Jianqiang Zhang

Education & Certifications  

MD (Preventive Medicine), 1996, Beijing Medical University (now Peking University Health Science Center), School of Public Health, Beijing, China         
MS (Molecular Virology), 1999, Chinese Academy of Preventive Medicine (now China CDC), Institute of Virology, Beijing, China                                         
PhD (Veterinary Science with an emphasis on molecular virology), 2005, University of Kentucky


VDPAM 488 - Laboratory in Clinical Microbiology

Research Focus & Interests  

Diagnostics, molecular epidemiology, pathogenesis and vaccine development of viruses of veterinary significance.

Honors & Awards  

ISU College of Veterinary Medicine Early-Career Achievement in Research Award (2015)
Iowa State University Foreign Travel Award (2015)
Postdoctoral Fellow Travel Award from American Society for Virology (2006; 2009)
Geoffrey C. Hughes Foundation Fellowship, University of Kentucky (1999-2005)


Conference of Research Workers in Animal Diseases (CRWAD)
American Association of Swine Veterinarians (AASV)
American Association of Veterinary Laboratory Diagnosticians (AAVLD)
American Society for Microbiology
American Society for Virology

Selected Publications  

*Zhang J as the corresponding author:

* Zhang J, Gauger P, and Harmon K. (2016). Swine influenza A virus. In Molecular Detection of Animal Viral Pathogens. Edited by Don Liu. (in press)

Lu Z, Sarkar S, Zhang J, and Balasuriya UBR.  (2016). Conserved arginine residues in the carboxyl terminal of equine arteritis virus E protein may play a role in heparin binding but may not affect the viral infectivity in equine endothelial cells. Arch Virol. doi: 10.1007/s00705-015-2733-3 [Epub ahead of print]

Goodell C, Zhang J, Strait E, Harmon K, Patnayak D, Otterson T, Gramer M, Christopher-Hennings J, Clement T, Leslie-Steen P, Hesse R, Anderson J, Skarbek K, Vincent A, Kitikoon P, Swenson S, Jenkings-Moore M, McGill J, Rauh R, Nelson W, O'Connell C, Shah R, Wang C, Main R, Zimmerman J. (2016). Ring test evaluation of the detection of influenza A virus in swine oral fluids by real-time, reverse transcription polymerase chain reaction and virus isolation. Can J Vet Res. 80(1), 12-20

Madson DM, Arruda PHE, Magstadt DR, Burrough ER, Hoang H, Sun D, Bower LP, Bhandari M, Gauger PC, Stevenson GW, Wilberts BL, Wang C, Zhang J, and Yoon KJ. (2016). Characterization of porcine epidemic diarrhea virus isolate US/Iowa/18984/2013 infection in one-day-old caesarian derived colostrum deprived (CDCD) piglets. Vet Pathol. 53(1), 44-52

Jarvis MC, Lam HC, Zhang Y, Wang L, Hesse RA, Hause BM, Vlasova A, Wang Q, Zhang J, Nelson M, Murtaugh M, and Marthaler D. (2015). Genomic and phylogenetic relationships between American and global strains of porcine epidemic diarrhea virus. Prev Vet Med. Nov 10. pii: S0167-5877(15)30045-3. doi: 10.1016/j.prevetmed.2015.11.001. [Epub ahead of print]

Schneider PT, Zhang J, Ramirez A, Wang C, and Holtkamp DJ.  (2015). Evaluation of disinfection protocols to reduce virus transmission via livestock transport vehicles using model trailers in experimental conditions. J Swine Health Prod. 23(6), 306-316

* Zhang J, Piñeyro P, Chen Q, Zheng Y, Li G, Rademacher C, Derscheid R, Guo B, Yoon K, Madson D, Gauger P, Schwartz K, Harmon K, Linhares D, and Main R. (2015). Full-length genome sequences of Senecavirus A from recent idiopathic vesicular disease outbreaks in United States Swine. Genome Announc. 3(6): e01270-15

* Thomas JT, Chen Q, Gauger PC, Gimenez-Lirola LG, Sinha A, Harmon KM, Madson DM, Burrough ER, Magstadt DR, Salzbrenner H, Welch MW, Yoon KJ, Zimmerman JJ, and Zhang J.  (2015). Effect of porcine epidemic diarrhea virus infectious doses on infection outcomes in naïve neonatal and weaned pigs. PLoS ONE. 10(10): e0139266

Rajao DS, Gauger P, Anderson TK, Lewis NS, Abente EJ, Killian ML, Zhang J, and Vincent AL. (2015). Novel human-like swine H3N2 and H3N1 influenza A viruses detected in pigs are virulent and antigenically distinct. J Virol. 89(22), 11213-11222

* Chen Q, Gauger P, Stafne M, Thomas J, Arruda P, Burrough E, Madson D, Brodie J, Magstadt D, Derscheid R, Welch M, and Zhang J.  (2015). Pathogenicity and pathogenesis of a United States porcine deltacoronavirus cell culture isolate in 5-day-old neonatal piglets. Virology. 482, 51-59

Thomas PR, Karriker LA, Ramirez A, Zhang J, Ellingson JS, Crawford K, Bates J, Hammen KJ, and Holtkamp D. (2015). Evaluation of time and temperature sufficient to inactivate porcine epidemic diarrhea virus in swine feces on metal surfaces. J Swine Health Prod. 23(2), 84-90

* Zhang J and Gauger PC. (2014). Isolation of swine influenza virus in cell cultures and embryonated chicken eggs. In Animal Influenza Virus (Second Edition). Edited by Erica Spackman. Methods Mol Biol. 1161, 265-276

* Zhang J and Harmon KM. (2014). RNA extraction from swine samples and detection of influenza A virus in swine by real-time RT-PCR. In Animal Influenza Virus (Second Edition). Edited by Erica Spackman. Methods Mol Biol. 1161, 277-293

Gerber PF, Xiao C-T, Chen Q, Zhang J, Halbur PG, and Opriessnig T. (2014). The spray-drying process is sufficient to inactivate infectious porcine epidemic diarrhea virus in plasma. Vet Microbiol. 174(1-2), 86-92

Opriessnig T, Xiao C-T, Gerber PF, Zhang J, and Halbur PG. (2014). Porcine epidemic diarrhea virus RNA present in commercial spray-dried porcine plasma is not infectious to naïve pigs. PLoS ONE. 9(8): e104766

* Zhang J, Chen Q, Gauger PC, Harmon KM, and Yoon KJ. (2014). Reply to ‘Classification of emergent U.S. strains of porcine epidemic diarrhea virus by phylogenetic analysis of nucleocapsid and ORF3 genes’. J Clin Microbiol. 52(9), 3511-3514

Balasuriya UB, Zhang J, Go YY, and MacLachlan NJ (2014). Experiences with infectious cDNA clones of equine arteritis virus: lessons learned and insights gained. Virology. 462-463C, 388-403

* Li G, Chen Q, Harmon KM, Yoon KJ, Schwartz KJ, Hoogland M, Gauger PC, Main RG, and Zhang J. (2014). Full-length genome sequence of porcine deltacoronavirus strain USA/IA/2014/8734. Genome Announc. 2(2): e00278-14

Lowe J, Gauger P, Harmon K, Zhang J, Connor J, Yeske P, Loula T, Levis I, Dufresne L, and Main R. (2014). The role of harvest plant lairage and transportation in propagating the initial stages of an outbreak of porcine epidemic diarrhea virus in the United States in 2013. Emerg Infect Dis. 20(5), 872-874

* Chen Q, Li G, Stasko J, Thomas JT, Stensland WR, Pillatzki AE, Gauger PC, Schwartz KJ, Madson D, Yoon KJ, Stevenson GW, Burrough ER, Harmon KM, Main RG, and Zhang J. (2014). Isolation and characterization of porcine epidemic diarrhea viruses associated with the 2013 disease outbreak among swine in the United States. J Clin Microbiol. 52(1), 234-243

Zhang J, Go YY, Huang C, Meade BJ, Lu Z, Snijder EJ, Timoney PJ, and Balasuriya UBR. (2012). Development and characterization of an infectious cDNA clone of the modified live virus vaccine strain of equine arteritis virus. Clin Vaccine Immunol. 19 (8), 1312-1321

Lu Zǂ, Zhang Jǂ, Huang C, Go YY, Faaberg KS, Rowland RR, Timoney PJ, and Balasuriya UBR. (2012). Chimeric viruses containing the N-terminal ectodomains of GP5 and M proteins of porcine reproductive and respiratory syndrome virus do not change the cellular tropism of equine arteritis virus. Virology. 432 (1), 99-109 [ǂ The first two authors should be acknowledged as joint first authors]

Zhang J, Timoney PJ, Shuck KM, Seoul G, Go YY, Lu Z, Powell DG, Meade BJ, and Balasuriya UBR. (2010). Molecular epidemiology and genetic characterization of equine arteritis virus isolates associated with the 2006/2007 multi-state disease occurrence in the USA. J Gen Virol. 91 (Pt9), 2286-2301

Go YY, Zhang J, Timoney PJ, Cook RF, Horohov DW, and Balasuriya UBR. (2010). Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes. J Virol. 84 (10), 4898-4911

Zhang J, Stein DA, Timoney PJ, and Balasuriya UBR. (2010). Curing of HeLa cells persistently infected with equine arteritis virus by a peptide-conjugated morpholino oligomer. Virus Res. 150 (1-2), 138-142

Zhang J, Timoney PJ, MacLachlan NJ, and Balasuriya UBR. (2008). Identification of an additional neutralization determinant of equine arteritis virus. Virus Res. 138 (1-2), 150-153

Zhang J, Timoney PJ, MacLachlan NJ, McCollum WH, and Balasuriya UBR. (2008). Persistent equine arteritis virus infection in HeLa cells. J Virol. 82 (17), 8456-8464

Zhang J, Go YY, MacLachlan NJ, Meade BJ, Timoney PJ, and Balasuriya UBR. (2008). Amino acid substitutions in the structural or non-structural proteins of a vaccine strain of equine arteritis virus are associated with its attenuation. Virology. 378 (2), 355-362

Zhang J, Guy JS, Snijder EJ, Denniston DA, Timoney PJ, and Balasuriya UBR. (2007). Genomic characterization of equine coronavirus. Virology. 369 (1), 92-104

Zhang J, Miszczak F, Pronost S, Fortier C, Balasuriya UBR., Zientara S, Fortier G, and Timoney PJ (2007). Genetic variation and phylogenetic analysis of 22 French isolates of equine arteritis virus. Arch Virol. 152 (11), 1977-1994