasitology. 2007;134(Pt 8):1093-104. Review. PubMed PMID: 17608969.

 

33: Verma S, Robertson AP, Martin RJ.  (2007) The nematode neuropeptide, AF2 (KHEYLRF-NH2), increases voltage-activated calcium currents in Ascaris suum muscle. Br J Pharmacol. Jul;151(6):888-99. Epub 2007 May 21. PubMed PMID: 17519945; PubMed Central PMCID: PMC2014128.

 

34: Qian H, Martin RJ, Robertson AP. (2006) Pharmacology of N-, L-, and B-subtypes of nematode nAChR resolved at the single-channel level in Ascaris suum. FASEB J. Dec;20(14):2606-8. Epub 2006 Oct 20. PubMed PMID: 17056760.

 

35: Martin RJ, Verma S, Levandoski M, Clark CL, Qian H, Stewart M, Robertson AP. (2005) Drug resistance and neurotransmitter receptors of nematodes: recent studies on the mode of action of levamisole. Parasitology.;131 Suppl:S71-84. Review. PubMed PMID: 16569294.

 

36: Levandoski MM, Robertson AP, Kuiper S, Qian H, Martin RJ. (2005) Single-channel properties of N- and L-subtypes of acetylcholine receptor in Ascaris suum. Int J Parasitol. Jul;35(8):925-34. PubMed PMID: 15950977.

 

37: Trailovic SM, Clark CL, Robertson AP, Martin RJ. (2005) Brief application of AF2 produces long lasting potentiation of nAChR responses in Ascaris suum. Mol Biochem Parasitol. Jan;139(1):51-64. PubMed PMID: 15610819.

 

38: Martin RJ, Clark CL, Trailovic SM, Robertson AP. (2004) Oxantel is an N-type (methyridine and nicotine) agonist not an L-type (levamisole and pyrantel) agonist: classification of cholinergic anthelmintics in Ascaris. Int J Parasitol. Aug;34(9):1083-90. PubMed PMID: 15313135.

 

39: Martin RJ, Bai G, Clark CL, Robertson AP. (2003) Methyridine (2-[2-methoxyethyl]-pyridine]) and levamisole activate different ACh receptor subtypes in nematode parasites: a new lead for levamisole-resistance. Br J Pharmacol. 2003 Nov;140(6):1068-76. Epub 2003 Oct 27. PubMed PMID: 14581174; PubMed Central PMCID: PMC1574116.

 

40: Purcell J, Robertson AP, Thompson DP, Martin RJ. (2002) PF4, a FMRFamide-related

peptide, gates low-conductance Cl(-) channels in Ascaris suum. Eur J Pharmacol. Dec 5;456(1-3):11-7. PubMed PMID: 12450564.

 

41: Trailovic SM, Robertson AP, Clark CL, Martin RJ. (2002) Levamisole receptor phosphorylation: effects of kinase antagonists on membrane potential responses in Ascaris suum suggest that CaM kinase and tyrosine kinase regulate sensitivity to levamisole. J Exp Biol. Dec;205(Pt 24):3979-88. PubMed PMID: 12432019.

 

42: Robertson AP, Clark CL, Burns TA, Thompson DP, Geary TG, Trailovic SM, Martin

RJ. (2002) Paraherquamide and 2-deoxy-paraherquamide distinguish cholinergic receptor

subtypes in Ascaris muscle. J Pharmacol Exp Ther. Sep;302(3):853-60. Erratum in: J Pharmacol Exp Ther Nov;303(2):888. PubMed PMID: 12183640.

 

43: Purcell J, Robertson AP, Thompson DP, Martin RJ. (2002) The time-course of the response to the FMRFamide-related peptide PF4 in Ascaris suum muscle cells indicates direct gating of a chloride ion-channel. Parasitology. Jun;124(Pt 6):649-56. PubMed PMID: 12118721.

 

44: Martin RJ, Robertson AP. (2000)Electrophysiological investigation of anthelmintic

resistance. Parasitology.;120 Suppl:S87-94. Review. PubMed PMID: 10874712.

 

45: Robertson AP, Bjørn HE, Martin RJ. (2000) Pyrantel resistance alters nematode nicotinic acetylcholine receptor single-channel properties. Eur J Pharmacol. Apr 7;394(1):1-8. PubMed PMID: 10771027.

 

46: Robertson AP, Bjorn HE, Martin RJ. (1999) Resistance to levamisole resolved at the

single-channel level. FASEB J. Apr;13(6):749-60. PubMed PMID: 10094935.

 

47: Martin RJ, Murray I, Robertson AP, Bjorn H, Sangster N. (1998) Anthelmintics and ion-channels: after a puncture, use a patch. Int J Parasitol. Jun;28(6):849-62. Review. PubMed PMID: 9673865.

 

48: Robertson AP, Martin RJ, Kusel JR. (19997) A vesicle preparation for resolving single-channel currents in tegument of male Schistosoma mansoni. Parasitology. Aug;115 (Pt 2):183-92. PubMed PMID: 10190174.

 

49: Martin RJ, Robertson AP, Bjorn H, Sangster NC. (1997) Heterogeneous levamisole receptors: a single-channel study of nicotinic acetylcholine receptors from Oesophagostomum dentatum. Eur J Pharmacol. Mar 19;322(2-3):249-57. PubMed PMID: 9098695.

 

50: Martin RJ, Robertson AP, Bjorn H. (1997) Target sites of anthelmintics. Parasitology.;114 Suppl:S111-24. Review. PubMed PMID: 9309773.

 

51: Robertson AP, Martin RJ. (1996) Effects of pH on a high conductance Ca-dependent

chloride channel: a patch-clamp study in Ascaris suum. Parasitology. Aug;113 ( Pt 2):191-8. PubMed PMID: 8760318.

 

52: Martin RJ, Valkanov MA, Dale VM, Robertson AP, Murray I. (1996) Electrophysiology of

Ascaris muscle and anti-nematodal drug action. Parasitology.;113 Suppl:S137-56.  review. PubMed PMID: 9051932.

 

53: Redman CA, Robertson A, Fallon PG, Modha J, Kusel JR, Doenhoff MJ, Martin RJ. (1996) Praziquantel: an urgent and exciting challenge. Parasitol Today. Jan;12(1):14-20. PubMed PMID: 15275303.

 

Book Chapters (selected)

R.J. Martin, A.P. Robertson, M. A. Valkanov, J. Purcell and C.S. Lowden.  (2001). Neurobiology of Nematode Muscle: Ligand-gated Ion-Channels and Anti-Parasitic Drugs.  In: Parasitic Nematodes. Editors: M. Kennedy and W Harnett. .  CAB International, Wallingford, U.K. Chapter 21. P 441-447.

 

R. J. Martin, J. Purcell, A. P. Robertson and M. A. Valkanov. (2002) Neuromuscular Organisation and Control. In: The Biology of  Nematodes.    Editor  D. Lee. Taylor and Francis, London and New York. Chapter 12. P 321 – 343

 

Martin, R.J.  Alan P. Robertson, Adrian W. Wolstenholme (2002) Mode of action of the Macrocyclic Lactones. In: Macrocyclic Lactones in Antiparasitic Therapy.  Eds Verrruysse, J. and Rew, R. CAB International.  Wallingford, U.K. & New York. 2002. P125-140

 

Martin, R.J. Purcell, J.  Day. T & Robertson, A. P. (2003)  Neurotransmitters.  Chapter 15. In:  Marr J. and Komuneucki, R. Biochemistry of Parasites.    RE: Molecular Medical Parasitology.  Edited by J.J. Marr, T. Nielsen and R. Komuniecki P 349 – 394.

Debra J. Woods, Steven J. Maeder. Alan P. Robertson, Richard J. Martin, Timothy G. Geary, David P. Thompson, Sandra S. Johnson and George A. Conder. (2012). The Discovery, Mode-of-Action and Commercialization of Derquantel. IN: Parasitic Helminths: Targets, Screens, Drugs, and Vaccines.  Eds  Caffrey & Selzner.  Series Drug Discovery in Infectious Diseases. Wiley & Blackwell.

Alan P. Robertson, Samuel K. Buxton, Sreekanth Puttachary, Sally M. Williamson, Adrian J. Wolstenholme,  Cedric Neveu, Jacques Cabaret, Claude L. Charvet, Richard J. Martin . Anti-nematodal drugs, modes of action and resistance: And worms will not come to thee (2012). IN: Parasitic Helminths: Targets, Screens, Drugs, and Vaccines.  Eds  Caffrey & Selzner.  Series Drug Discovery in Infectious Diseases. Wiley & Blackwell

 

 

Invited Lectures

Anti-nematodal drugs in veterinary medicine, The 15th Symposium in Animal Clinical Pathology and Therapy. "Clinica veterinaria 2013" Beograd, 24th-26th May, 2013

“Cholinergic receptors on the Ascaris suum pharynx” at Anthelmintics from Discovery to Resistance, Fort Mason Conference Center, San Francisco, Feb 5th-7th

“Nematode nicotinic receptors as drug targets” Invited presentation to the Biotechnology Unit, University of Buea, Buea, Cameroon, summer 2014

Update on anthelmintic Resistance: Cholinergic Drugs. Consortium on Anthelmintic Resistance SNPs. Liverpool UK, August 2015.

On the mode of action of new anthelmintics. Special Lecture to Veterinary Students, College of Veterinary Medicine, University of Belgrade, Serbia, May 2015.

 

Conference papers (selected)

Robertson, A. P., Martin, R. J. and Kusel, J.R. (1996).  Single-channel recordings from tegument-derived vesicles of Schistosoma mansoni. Journal of Physiology (London)495P. 33-34P

Robertson, A. P., Bjorn, H., Sangster, N.C. and Martin, R.J. (1996).  Levamisole-activated single channel currents form isolated muscle vesicles of Oesphagostomum dentatum. Journal of Physiology (London). 495P. 83-84

Martin, R. J., A. P. Robertson and  Bjorn, H.  (1997).  Heterogeneous levamisole receptors in Oesophagostomum dentatum and anthelmintic resistance.  British Journal of Pharmacology.

R. Martin, G. Bai, S. Trailovic, C. Clark and A. Robertson, (2002) levamisole receptor phosphorylation: effects of kinase antagonists in Ascaris suum suggest Cam kinase and tyrosine kinase regulates sensitivity to levamisole and could be involved in drug resistance. I International Conference on Parasitology X, Vancuover.

Qian, H. Robertson, A.P. Geary, T.G. Martin R.J (2002). Effects of paraherquamide on nematode nicotinic acetylcholine receptors observed at the single-channel level.  Society for Neuroscience Meeting.  Orlando. 

R.J. Martin, C.L. Clark and A. P. Robertson (2002) Selective changes in cholinergic receptor subtypes associated with levamisole resistance in O. dentatum.  American Association of Veterinary Parasitologists, New Orleans.

H. Qian,  A.P. Robertson, R.J. Martin (2003) Selective effects of paraherquamide reveal the subtype diversity of cholinergic receptors located on nematode somatic muscle, Society for Neuroscience, New Orleans. 

Levandoski, M., Robertson, A.P., Martin, R.J. Biophysically Distinct Acetylcholine Receptor Subtypes in Ascaris Suum Muscle (2003) Society for Neuroscience. New Orleans.

Verma S, Robertson AP & Martin RJ (2006) Calcium currents in somatic muscle cells of Ascaris suum. International Conference on Parasitology XI, Glasgow, UK.

Martin RJ, Robertson AP, Qian H, Verma S & Clark CL (2006) Motor function, drug targets and control: Cholinergic receptors (Keynote Abstract). International Conference on Parasitology XI, Glasgow, UK.

Qian H, Robertson AP & Martin RJ (2006) Comparison of levamisole receptor properties in C. elegans and parasitic nematodes. International Conference on Parasitology XI, Glasgow, UK.

Thomas Boulin1, Georgia Rapti1,Valérie Robert1, Alan Robertson2, Janet Richmond3, Jean-Louis Bessereau1 A novel synaptic protein modulates cholinergic signaling at the C. elegans neuromuscular junction.. 1) ENS INSERM, Paris; 2) Iowa State University; 3) University of Illinois at Chicago. 16th International C. elegans meeting, University of California, Los Angeles

Qian, H., Robertson, A.P., Powell-Coffman, J.A. & Martin R.J. (2007) Changes in C. elegans levamisole receptor single-channel properties in lev-1 and lev-8 mutants. 16th International C. elegans meeting, University of California, Los Angeles

Verma, S., Robertson, A.P. and Martin, R.J. (2007). The neuropeptide PF1, decreaeses increases voltage-activated calcium currents in Ascaris suum muscle.. American Society for Veterinary Parasitology.

SK Buxton, CL Neveu, C Charvet, AP Robertson, RJ Martin “Heterogeneity of nematode nicotinic acetylcholine receptors revealed by functional expression of O.dentatum levamisole receptors in Xenopus oocytes”. Health Sciences Research Week U of I.

 

M. Abongwa, S. K. Buxton, S. Verma, S. Choudhary, M. A. Beltran, A. P. Robertson and R. J. Martin "Pharmacological and electrophysiological characterization of Ascaris suum homopentameric nicotinic acetylcholine receptors in Xenopus laevis oocytes". Central States Regional Chapter Of The Society of Toxicology (CS-SOT) Annual Fall Meeting, held at the Gateway Center and Hotel, Ames, Iowa, U.S.A, October 10-11, 2013. pp33.

S. K. Buxton, A. P. Robertson and R. J. Martin "Nicotinic acetylcholine receptors: expression in oocytes demonstrates functional diversity in parasitic nematodes". Central States Regional Chapter Of The Society of Toxicology (CS-SOT) Annual Fall Meeting, held at the Gateway Center and Hotel, Ames, Iowa, U.S.A, October 10-11, 2013. pp12.

Ma. Asuncion G. Beltran, S. Choudhary, A. P. Robertson, R. J. Martin "A larval migration assay for testing effects of and resistance to anti-parasitic drugs". Central States Regional Chapter Of The Society of Toxicology (CS-SOT) Annual Fall Meeting, held at the Gateway Center and Hotel, Ames, Iowa, U.S.A, October 10-11, 2013. pp21.

S. Puttachary, A.J. Wolstenholme, H.M. Bennett, S.M. Williamson, R.J. Martin & A. P. Robertson "Cholinergic receptors on the nematode pharynx; an unexploited drug target". Central States Regional Chapter Of The Society of Toxicology (CS-SOT) Annual Fall Meeting, held at the Gateway Center and Hotel, Ames, Iowa, U.S.A, October 10-11, 2013. pp25.

S. Verma, A.P. Robertson, S.K. Buxton and R. J. Martin "Brugia malayi a model for comparative study of acetylcholine receptor pharmacology in parasitic nematodes". Central States Regional Chapter Of The Society of Toxicology (CS-SOT) Annual Fall Meeting, held at the Gateway Center and Hotel, Ames, Iowa, U.S.A, October 10-11, 2013. pp35.

Effects of paraherquamide, derquantel and the macrocyclic lactone abamectin, on native and expressed receptors of nematode parasites. RJ Martin, M Abongwa, SK Buxton & AP Robertson at Anthelmintics from Discovery to Resistance, Fort Mason Conference Center, San Francisco, Feb 5th-7th2014

 

C. elegans as an expression system for drug targets from parasitic nematodes. M Sloan, BJ Reaves, AP Robertson & AJ Wolstenholme at Anthelmintics from Discovery to Resistance, Fort Mason Conference Center, San Francisco, Feb 5th-7th 2014

 

Characterization of the Ascaris suum homomeric nicotinic acetylcholine receptor in Xenopus laevis oocytes. M Abongwa, SK Buxton, CL Charvet, C Neveu, AP Robertson & RJ Martin at Anthelmintics from Discovery to Resistance, Fort Mason Conference Center, San Francisco, Feb 5th-7th 2014

 

Nicotinic acetylcholine receptors: expression in oocytes and functional diversity in parasitic nematodes. SK Buxton, CL Charvet, C Neveu, AP Robertson & RJ Martin at Anthelmintics from Discovery to Resistance, Fort Mason Conference Center, San Francisco, Feb 5th-7th 2014

 

Acetylcholine receptor pharmacology in the parasitic nematode Brugia Malayi. S Verma, AP Robertson & RJ Martin at Anthelmintics from Discovery to Resistance, Fort Mason Conference Center, San Francisco, Feb 5th-7th 2014

 

 Ascaris suum ACR-21 forms a homomeric nicotinic acetylcholine receptor with novel pharmacology. S Choudary, M Abongwa, SK Buxton, CJ McCoy, AP Robertson, S Verma & RJ Martin at American Association of Veterninry Parasitologists annual meeting, Denver, Co, July-26-29 2014

 

Not just Glu-Cls: abamectin has potent effects on nicotinic acetylcholine receptors of nematode parasites. RJ Martin, M Abongwa, SK Buxton & AP Robertson at American Association of Veterninry Parasitologists annual meeting, Denver, Co, July-26-29 2014

 

Electrophysiological effects of cholinergic anthelmintics on two species of filarial parasite. S Verma, AP Robertson, AJ Wolstenholme & RJ Martin at American Association of Veterninry Parasitologists annual meeting, Denver, Co, July-26-29 2014

 

Characterization of a homomeric nicotinic acetylcholine receptor, Ascaris suum ACR-16 in Xenopus laevis oocytes. AP Robertson, M Abongwa, SK Buxton, E Courtot, C Charvet, C Neveu & RJ Martin at American Association of Veterninry Parasitologists annual meeting, Denver, Co, July-26-29 2014

 

“Pharmacological Characterization of ionotropic receptors in filarial parasite, Brugia malayi.” S. Verma, A.P. Robertson & R.J. Martin. American Association of Veterinary Parasitologists Annual Meeting July 11th-14th Boston, Ma.

 

“A novel pharyngeal receptor formed by EAT-2 and EAT-18 as a potential drug target”. A.P. Robertson, S Choudhary, S. Buxton, RJ Martin. American Association of Veterinary Parasitologists Annual Meeting July 11th-14th Boston, Ma.

 

Anthelmintic activity and metabolomic profiling of extracts of Daniellia oliveri and Psorospermum febrifugum. Melanie Abongwa1*, Fidelis Cho-Ngwa2, Godfred A. Ayimele3, Moses Samje2, Smith B. Babiaka3, Ann Perera4, Richard J. Martin1, Alan P. Robertson1. World Association for the Advancement of Veterinary Parasitology Conference, August 2015, Liverpool UK.

 

Novel drug targets in filarial nematodes – is slow an option? Verma Saurabh, Robertson Alan P, and Martin Richard J. World Association for the Advancement of Veterinary Parasitology Conference, August 2015, Liverpool UK.

 

Not just GluCls (Glutamate-gated Chloride channels): Abamectin has potent effects on nicotinic acetylcholine receptors of nematode parasites. Melanie Abongwa, Alan P. Robertson, Richard J. Martin. World Association for the Advancement of Veterinary Parasitology Conference, August 2015, Liverpool UK.

 

Asu‐ACR‐16: a homomeric nAChR drug target widely distributed in Ascaris tissues. Melanie Abongwa1*, Fudan Zheng2*, Samuel K. Buxton1, Elise Courtot3,4, Claude Charvet3,4, Cédric Neveu3,4, Ciaran J. McCoy5, Saurabh Verma1, Edward Yu2, Richard J. Martin1 and Alan. P. Robertson1. World Association for the Advancement of Veterinary Parasitology Conference, August 2015, Liverpool UK.

 

Functional characterization of the Oesophagostomum dentatum nicotine-sensitive AChR. Claude L. Charvet 1,2*, Elise Courtot 1,2, Hadile Gabaj 1,2, Nicolas Peineau 3, Richard J. Martin 4, Alan P. Robertson 4, Thomas Boulin 5 , Robin N. Beech 6 and Cédric Neveu.  World Association for the Advancement of Veterinary Parasitology Conference, August 2015, Liverpool UK.

 

RNAi competency in adult Ascaris suum - potent, persistent and reproducible knockdown. Ciaran J. McCoy1, Neil D. Warnock2, Louise E. Atkinson2, Erwan Atcheson2, Richard J. Martin3, Alan P. Robertson3, Nikki J. Marks2, Aaron G. Maule2, Angela Mousley2. American Society for Tropical Medicine & Hygiene Annual Meeting, October 2015, Philadelphia PA.

 

In vitro antifilarial activities and metabolomic profiling of extracts of Daniellia oliveri and Psorospermum febrifugum. Melanie Abongwa1, Fidelis Cho-Ngwa2, Godfred A. Ayimele2, Moses Samje2, Smith B. Babiaka2, Ann Perera1, Adrian J. Wolstenholme3, Richard J. Martin1, Alan P. Robertson1. American Society for Tropical Medicine & Hygiene Annual Meeting, October 2015, Philadelphia PA.